Scopus 2021
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Publication Metadata only The calming effect of roasted coffee aroma in patients undergoing dental procedures(2021-12-01) Praewpat Pachimsawat; Kanlayanee Tangprasert; Nattinee Jantaratnotai; Mahidol UniversityCoffee beverage consumption is well-known to exert various health benefits; however, the effects of coffee aroma are rarely explored. This study aimed to investigate the calming effect of inhaling coffee aroma while the patients underwent dental procedures (probing and scaling). Salivary α-amylase (sAA) and cortisol (sCort) levels were measured as proxies of sympathetic nervous system and hypothalamic–pituitary–adrenal axis responses to stress respectively. Blood pressures and pulse rates were recorded. The results showed that undergoing dental procedures could increase sAA and sCort levels of the patients inhaling sham aroma while those inhaling coffee aroma had significantly decreased sAA and sCort levels (40% and 25% differences, respectively). The pulse rates of those inhaling coffee aroma were also lower. Subjective assessment using visual analog scale was in line with objective measures as well. The preference for coffee aroma or the frequency of coffee drinking had no effect on the sAA and sCort responses. This is the first study to provide evidence on the effect of coffee aroma on sAA and sCort levels in patients undergoing dental procedures.Publication Metadata only Optimization models for efficient (T, r) broadcast domination in graphs(2021-06-01) Poompol Buathong; Tipaluck Krityakierne; Mahidol University; Ministry of Higher Education, Science, Research and InnovationKnown to be NP-complete, domination number problems in graphs and networks arise in many real-life applications, ranging from the design of wireless sensor networks and biological networks to social networks. Initially introduced by Blessing et al., the (t, r) broadcast domination number is a generalization of the distance domination number. While some theoretical approaches have been addressed for small values of t, r in the literature; in this work, we propose an approach from an optimization point of view. First, the (t, r) broadcast domination number is formulated and solved using linear programming. The efficient broadcast, whose wasted signals are minimized, is then found by a genetic algorithm modified for a binary encoding. The developed method is illustrated with several grid graphs: regular, slant, and king’s grid graphs. The obtained computational results show that the method is able to find the exact (t, r) broadcast domination number, and locate an efficient broadcasting configuration for larger values of t, r than what can be provided from a theoretical basis. The proposed optimization approach thus helps overcome the limitations of existing theoretical approaches in graph theory.Publication Metadata only Implementing radical cure diagnostics for malaria: user perspectives on G6PD testing in Bangladesh(2021-12-01) Nora Engel; Cristian Ghergu; Mohammad Abdul Matin; Mohammad Golam Kibria; Kamala Thriemer; Ric N. Price; Xavier C. Ding; Rosalind E. Howes; Benedikt Ley; Sandra Incardona; Mohammad Shafiul Alam; Faculty of Tropical Medicine, Mahidol University; Foundation for Innovative New Diagnostics, Switzerland; Menzies School of Health Research; Universiteit Maastricht; International Centre for Diarrhoeal Disease Research Bangladesh; Nuffield Department of MedicineBackground: The radical cure of Plasmodium vivax requires treatment with an 8-aminoquinoline drug, such as primaquine and tafenoquine, to eradicate liver hypnozoite stages, which can reactivate to cause relapsing infections. Safe treatment regimens require prior screening of patients for glucose-6-phosphate dehydrogenase (G6PD) deficiency to avoid potential life-threatening drug induced haemolysis. Testing is rarely available in malaria endemic countries, but will be needed to support routine use of radical cure. This study investigates end-user perspectives in Bangladesh on the introduction of a quantitative G6PD test (SD Biosensor STANDARD™ G6PD analyser) to support malaria elimination. Methods: The perspectives of users on the SD Biosensor test were analysed using semi-structured interviews and focus group discussions with health care providers and malaria programme officers in Bangladesh. Key emerging themes regarding the feasibility of introducing this test into routine practice, including perceived barriers, were analysed. Results: In total 63 participants were interviewed. Participants emphasized the life-saving potential of the biosensor, but raised concerns including the impact of limited staff time, high workload and some technical aspects of the device. Participants highlighted that there are both too few and too