Browsing by Author "Charnnarong Sanghirun"

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    Acetylcholinesterase and cholinesterase activities in giardia lamblia trophozoites cultured in vitro
    (2004) Cheeraratana Cheeramakara; จีระรัตน์ จีระมะกร; Apichart Nontprasert; อภิชาต นนท์ประเสริฐ; Kriyaporn Songmuaeng; กริยาพร สองเมือง; Wanyarat Thanomsak; วัลยารัตน์ ถนอมศักดิ์; Chutatip Siripanth; จุฑาทิพ ศิริพันธุ์; Nopachai Suthisai; นภชัย สุทธิสัย; Charnnarong Sanghirun; ชาญณรงค์ แสงหิรัญ; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Radioisotope; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology
    Axenic culture of Giardia lamblia trophozites in vitro was conducted from patients infected with Giardia lamblia. The cholinesterase (ChE) and acetylcholinesterase (AChE) activities were determined in cultured Giardia trophozoites. The enzyme activities were found in boh tissue-bound and tissue-free forms. There was low ChE and AChE enzyme activities in trophozoites (1.6095 ± 1.0981; 0.9626 ± 0.1322; 0.1387 ± 0.0783; 0.0752 ± 0.0877 Units/ mg protein, respectively). The tissue-bound and tissue-free ChE enzymes were found in a greater amount than tissue-bound and tissue-free AChE enzymes. The presence of these enzymesin Giardia may involved in the motility of Giardia trophozoites.
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    Elevation of serum transcobalamin II in patients with scrub typhus
    (2005-01-01) Cheeraratana Cheeramakara; Wanyarat Thanomsak; Kriyaporn Songmeang; Apichart Nontprasert; Charnnarong Sanghirun; Nopachai Suthisai; Suvit Areekul; Mahidol University
    Serum transcobalamin II levels were measured in scrub typhus patients. Eighteen out of fifty-two patients admitted to Maharat Nakhon Ratchasima Hospital were diagnosed with scrub typhus infection. The serum unsaturated vitamin B12 binding protein (UBBC) and total vitamin B12 binding protein (TBBC) levels in these patients were significantly higher than in normal subjects (p<0.001). The mean serum transcobalamin II level in the typhus patients was also significantly higher than in the normal subjects (p=0.004). There was a significant correlation between serum TCII levels and typhus IgM or IgG titers (p<0.05), but not to total IgM levels. These findings indicate that patients with scrub typhus had stimulation of the recticuloendothelial system as a result of a considerable increase in transcobalamin II levels.
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    Study on serum transcobalamin II in patients with murine typhus
    (2005) Cheeraratana Cheeramakara; จีระรัตน์ จีระมะกร; Kriyaporn Songmeang; กริยาพร สองเมือง; Wanyarat Thanomsak; วัลยารัตน์ ถนอมศักดิ์; Apichart Nontpraset; อภิชาต นนท์ประเสริฐ; Nopachai Suthisai; นภชัย สุทธิสัย; Charnnarong Sanghirun; ชาญณรงค์ แสงหิรัญ; Suvit Areekul; สุวิทย์ อารีย์กูล; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Radioisotopes; Mahidol University. Faculty of Tropical Medicine. Department of Clinical Tropical Medicine
    We measured the serum transcobalamin II in murine typhus- infected patients (n = 16) admitted to the Hospital for Tropical Diseases in 1996-1997, compared with healthy controls (n = 60). The results showed that the transcobalamin II (TCII) and total serum unsaturated vitaminB12 binding capacity (UBBC) in patients with murine typhus (2,126.5 pg/ml, range 1,262-4,568 and 3,771.5 pg/ml, range 1,576-6,763 pg/ml) were statistically significantly higher than normal subjects (987.5 pg/ml, range 678-2,000 pg/ml and 1,402 pg/ml, range 932-2,470 ml) (p<0.001). Serum TCII levels in patients (63%) were elevated during the febrile period and returned to normal post-treatment. These findings suggest that patients with murine typhus had stimulation of reticulo-endothelial system, spleen, mesenteric lymph nodes, liver and skin and then released TCII into the blood circulation. The elevation in TCII may be used for confirming a diagnosis of murine typhus.