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Item Metadata only Studies of biological significance of apoptosis upon white-spot syndrome virus infection in penaeid shrimp(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Anchukorn Rijiravanich; Boonsirm WithyachumnarnkulItem Metadata only Development of double-stranded RNA-delivery system for control of Laem-Singh virus (LSNV) in Thai penaeus monodon(Mahidol University. Mahidol University Library and Knowledge Center, 2013) Thitiporn Thammasorn; Suparerk Borwornpinyo; Vanvimon Saksmerprome; Boonsirm WithyachumnarnkulRNA interference (RNAi) technology through the trigger of double-stranded RNA (dsRNA) has been applied to inhibit viral replication in penaeid shrimp. In this study, we have developed a new methodology to deliver dsRNA for controlling Laem-Singh... detectable in the gut of Artemia under confocal microscope were evident for the successful encapsulation. Second, the Artemia enrichment process was performed using E. coli producing LSNV-specific dsRNA. By reverse transcription-PCR (RT-PCR), dsRNA-LSNVItem Open Access Production of co-expressed double-stranded RNA and virus-like particle in a novel escherichia coli strain for protection against white spot syndrome virus in shrimp(Mahidol University, 2021) Kitti Wuthisathid; Ornchuma Itsathitphaisarn; Thawatchai Chaijarasphong; Kallaya Sritunyalucksana; Charoonroj Chotwiwatthanakun; Monsicha Somrit, dsRNA-based platforms are among the promising tools for triggering an antiviral response in shrimp through an RNA interference (RNAi) pathway. The main obstacle to utilizing the RNAi strategy in the shrimp aquaculture industry is the lack of a low...-cost, efficient, and practical delivery approach. Recently, virus-like particles (VLPs) have been used as nanocarriers to deliver dsRNA into shrimp tissues. The VLP-based delivery system extends the half-life of the targeted dsRNA, provides broad tissuePublication Metadata only Inhibition of Plasmodium falciparum proliferation in vitro by double-stranded RNA nanoparticle against malaria topoisomerase II(2016-05-01) Pongsopee Attasart; Siriwan Boonma; Panya Sunintaboon; Dolpawan Tanwilai; Jinrapa Pothikasikorn; Wilai Tienrungroj Noonpakdee; Mahidol University-stranded interfering RNA to target cells. We have employed a long double-stranded (dsRNA) targeting the coding region of PfTOP2 that is complexed with chitosan nanoparticles in order to interfere with the cognate mRNA expression and examined its effect on P... is in the development of antimalarials based on RNA interference targeting expression of malaria parasite vital genes, viz. DNA topoisomerase II gene (PfTOP2). Biodegradable chitosan nanoparticle system has proven to be effective in delivering DNA and small doublePublication Metadata only Inhibition of Taura syndrome virus replication in Litopenaeus vannamei through silencing the LvRab7 gene using double-stranded RNA(2011-07-01) Chalermporn Ongvarrasopone; Pipop Saejia; Mayuree Chanasakulniyom; Sakol Panyim; Mahidol UniversityTaura syndrome virus (TSV) is a major cause of high mortality in Pacific white shrimp (Litopenaeusvannamei, Lv). Previously, silencing of Penaeus monodon Rab7 (PmRab7) by injecting double-stranded RNA corresponding to PmRab7 (dsRNA-PmRab7) prevented... white spot syndrome virus or yellow head virus infection. Rab7 is proposed to be involved in intracellular trafficking of the viruses. This study aimed to investigate whether knockdown of Rab7 in L. vannamei by dsRNA-PmRab7 could inhibit replicationPublication Metadata only Inhibition of Penaeus monodon densovirus replication in shrimp by double-stranded RNA(2010-03-24) Pongsopee Attasart; Rossukon Kaewkhaw; Chaweewan Chimwai; Ukrit Kongphom; Orathai Namramoon; Sakol Panyim; Mahidol UniversityStunted shrimp caused by Penaeus monodon densovirus (PmDNV) infection is one of the main problems leading to a significant economic loss in Thailand. To control this pandemic disease, a double-stranded-RNA-mediated virus-specific gene silencing.... After allowing viral replication for two weeks, the suppression effect by each dsRNA was evaluated by semi-quantitative PCR and compared with the control. A reduction of PmDNV in shrimp treated with each dsRNA was observed. In contrast, a high levelItem Metadata only Transgenic Microalgae Expressing Double-Stranded RNA as Potential Feed Supplements for Controlling White Spot Syndrome in Shrimp Aquaculture(2023-08-01) Charoonnart P.; Taunt H.N.; Yang L.; Webb C.; Robinson C.; Saksmerprome V.; Purton S.; Mahidol UniversityViral infection of farmed fish and shellfish represents a major issue within the aquaculture industry. One potential control strategy involves RNA interference of viral gene expression through the oral delivery of specific double-stranded RNA (dsRNA... use to protect shrimp against white spot syndrome virus (WSSV). A new strategy for dsRNA synthesis was developed that uses two convergent copies of the endogenous rrnS promoter to drive high-level transcription of both strands of the WSSV gene elementPublication Metadata only Induction of Ovarian Maturation and Spawning in Penaeus monodon Broodstock by Double-Stranded RNA(2011-04-01) Supattra Treerattrakool; Sakol Panyim; Apinunt Udomkit; Mahidol Universitypractice in Penaeus monodon hatchery to induce ovarian maturation and spawning. Our previous report showed that silencing of GIH expression by GIH-specific double-stranded RNA (GIH-dsRNA) resulted in an increased expression level of vitellogenin in P.... monodon, thus suggesting that GIH-dsRNA could be an alternative method to induce ovarian maturation in female P. monodon broodstock. In this study, we further demonstrated that a single injection of GIH-dsRNA into previtellogenic female P. monodonPublication Metadata only Inhibition of Plasmodium falciparum proliferation in vitro by double-stranded RNA directed against malaria histone deacetylase(2009-04-03) N. Sriwilaijaroen; S. Boonma; P. Attasart; J. Pothikasikorn; S. Panyim; W. Noonpakdee; Faculty of Medicine, Thammasat University; Mahidol Universityhistone acetylase and deacetylase (HDAC). We have used long PfHDAC-1 double-stranded (ds)RNA to interfere with its cognate mRNA expression and determined the effect on malaria parasite growth and development. Chloroquine- and pyrimethamine-resistant... Plasmodium falciparum K1 strain was exposed to 1-25 μg of dsRNA/ml of culture for 48 h and growth was determined by [3H]-hypoxanthine incorporation and microscopic examination. Parasite culture treated with 10 μg/ml pfHDAC-1 dsRNA exhibited 47% growthPublication Metadata only Improved preventive and curative effects of YHV infection in Penaeus monodon by a combination of two double stranded RNAs(2011-04-04) Pratsaneeyaporn Posiri; Chalermporn Ongvarrasopone; Sakol Panyim; Mahidol UniversityYHV causes a severe mortality in Penaeus monodon resulting in a high economic loss of shrimp culture industry. Presently, there is no effective means to prevent or cure YHV infection in shrimp. Double stranded RNA (dsRNA) targeting viral genes... exhibited a good protection of the YHV infection. DsRNA targeting a shrimp gene PmRab7 similarly gave a good preventive effect. In this study, a combination of dsRNA targeting viral and host genes showed improved prevention and cure of the YHV infection