many P. vivax patients to implement G6PD testing owing to challenges of funding, workload and complex testing infrastructure. Implementing the biosensor would require flexibility and improvisation to deal with remote sites, overcoming a low index of suspicion and mutual interplay of declining patient numbers and reluctance to test. This approach would generate new forms of evidence to justify introduction in policy and carefully consider questions of deployment given declining patient numbers. Conclusions: The results of the study show that, in an elimination context, the importance of malaria needs to be maintained for both policy makers and the affected communities, in this case by ensuring P. vivax, PQ treatment, and G6PD deficiency remain visible. Availability of new technologies, such as the biosensor, will fuel ongoing debates about priorities for allocating resources that must be adapted to a constantly evolving target. Technical and logistical concerns regarding the biosensor should be addressed by future product designs, adequate training, strengthened supply chains, and careful planning of communication, advocacy and staff interactions at all health system levels.Publication Metadata only Zika virus isolation, propagation, and quantification using multiple methods(2021-07-01) Worawat Dangsagul; Kriengsak Ruchusatsawat; Apiwat Tawatsin; Don Changsom; Pirom Noisumdaeng; Sukontip Putchakarn; Chayawat Phatihattakorn; Prasert Auewarakul; Pilaipan Puthavathana; Siriraj Hospital; Thailand Ministry of Public Health; Mahidol University; Thammasat UniversityZika virus (ZIKV) was isolated from the archival urine, serum, and autopsy specimens by intrathoracic inoculation of Toxorhynchitis splendens and followed by three blind sub-passaging in C6/36 mosquito cells. The virus isolates were identified using an immunofluorescence assay and real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). This study analyzed 11 ZIKV isolates. One isolate (0.6%) was obtained from 171 urine samples, eight (8.7%) from 92 serum samples and two from tissues of an abortive fetus. After propagation in C6/36 cells, ZIKV was titrated by plaque and focus forming unit (FFU) assays in Vero cell monolayers, and viral genomes were determined via real-time and digital RT-PCR. Plaque and FFU assay quantitations were comparable, with the amount of infectious viruses averaging 106−107 PFU or FFU/ml. Real-time RT-PCR semi-quantified the viral genome numbers, with Ct values varying from 12 to 14. Digital RT-PCR, which precisely determines the numbers of the viral genomes, consistently averaged 10-100 times higher than the number of infectious units. There was good correlation between the results of these titration methods. Therefore, the selection of a method should be based on the objectives of each research studies.Publication Metadata only The Effects of Modest Alcohol Consumption on Non-alcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis(2021-08-27) Wasit Wongtrakul; Sorachat Niltwat; Phunchai Charatcharoenwitthaya; Siriraj Hospital; Srinakharinwirot UniversityBackground and Objective: There is no consensus regarding modest alcohol consumption in patients with non-alcoholic fatty liver disease (NAFLD) due to conflicting results. The aim of this meta-analysis was to examine the effects of modest alcohol consumption on histological severity, histological course, hepatocellular carcinoma, and long-term clinical outcomes in NAFLD patients. Methods: We searched MEDLINE and EMBASE databases from inception to October 2020 for studies evaluating the effects of modest alcohol consumption among patients with NAFLD. A random-effects meta-analysis using pooled odds ratio (OR) and hazard ratio (HR) was calculated with 95% confidence interval (CI). Study quality was assessed with the Newcastle-Ottawa Scale. Results: Fourteen cross-sectional or cohort studies with aggregate data on 14,435 patients were included in the analysis. Modest alcohol consumption resulted in lower risks for steatohepatitis (OR 0.59; 95% CI 0.45–0.78; I2 = 12%) and advanced fibrosis (OR 0.59, 95% CI 0.36–0.95; I2 = 75%). Histological follow-up data showed that modest alcohol use was associated significantly with less steatohepatitis resolution but not with fibrosis progression. The HR for developing hepatocellular carcinoma was 3.77 (95% CI 1.75–8.15; I2 = 0%). NAFLD patients with modest alcohol intake had a lower mortality risk than lifelong abstainers (HR 0.85; 95% CI 0.75–0.95; I2 = 64%). Conclusion: This meta-analysis suggests that medical advice for modest alcohol drinking should be made cautiously in caring for an individual patient based on the clinical context. Practically, patients with steatohepatitis or advanced fibrosis should avoid alcohol use, whereas patients with low fibrosis risk may be