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Publication Metadata only Is Sabin-Feldman dye test using T. gondii tachyzoites from animal inoculation still the best method for detecting Toxoplasma gondii antibodies?(2010) Ruenruetai Udonsom; Ruangrat Buddhirongawatr; Yaowalark Sukthana; Mahidol University. Mahidol University International College.Although the Sabin-Feldman dye test is the gold standard for detecting Toxoplasma antibodies in human, it is performed only in reference laboratories because live virulent T. gondii are used for the test. We collected 210 human serum samples... and tested them by the dye test using in vivo tachyzoites (conventional method) than compared these results with three other methods: a dye test using cell culture-derived T. gondii tachyzoites and indirect immunofluorescent antibody tests (IFAT) usingPublication Metadata only Is sabin-feldman dye test using T. Gondii tachyzoites from animal inoculation still the best method for detecting toxoplasma gondii antibodies?(2010-09-01) Ruenruetai Udonsom; Ruangrat Buddhirongawatr; Yaowalark Sukthana; Mahidol UniversityAlthough the Sabin-Feldman dye test is the gold standard for detecting Toxoplasma antibodies in human, it is performed only in reference laboratories because live virulent T. gondii are used for the test. We collected 210 human serum samples... and tested them by the dye test using in vivo tachyzoites (conventional method) then compared these results with three other methods: a dye test using cell culture-derived T. gondii tachyzoites and indirect immunofluorescent antibody tests (IFAT) usingPublication Metadata only A comparison of techniques for assessment of coronal dye leakage(2002-01-01) Suwit Wimonchit; Siriporn Timpawat; Noppakun Vongsavan; Khon Kaen University; Mahidol UniversityThis study was designed to compare different techniques for coronal dye leakage testing. One hundred and fifty extracted human anterior teeth were fully instrumented and randomly divided into two experimental groups of 60 teeth each, plus two groups... condensation and AH Plus as root canal sealer. Each experimental group was divided into three subgroups of 20 teeth each for three methods of testing coronal dye leakage: passive dye penetration, penetration with vacuum applied, and fluid filtration methodsPublication Metadata only Comparison of indirect immunofluorescent antibody test and Sabin-Feldman dye test for detection of Toxoplasma gondii antibody in Thai pregnant women(2004-12-01) Uraiwan Krainara; Supatra Thongrungkiat; Wipawee Usawattanakul; Porntip Petmitr; Yaowalak Sukthana; Department of Protozoology; Mahidol UniversityThe aim of this study is to determine the specific IgG antibody for Toxoplasma gondii in pregnant Thai women by IFAT, using formalin-fixed whole tachyzoites and comparing the sensitivity and specificity with those of the Sabin-Feldman dye test (SFDT... and 95.9% specificity, when compared with Sabin-Feldman dye test. IFAT is not time-consuming, easy to perform, and formalin-fixed whole antigens can be kept for a long time. IFAT, therefore, is recommended as a selective test, which can be usedPublication Metadata only Decolorization of basic, direct and reactive dyes by pre-treated narrow-leaved cattail (Typha angustifolia Linn.)(2004-09-01) Duangrat Inthorn; Siritham Singhtho; Paitip Thiravetyan; Eakalak Khan; Mahidol University; King Mongkuts University of Technology Thonburi; North Dakota State University(3, 5, 7, and 9) was tested. Desorption of the adsorbed dyes was also investigated. The type of NLC treatment and pH of the dye solution had little effect on removal of basic dyes, and efficiencies ranged from 97% to 99% over the range of pH used... dye (Basilen Red M-5B). There was mutual attraction between negatively charged direct dye molecules and positively charged molecules on the surface of the FH-treated cattail. In tests of desorption of dyes from cattail in distilled waterItem Metadata only In vitro culture of toxoplasma gondii for dye test in captive wild felids(2005) Ruangrat Buddhirongawatr; รวงรัตน์ พุทธิรงควัตร; Siriporn Tangsudjai; Kridsada Chaichoune; Rusmeepen Poonakngeng; Rattapan Pattanarangsan; Roschong Boonyarittichaikij; Charoonluck Sangloung; Nitipan Tantawiwattananon; นิธิพันธ์ ตัณฑะวิวัฒนานนท์; Yaowalark Sukthana; เยาวลักษณ์ สุขธนะ; Mahidol University. Faculty of Tropical Medicine. Department of Medical EntomologyLa culture was used in the Sabin-Feldman dye test for detection of T. gondii antibody Serum samples of 21 captive wild felids including on fishing cat (Prion nailurus uiverrina), one leopard (Panthera pardus), two flat-headed cat (Prion nailurus uiverrina... be used successfully as a source of live organisms in a gold standard Sabin-Feldman dye test which provides more simple, cheaper and less ethically sensitive than in vivo inoculation.Item Metadata only Comparison of indirect fluorescence antibody and the sabin-feldman dye test for detection of Toxoplasma gondii antibody in pregnant Thai women(Mahidol University. Mahidol University Library and Knowledge Center, 2004) Uraiwan Krainara; Yaowalark SukthanaPublication Metadata only Comparison between Sulphorhodamine-B dye staining and51Cr-release method in cytotoxicity assay of endodontic sealers(1997-01-01) La Ongthong Vajrabhaya; Mahidol UniversityThe aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH26. Monolayers (5 x... 105cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37°C in 5% CO2for 24 h in the presence of each sealer, cells were stained with 0.4% SRB and the absorbance at 540 nm determined as measure of cell viabilityItem Metadata only Effect of azo dye treatment on the detection of human (OC43) coronavirus surrogate and SARS-CoV-2 viability based on an in-house photoactivator device(2024-08-01) Niyomdecha N.; Noisumdaeng P.; Boonarkart C.; Auewarakul P.; Niyomdecha N.; Mahidol Universityin-house photoactivator device. The factors affecting azo dye efficacy were optimized in tests with free viral RNA and virus suspension. A propidium monoazide (PMAxx) had a better performance than ethidium monoazide bromide (EMA) in the exclusion of false... for a complete suppression of inactivated virus suspension at 102 pfu/100 µl. Additionally, various techniques of dye combinations, surfactant cotreatment, and double light exposure were tested to improve the result. Only the combination of 1 mM PMAxxPublication Metadata only Predictive value of latex agglutination test in serological screening for Toxoplasma gondii(2001-06-01) Yaowalark Sukthana; Thaiyooth Chintana; Waraporn Supatanapong; Chutatip Siripan; Amorn Lekkla; Rachatawan Cheabchalrad; Mahidol University-Feldman dye test. Five hundred serum samples were collected from 200 blood donors and 100 each from pregnant women, kidney recipients and HIV infected persons. Eighty (16.0 %) out of 500 subjects were positive for Toxoplasma IgG antibody by ToxoScreen DA... (bioMerieux) compared with 57(11.4%) by Sabin-Feldman dye test. The sensivity and specificity of Toxo-Screen DA (bioMerieux) were 100 % and 94.8 % respectively which were similar to previous reports from the area of high prevalence of Toxoplasma