Browsing by Author "บุษบา ฤกษ์อำนวยโชค"

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    PublicationOpen Access
    Detection of CEBPA Mutation Gene in Acute Myeloid Leukemia Patients
    (2017) Takol Chareonsirisuthigul; Sutada Magmuang; Suporn Chuncharunee; Budsaba Rerkamnuaychoke; ถกล เจริญศิริสุทธิกุล; สุธาดา มากเมือง; สุภร จันท์จารุณี; บุษบา ฤกษ์อำนวยโชค; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Pathology; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Medicine
    Background: The mutations of CCAAT/enhancer binding protein-alpha (CEBPA) gene are evaluated as favorable prognostic tools for acute myeloid leukemia (AML) patients. The gold standard method for detection of CEBPA gene mutations is direct sequencing. This method has some disadvantages, and CEBPA mutations can occur across the whole gene, and there should be a screening test before designating the type of mutation by direct sequencing. Objective: This study was to evaluate the ability of denaturing high-performance liquid chromatography (DHPLC) for screening CEBPA mutations. Method: The coding region of CEBPA gene in 114 AML patients and 40 normal controls were screened by DHPLC and confirmed by direct sequencing. Results: Our results demonstrated that DHPLC is a useful screening test to detect CEBPA gene mutations in AML patients. Fifteen types of CEBPA gene mutations including insertion, duplication, deletion, and substitution were also detected by DHPLC. Conclusion: A combination of DHPLC and direct sequencing is an appropriate approach for detecting CEBPA mutations.
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    PublicationOpen Access
    MicroRNA Expression of Primary and Metastatic Colorectal and Breast Carcinoma
    (2022) Thaniya Sricharunrat; Artit Jinawath; Pattana Sornmayura; Sansanee Wongwaisayawan; Budsaba Rerkamnuaychoke; ธนิยะ ศรีจรุณรัตน์; อาทิตย์ จินาวัฒน์; พัฒนา ศรมยุรา; ศันสนีย์ วงศ์ไวศยวรรณ; บุษบา ฤกษ์อำนวยโชค; Chulabhorn Hospital, Chulabhorn Royal Academy. Pathology and Forensic Science Department; Mahidol University. Faculty of Medicine Ramathibodi Hospital.Department of Pathology; Rangsit University. Department of Medical Science, Faculty of Science
    Background: Colorectal and breast carcinoma are frequently diagnosed cancers. At advanced stages, cancers metastasize to certain organs resulting in loss of function of these organs, and eventually death. Therefore, there is a specific need for the prognosis of these cancers. Currently, microRNAs (miRNAs), have emerged as a new target of cancer-specific biomarker. Objective: To examine the expression of miRNA in primary and metastatic breast and colorectal cancers. Methods: This study investigated the expression of 6 miRNAs (miR-10b, miR-21, miR-145, miR-155, miR-200c, and miR-373) in formalin fixed paraffin embed tissues from pairs of normal tissues with primary and metastatic tumor samples of breast and colorectal carcinoma cases in Ramathibodi Hospital, Thailand by real-time RT-PCR. Results: Among 6 miRNAs, miR-145 decreased significantly in all samples of primary and metastatic colorectal and breast carcinoma. There was significantly decreased expression of miR-145 in metastatic colorectal carcinoma compared to their primary colorectal carcinoma (P < .05). Whereas miR-10b, miR-155, and miR-200c showed a decreased expression; miR-21, and miR-373 showed an increased expression in the majority of cases. Unlike miR-145, other miRNAs showed no significant difference of expression (P > .05). Conclusions: This finding indicates that miR-145 may be the potential metastatic biomarker. Decrease of miR-145 could be applied to the prognosis and target for therapy of breast and colorectal carcinoma.