allowed for modest and safe drinking.Publication Metadata only Effect of releasing quadratus lumborum muscle on hip and knee muscle length in asymptomatic individuals(2021-04-01) Yaowapa Jairakdee; Wunpen Chansirinukor; Tippawan Sitti; Mahidol UniversityBackground: Quadratus lumborum muscle (QL) is one of several muscles subject to tightness and relevant to symptoms in the back and hip. Although releasing the tight QL seems to resolve these symptoms in clinic, no study has investigated the effects of such releasing on the length of the hip and knee muscles. Objective: To compare muscle length of the hip and knee joints between pre- and post-releasing the QL. Methods: A quasi-experimental design (one-group pretest-posttest design) was conducted. Thirty asymptomatic participants with mean age of 20.40 years took part in the study. An examiner assessed the participants’ pelvic transverse gliding movement in standing and rotation of the upper trunk in supine to perceive the end feel of tissue resistance. The side with perceived more muscle tension was selected for receiving muscle release. Before manually releasing the QL, the participant was in the modified Thomas test position and pre-test range of motion (ROM) of hip flexion, hip abduction, and knee flexion angles was measured using a standard goniometer. After releasing the QL, the aforementioned angles were measured for post-test ROM. Results: The hip flexion angle was significantly reduced after releasing the QL (p < 0.05), whereas no statistically significant differences were found for the other 2 angles (p > 0.05). Conclusion: The length of iliopsoas muscle was increased after releasing the QL. The findings may be due to continuous fascial connection and similar attachment of the origins of these 2 muscles. Clinical trial registration number: NCT03016559.Publication Metadata only Buffering capacity and antibacterial properties among bioactive glass-containing orthodontic adhesives(2021-01-01) Tanawan Wanitwisutchai; Naruporn Monmaturapoj; Ratchapin Srisatjaluk; Kittitat Subannajui; Surachai Dechkunakorn; Niwat Anuwongnukroh; Pong Pongprueksa; Mahidol University, Faculty of Dentistry; Mahidol University; Thailand National Science and Technology Development AgencyThis study was to evaluate the acid-buffering capacity and antibacterial properties of orthodontic adhesives containing bioactive glasses (BAGs) (45S5, 45S5F, S53P4), Hydroxyapatite, beta-tricalcium phosphate, and Canasite. Fillers comprising 15 wt% bioactive glasses, HAp, β-TCP, and Canasite incorporated with 55 wt% silanated glass were added to a mixture of UDMA/TEGDMA. Acid-buffering capacity was tested by exposing disc-shaped samples of each adhesive to medium of bacteria-produced acids, and pH changes were recorded at 24 and 48 h. Antibacterial properties were assessed by indirect testing by exposing polymerized adhesive samples to a medium and direct testing by immersing the specimens in solutions containing S. mutans and S. sanguinis. A significant buffering capacity was shown by the 45S5, 45S5F and S53P4 BAG adhesives. The antibacterial properties were not significant in all experimental adhesives. Therefore, the experimental orthodontic adhesives containing BAGs demonstrated a significant buffering capacity but did not show significant antibacterial properties against S. mutans and S. sanguinis.Publication Metadata only Southeast Asia is an emerging hotspot for COVID-19(2021-09-01) Thanat Chookajorn; Theerarat Kochakarn; Chaiwat Wilasang; Namfon Kotanan; Charin Modchang; Faculty of Tropical Medicine, Mahidol University; Umeå Universitet; Mahidol University; Ministry of Higher Education, Science, Research and Innovation; COVID-19 Network Investigations AlliancePublication Metadata only Graves’ disease following SARS-CoV-2 vaccination(2021-12-01) Chutintorn Sriphrapradang; Prapimporn Ch Shantavasinkul; Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityPublication Metadata only Novel supergroup u wolbachia in bat mites of Thailand(2021-02-04) Phanthip Olanratmanee; Visut Baimai; Arunee Ahantarig; Wachareeporn Trinachartvanit; Rajabhat Rajanagarindra University; Mahidol University, Center of Excellence for Vectors and Vector-Borne Diseases; Mahidol UniversityWolbachia is an endosymbiont infecting mostly arthropods and is vertically transmitted to its host offspring; however, Wolbachia in mites has rarely been reported. We have discovered, for the first time, Wolbachia endosymbiont in Spinturnix mite infesting thick-thumbed myotis bat (Myotis rosseti) in Thailand. Wolbachia spp were detected in Spinturnix spp by PCR employing species-specific 16S rDNA and ftsZ primers. Consensus sequences were then used to construct phylogenetic trees, which indicated Spinturnix mite Wolbachia spp belonged to a clade separate from existing species, which we named Wolbachia Supergroup U. The role of Wolbachia spp in Spinturnix mites remains to be determined.Publication Metadata only Peptide barcodes in dogs affected by mitral valve disease with and without pulmonary hypertension using MALDI-TOF MS and LC-MS/MS(2021-08-01) Nattapon Riengvirodkij; Sittiruk Roytrakul; Janthima Jaresitthikunchai; Narumon Phaonakrop; Sawanya Charoenlappanich; Walasinee Sakcamduang; Mahidol University; Thailand National Center for Genetic Engineering and BiotechnologyMitral valve disease (MVD) is an important and most frequently acquired heart disease found in dogs. MVD is classified into different stages according to its severity. There is a challenge in differentiation between asymptomatic and symptomatic stages of the MVD. Moreover, pulmonary hypertension (PH) is a common complication in dogs affected by MVD. In clinical practice, there are also some limitations to identify PH. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a technique that can characterize specific patterns of peptide mass called peptide barcodes from various samples. Besides, in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS), potential peptide sequences associated with specific conditions could be identified. The present study aimed to use MALDI-TOF coupled with LC-MS/MS to characterize specific peptide barcodes and potential peptide candidates in serum samples from healthy dogs, dogs with MVD stage B (MVD B, asymptomatic stage), MVD stage C (MVD C, symptomatic stage), MVD stage B with PH (MVD B PH), and MVD stage C with PH (MVD C PH). Discrete clusters of the 5 sample groups were identified by 3D plot analysis. Peptide barcodes also revealed differences in peptide patterns among the 5 groups. Six amino acid sequences of peptide candidates at 1,225.60, 1,363.85, 1,688.71, 1789.52, 2020.21, and 2156.42 Da were identified as part of the proteins CLCN1, CLUL1, EDNRA, PTEN, SLC39A7, and CLN6, respectively. The network interactions between these discovered proteins and common cardiovascular drugs were also investigated. These results demonstrate that MALDI-TOF MS has promise as an optional technique for diagnosing dogs affected by asymptomatic and symptomatic stages of MVD with and without PH. Further studies are required to identify peptide barcodes in dogs with other diseases to create peptide barcode databases in veterinary medicine before using this method as a novel diagnostic tool in the future.Publication Metadata only Comprehensive morpho-histological observation of digestive system and gut content of wild-grunting toadfish, allenbatrachus grunniens (Linnaeus, 1758)(2021-09-01) Tappadit Mitparian; Sinlapachai Senarat; Piyakorn Boonyoung; Wannee Jiraungkoorskul; Gen Kaneko; Jes Kettratad; University of Houston-Victoria; Rajamangala University of Technology Srivijaya; Chulalongkorn University; Mahidol University; Prince of Songkla UniversityThe morphology, histology and histochemistry of the digestive system and gut content of the wild-grunting toadfish (Allenbatrachus grunniens) from the estuary of Pranburi River, Thailand, is investigated. The digestive system of A. grunniens is elongated from the oral cavity to the intestine except for the stomach of a sac-shaped type. The length of the elongated intestine has the intestine coefficient (IC) of 0.52 ± 0.1. The digestive tract is composed of four layers: mucosa, submucosa, muscularis and serosa. The longitudinal folds of the esophagus are lined by the stratified mucosal epithelium which contains several large secretory cells. The gastric rugae in both the cardiac and pyloric regions of the stomach are lined with a simple columnar epithelium. The intestine has numerous intestinal folds lined by simple columnar epithelia. Intestinal goblet cells are organised and inserted among the epithelia. The index of relative importance (IRI) suggests that the main food items are a crab group (73.64%) followed by shrimps (10.43%), fishes (8.02%) and other compositions (7.92%). The IC, morpho-histological features of the digestive system and IRI indicate that A. grunniens is a carnivorous fish.Publication Metadata only Protective effect of panduratin a on cisplatin-induced apoptosis of human renal proximal tubular cells and acute kidney injury in mice(2021-06-01) Penjai Thongnuanjan; Sirima Soodvilai; Somsak Fongsupa; Napason Chabang; Pornpun Vivithanaporn; Patoomratana Tuchinda; Sunhapas Soodvilai; Rangsit University; Faculty of Medicine Ramathibodi Hospital, Mahidol University; Mahidol University; Thammasat UniversityBackground: Cisplatin is an effective chemotherapy but its main side effect, acute kidney injury, limits its use. Panduratin A, a bioactive compound extracted from Boesenbergia rotunda, shows several biological activities such as anti-oxidative effects. The present