Browsing by Author "Jaruwan Khaoplab"

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    Latticed gold nanoparticle conjugation via monomeric streptavidin in lateral flow assay for detection of autoantibody to interferon-gamma
    (2021-06-01) Weeraya Thongkum; Umpa Yasamut; Koollawat Chupradit; Supachai Sakkhachornphop; Jiraprapa Wipasa; Kanokporn Sornsuwan; On Anong Juntit; Rawiwan Pornprasit; Wanwisa Thongkamwitoon; Jirapan Chaichanan; Jaruwan Khaoplab; Chonnikarn Chanpradab; Watchara Kasinrerk; Chatchai Tayapiwatana; Mahidol University; Chiang Mai University
    Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was cre-ated. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptav-idin (6HISMBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.