Publication: Transformation of Aspergillus oryzae with a dominant selectable marker
Issued Date
1991-01-01
Resource Type
ISSN
01681656
Other identifier(s)
2-s2.0-0025736709
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Biotechnology. Vol.19, No.1 (1991), 117-122
Suggested Citation
S. Cheevadhanarak, G. Saunders, D. V. Renno, T. W. Flegel, G. Holt Transformation of Aspergillus oryzae with a dominant selectable marker. Journal of Biotechnology. Vol.19, No.1 (1991), 117-122. doi:10.1016/0168-1656(91)90079-B Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22005
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Title
Transformation of Aspergillus oryzae with a dominant selectable marker
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Abstract
Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991.