study investigated the nephroprotective effect of panduratin A on cisplatin-induced renal injury. Methods: We investigated the effect of panduratin A on the toxicity of cisplatin in both mice and human renal cell cultures using RPTEC/TERT1 cells. Results: The results demonstrated that panduratin A ameliorates cisplatin-induced renal toxicity in both mice and RPTEC/ TERT1 cells by reducing apoptosis. Mice treated with a single intraperitoneal (i.p.) injection of cisplatin (20mg/kg body weight (BW)) exhibited renal tubule injury and impaired kidney function as shown by histological examination and increased serum creatinine. Co-administration of panduratin A (50mg/kg BW) orally improved kidney function and ameliorated renal tubule injury of cisplatin by inhibiting activation of extracellular signal-regulated kinase (ERK)1/2 and caspase 3. In human renal proximal tubular cells, cisplatin induced cell apoptosis by activating pro-apoptotic proteins (ERK1/2 and caspase 3), and reducing the anti-apoptotic protein (Bcl-2). These effects were significantly ameliorated by co-treatment with panduratin A. Interestingly, panduratin A did not alter intracellular accumulation of cisplatin. It did not alter the anticancer efficacy of cisplatin in either human colon or non-small cell lung cancer cell lines. Conclusions: The present study highlights panduratin A has a potential protective effect on cisplatin’s nephrotoxicity.Publication Metadata only Towards Self-Regulated Individual Learning Path Generation Using Outcome Taxonomies and Constructive Alignment(2021-01-01) Phat Nguyen Huu; Preecha Tangworakitthaworn; Lester Gilbert; University of Southampton; Mahidol UniversitySelf-regulated individual learning is widely used in academia. Besides the model's advantages, such as flexible learning in time and space, some implementations have limitations, for example fixed learning paths, and unclear relationships between learning activities and intended learning outcomes. This paper introduces an individualized learning model based on Bloom's cognitive taxonomy and Biggs' Principle of Constructive Alignment (PCA). The model provides individual tailored learning paths, adjusted for different background knowledge and ability to learn, based on regularly measured achievement of the intended learning outcomes.Publication Metadata only Association of infant feeding practices with iron status and hematologic parameters in 6-month-old infants(2021-12-01) Chayatat Ruangkit; Nawapat Prachakittikul; Nutthida Hemprachitchai; Oraporn Dumrongwongsiri; Sasivimon Soonsawad; Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityBackground: Infants’ feeding practices in the first 6 months of life and their association with iron status and hematologic parameters has not been well studied. We aim to evaluate this association. Methods: In a retrospective chart review, we identified 403 infants who received laboratory screening for anemia at 6-month visits. Infants were categorized into four groups according to feeding practices. Hematologic parameters and incidence of anemia, iron deficiency (ID), and iron deficiency anemia (IDA) were compared. Results: In total, 105 infants were breastfed (BF), 78 were breastfed with iron supplementation starting at 4 months (BI), 109 were mixed-fed (breast milk and formula) with or without iron supplementation (MF), and 111 were formula-fed (FF). The BF group had the highest incidence of anemia (38.1%), ID (28.6%), and IDA (17.1%) when compared with the other groups (p < 0.001). In multivariate logistic regression, BI, MF, and FF infants had 90.4%, 97.5%, and 96.9% decreased risk of IDA, respectively, with BF infants as a reference group. Conclusion: The incidence of anemia, ID, and IDA at age 6 months was higher in BF than FF or MF infants. However, iron supplements in BF infants starting at 4 months significantly reduced their ID and IDA incidence.Publication Metadata only Dental tutorbot: Exploitation of dental textbooks for automated learning(2021-01-01) Fizza Tauqeer; Muhammad Khubaib Raza; Myat Su Yin; Saeed Ul Hassan; Peter Haddawy; Mihai Pomarlan; Suppawong Tuarob; Information Technology University; Mahidol University; Universität BremenActive learning has been shown to provide benefits over traditional didactic approaches to teaching and learning. These benefits are particularly important in fields in which students must master large amounts of information and effectively operationalize it, as in medicine and dentistry. While online learning platforms have the potential to provide students with active learning without taxing scarce faculty resources, a recognized challenge in producing such systems is the engineering of the domain knowledge needed for engaging interaction. In this paper we address this problem by developing an open-source chatbot-based tutoring system trained on dental textbooks in the area of endodontics, one of the most challenging areas of dentistry. Dental TutorBot is built using Rasa for modular training purposes. It asks short questions from students and evaluates their answers. If the student cannot answer a question, the system provides a hint, rather than immediately giving the student the answer. In this way, it coaches the student to find the answer and thus helps them to understand the connections between concepts while creating a more intellectually stimulating learning experience.Publication Metadata only A novel nucleic lateral flow assay for screening phaR-containing Bacillus spp.