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    PublicationOpen Access
    The Use of Quantitative Fluorescence PCR for Common Aneuploidy Detection in Prenatal Diagnosis
    (2557) Nittaya Rodratn; Jittima Shotivaranon; Takol Chareonsirisuthigul; Panyu Panburana; Budsaba Rerkamnuaychoke; นิตยา รอดรัตน์; จิตติมา โชติวรานนท์; ถกล เจริญศิริสุทธิกุล; พัญญู พันธ์บูรณะ; บุษบา ฤกษ์อำนวยโชค; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Pathology; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Obstetrics and Gynecology
    Background: The major type of chromosomal abnormality is aneuploidy which has the chance both too many and too few numbers of chromosomes. Trisomy is a common aneuploidy in prenatal diagnostic problem. The gold standard for detection of this abnormality is karyotype analysis. However, this method has many processes that cause time-consuming and wanted the experience of laboratory personnel. All of these issues lead to the increasing of parental anxiety. Objective: The purpose of this study was to evaluate a molecular method, QF-PCR, to detect common aneuploidy in prenatal diagnosis. Methods: We performed this assay in 100 prenatal samples. Then, the prenatal DNA samples were extracted and detected for aneuploidy status by QF-PCR. These results were compared with karyotyping results. Results: 99 of 100 total samples can be interpreted. There were 92 cases with normal and aneuploidy karyotypes had the same result with QF-PCR. Mosaicism could be detected too. In 4 cases of unbalance autosomal rearrangements the QF-PCR results were normal. In 3 cases of marker chromosome, 2 cases were interpreted as normal and other the QF-PCR result was reported to be monosomy X. In the case of pentasomy X, OF-PCR result was reported to be triple X. In one case was inconclusive from maternal cell contamination. Conclusions: QF-PCR is an alternative method to detect common aneuploidy in prenatal samples. Because there was no false positive result in normal karyotype samples and cases of common aneuploidy showed no false negative result as well.
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    PublicationOpen Access
    Validation of Molecular Karyotyping Techniques for Rapid Prenatal Diagnosis of Common Aneuploidies
    (2016) Prapatsorn Areesirisuk; Takol Chareonsirisuthigul; Somsri Pitukkijronnakorn; Punyu Panburana; Budsaba Rerkamnuaychoke; ประภัสสร อารีสิริสุข; ถกล เจริญศิริสุทธิกุล; สมศรี พิทักษ์กิจรณกร; พัญญู พันธ์บูรณะ; บุษบา ฤกษ์อำนวยโชค; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Pathology; Bumrungrad International Hospita; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Obstetrics and Gynecology
    BACs-on-Beads (BoBs) technology and Quantitative fluorescent PCR (QF-PCR) are recent molecular karyotping methods which have been used for prenatal diagnosis of the most common aneuploidies. Both of them are rapid, cost-effective and suitable for automation and can detect most abnormalities diagnosed by conventional karyotyping. The objective of this study was to evaluate the performance of bote molecular-based techniques for the detection of chromosomes 13, 18, 21, X and Y. The results obtained from 22 prenatal samples in which BACs-on-Beads technology (KaryoLiteTM BoBs and PrenatalTM BoBs), QF-PCR and conventional karyotype had been performed. We found that concordant KaryoLiteTM BoBs, PrenatalTM BoBs, QF-PCR and karyotype results were obtained in 95.5% (21/22) of the commonaneuploidies. Only a 49,xxxxx sample could not be detected by BoBs assay and QF-PCR. In conclusions, BoBs technology and QF-PCR are the reliable methods to detect common aneuploidies and should replace conventional sytogenetic analysis whenever prenatal testing is performed solely because of an increased risk of chromosomes 13, 18, 21, X and Y. Cytogenetic follow-up of molecular karyotyping findings is recommended to rule out mosaicism, ,aternal cell contamination, balanced rearrangement and polyploidy.
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    ItemOpen Access
    วิทยาการก้าวหน้าด้านนิติพันธุศาสตร์
    (2561) บุษบา ฤกษ์อำนวยโชค; มหาวิทยาลัยมหิดล. คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี