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Item Metadata only Study of the dynamics and localization of MinD protein through single particle tracking(Mahidol University. Mahidol University Library and Knowledge Center, 2008) Somrit Unai; Narin NattavutItem Metadata only Identification of critical amino acids of p12DOC-1 for interaction with DOC-1R(Mahidol University. Mahidol University Library and Knowledge Center, 2004) Kanokporn Kampoo; Waranun BuajeebPublication Metadata only Changes in integument histology and protein expression related to the molting cycle of the black tiger shrimp, Penaeus monodon(2007-09-01) Waraporn Promwikorn; Pornpimol Kirirat; Pranom Intasaro; Boonsirm Withyachumnarnkul; Prince of Songkla University; Mahidol UniversityWe investigated changes in the histology and protein expression in the epidermis and sub-epidermis of the black tiger shrimp (Penaeus monondon) during the molting cycle. The epidermis consists of a cell layer located beneath the cuticle, while... the sub-epidermis is mainly composed of sub-epidermal cells and tegumental glands. During the molting cycle, the epidermal cells increase in cell height and number, and the sub-epidermis increases in its storage of carbohydrate, protein, mucus, and otherItem Metadata only Caffeine causes cell cycle arrest at G0/G1 and increases of ubiquitinated proteins, ATP and mitochondrial membrane potential in renal cells(2023-01-01) Kanlaya R.; Subkod C.; Nanthawuttiphan S.; Thongboonkerd V.; Mahidol Universityat the protein level. Cellular proteome of MDCK cells treated with caffeine at a physiologic concentration (100 μM) for 24 h was analyzed comparing with that of untreated cells by label-free quantitative proteomics. From a total of 936 proteins identified..., comparative analysis revealed significant changes in levels of 148 proteins induced by caffeine. These significantly altered proteins were involved mainly in proteasome, ribosome, tricarboxylic acid (TCA) (or Krebs) cycle, DNA replication, spliceosomeItem Metadata only Ethanolic extract of Halymenia durvillei induced G2/M arrest and altered the levels of cell cycle regulatory proteins of MDA-MB-231 triple-negative breast cancer cells(2023-05-01) Settacomkul R.; Sangpairoj K.; Phuagkhaopong S.; Meemon K.; Niamnont N.; Sobhon P.; Vivithanaporn P.; Mahidol University. The present study investigated the effect of HDET on the viability and proliferation of MDA-MB-231, a triple-negative breast cancer (TNBC) cell line. Experimental approach: Cell proliferation and cell cycle analysis were determined by flow cytometry and cell... cycle regulatory protein expression levels were then determined by Western blotting. The presence of reactive oxygen species (ROS) was evaluated by dichlorofluorescein, followed by analyzing changes in gene expression of antioxidant enzymes using a realPublication Metadata only Prolonged K+deficiency increases intracellular ATP, cell cycle arrest and cell death in renal tubular cells(2017-09-01) Kedsarin Fong-ngern; Nardtaya Ausakunpipat; Nilubon Singhto; Kanyarat Sueksakit; Visith Thongboonkerd; Mahidol UniversityOntology (GO) classification and protein network analysis revealed that majority of the altered proteins participated in metabolic process, whereas the rest involved in cellular component organization/biogenesis, cellular process (e.g., cell cycle..., regulation of cell death), response to stress, and signal transduction. Interestingly, ATP measurement revealed that intracellular ATP production was increased in CLK and maximum in CKD. Flow cytometry showed cell cycle arrest at S-phase and G2/M-phase in CLKPublication Metadata only Cadmium induces apoptotic program imbalance and cell cycle inhibitor expression in cultured human astrocytes(2019-01-01) Dusadee Ospondpant; Suttinee Phuagkhaopong; Kran Suknuntha; Kant Sangpairoj; Thitima Kasemsuk; Chutima Srimaroeng; Pornpun Vivithanaporn; University of Wisconsin School of Medicine and Public Health; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Faculty of Medicine, Thammasat University; Mahidol University; Burapha University; Chiang Mai Universityon proteins regulating oxidative stress, apoptosis, and cell proliferation in astrocytes are less known. The present study hypothesized that cadmium alters levels of antioxidant enzymes, apoptotic regulator proteins, and cell cycle inhibitor proteins...), downregulation of B-cell lymphoma 2 (Bcl-2), and upregulation of Bcl-2-associated X protein (Bax). Moreover, cadmium suppressed astrocytic proliferation by inducing S and G2/M phase cell cycle arrest and promoting p53, p21, and p27 expression. In conclusionItem Metadata only Proteomic profiling reveals neuronal ion channel dysregulation and cellular responses to DNA damage-induced cell cycle arrest and senescence in human neuroblastoma SH-SY5Y cells exposed to cypermethrin(2022-12-01) Promthep K.; Nopparat C.; Mukda S.; Pannengpetch S.; Wisomka P.; Chantadul V.; Phanchana M.; Panmanee J.; Mahidol Universitydownregulated in CYP-treated cells. After 48 h of CYP exposure, cell viability was reduced with flattened and enlarged morphology. The levels of 23 proteins regulating cell cycle processes were altered in CYP-treated cells, according to a proteomic study... neuronal development, increasing cellular oxidative stress, and apoptosis. However, little is known about how it affects the expression of channel proteins involved in synaptic transmission, as well as the effects of cypermethrin on DNA damage and cellItem Metadata only Cyclin D1 regulates S3291 phosphorylation of BRCA2(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Chonvara Chalermrujinanant; Siwanon Jirawatnotai; Uraiwan Panich; Donniphat DejsuphongCyclin D1 is a cell cycle regulatory protein. It is also known as a cancer causing protein in human. A mechanism by which cyclin D1 promotes cancer formation is still unclear. Recently, cyclin D1 was established for a novel function in DNA repair... by which cyclin D1 cooperates with the HR DNA repair machineries, emphasizing on an interaction between cyclin D1 and the breast cancer type 2 susceptibility protein (BRCA2). Additionally, the biological significance of their interaction in cell cycleItem Metadata only Novel Midkine Inhibitor Induces Cell Cycle Arrest and Apoptosis in Multiple Myeloma(2024-03-01) Cheevapruk K.; Ueno M.; Sungwan P.; Sittithumcharee G.; Kariya R.; Sampattavanich S.; Okada S.; Cheevapruk K.; Mahidol University. Cell cycle and apoptosis were evaluated using flow cytometry. To further investigate the inhibitory mechanism, western blotting was used to detect cell cycle-related proteins, pro-apoptotic proteins, and anti-apoptotic proteins. Results: iMDK inhibits... MM cell proliferation in a dose- and time-dependent manner, inducing cell cycle arrest and apoptosis. The reduction in Cdc20 expression by iMDK treatment leads to G2/M phase cell cycle arrest. Furthermore, iMDK down-regulates anti-apoptotic proteins