Browsing by Author "Dhanpat K. Kochar"

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    Performance of binaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects
    (2015-01-01) Lyda Osorio; Nick Carter; Preetam Arthur; Germana Bancone; Sowmya Gopalan; Sandeep K. Gupta; Harald Noedl; Sanjay K. Kochar; Dhanpat K. Kochar; Srivicha Krudsood; Marcus V. Lacerda; Alejandro Llanos-Cuentas; Ronnatrai Rueangweerayut; Ramadurai Srinivasan; Moritz Treiber; Jörg J. Möhrle; Justin Green; Universidad del Valle, Cali; GlaxoSmithKline plc.; Sri Ramachandra Medical College & Research Institute (Deemed University); Shoklo Malaria Research Unit; MV Hospital and Research Centre; Medizinische Universitat Wien; Sardar Patel Medical College; University of Rajasthan; Mahidol University; Fundacao de Medicina Tropical do Amazonas; Universidad Peruana Cayetano Heredia; Mae Sot General Hospital; Clinical Development
    Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 Plasmodium vivax-infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05-20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18-25°C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas.