Publication:
Transformation of Aspergillus oryzae with a dominant selectable marker

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dc.contributor.authorS. Cheevadhanaraken_US
dc.contributor.authorG. Saundersen_US
dc.contributor.authorD. V. Rennoen_US
dc.contributor.authorT. W. Flegelen_US
dc.contributor.authorG. Holten_US
dc.contributor.otherUniversity of Westminsteren_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-08-10T08:30:49Z
dc.date.available2018-08-10T08:30:49Z
dc.date.issued1991-01-01en_US
dc.description.abstractAspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991.en_US
dc.identifier.citationJournal of Biotechnology. Vol.19, No.1 (1991), 117-122en_US
dc.identifier.doi10.1016/0168-1656(91)90079-Ben_US
dc.identifier.issn01681656en_US
dc.identifier.other2-s2.0-0025736709en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/22005
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025736709&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleTransformation of Aspergillus oryzae with a dominant selectable markeren_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025736709&origin=inwarden_US

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