Browsing by Author "D. V. Renno"

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    Transformation of Aspergillus oryzae with a dominant selectable marker
    (1991-01-01) S. Cheevadhanarak; G. Saunders; D. V. Renno; T. W. Flegel; G. Holt; University of Westminster; Mahidol University
    Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991.