Publication: Latticed gold nanoparticle conjugation via monomeric streptavidin in lateral flow assay for detection of autoantibody to interferon-gamma
Issued Date
2021-06-01
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20754418
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2-s2.0-85107827988
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Mahidol University
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SCOPUS
Bibliographic Citation
Diagnostics. Vol.11, No.6 (2021)
Suggested Citation
Weeraya Thongkum, Umpa Yasamut, Koollawat Chupradit, Supachai Sakkhachornphop, Jiraprapa Wipasa, Kanokporn Sornsuwan, On Anong Juntit, Rawiwan Pornprasit, Wanwisa Thongkamwitoon, Jirapan Chaichanan, Jaruwan Khaoplab, Chonnikarn Chanpradab, Watchara Kasinrerk, Chatchai Tayapiwatana Latticed gold nanoparticle conjugation via monomeric streptavidin in lateral flow assay for detection of autoantibody to interferon-gamma. Diagnostics. Vol.11, No.6 (2021). doi:10.3390/diagnostics11060987 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76156
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Title
Latticed gold nanoparticle conjugation via monomeric streptavidin in lateral flow assay for detection of autoantibody to interferon-gamma
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Abstract
Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was cre-ated. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptav-idin (6HISMBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.