(2021-01-28) Nay Yee Wint; Khine Kyi Han; Wariya Yamprayoonswat; Pattarawan Ruangsuj; Supachoke Mangmool; Chamras Promptmas; Montri Yasawong; Chulabhorn Royal Academy; Mahidol University; Center of Excellence on Environmental Health and Toxicology (EHT)Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that contain a class IV PHA synthase, such as some Bacillus spp. The phaR gene was developed to screen phaR-containing Bacillus spp. The phaR screening method involved two steps: phaR gene amplification by PCR and phaR amplicon detection using a DNA lateral flow assay. The screening method has a high specificity for phaR-containing Bacillus spp. The lowest amount of genomic DNA of B. thuringiensis ATCC 10792 that the phaR screening method could detect was 10 pg. This novel screening method improves the specificity and sensitivity of phaR gene screening and reduces the time and cost of the screening process, which could enhance the opportunity to discover good candidate PHA producers. Nevertheless, the screening method can certainly be used as a tool to screen phaR-containing Bacillus spp. from environmental samples.Publication Metadata only In vitro characterization of chrysovirus-1-induced hypovirulence of bipolaris maydis(2021-01-01) Passanesh Sukphopetch; San Suwanmanee; Potjaman Pumeesat; Sumate Ampawong; Bansomdejchaopraya Rajabhat University; Mahidol UniversityMycoviruses are viruses that infect fungi. Chrysovirus-1 has been reported as a mycovirus in Bipolaris maydis, which is the plant fungal pathogen for maize, and its infection results in the reduction of crop yield. We aimed to characterize Chrysovirus-1-infected B. maydis in terms of macroscopic morphology, transmission electron microscopy, colony size, biofilm formation, and stress responses focusing on osmotic stress (NaCl), oxidative stress (H2O2), thermotolerance (at 25, 37, and 45 °C), pH (5, 7, and 10), and metal stress (ZnSO4) in comparison with an uninfected strain. Our results demonstrated the presence of viral-like particles under TEM. The colony morphology of the infected strain displayed slight differences as significant delay in colony growth of the Chrysovirus-1-infected strain when compared to the uninfected strain. Moreover, biofilm mass of the infected strain was examined as being lower than that of the uninfected strain. Several stress response tests also demonstrated that the infected strain exhibited higher sensitivity to all stress responses compared to the uninfected strain. Thus, our results suggested that mycoviruses as demonstrated in this study (Chrysovirus-1) can induce the hypovirulence phenomenon in the pathogenic fungi (B. maydis).Publication Metadata only Perception of contraceptive counseling and contraceptive use among systemic lupus erythematosus patients(2021-01-01) Pongkarn Buasawat; Jittima Manonai; Pintip Ngamjanyaporn; Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityObjectives: (1) To evaluate the perception of contraceptive counseling and knowledge regarding the impact of unintended pregnancy and (2) examine the prevalence of, and predictors for contraceptive use among systemic lupus erythematosus (SLE) patients. Study design: A cross-sectional survey was conducted at the Rheumatology Clinic of a university hospital. SLE patients who attended the clinic from April 2018 to January 2019 and at risk for unintended pregnancy and were invited to be interviewed. The structured interview guide consisted of three parts: personal and obstetric information, perception of contraceptive counseling and knowledge about the impact of unintended pregnancy, and contraceptive use. Results: The mean age of the 137 patients who gave informed consents was 34 ± 8 years, with 47 patients (34%) stating that they had received contraceptive counseling. Seventy-five patients (52%) reported practicing contraception. The intention to not become pregnant (Odds Ratio; 95% CI = 7.28; 1.75–30.27), perception of adequate contraceptive counseling (2.77; 1.06–7.20) and discussion with partners and physicians about contraception (3.95; 1.64–9.50) were predictors for contraceptive use (p < 0.05). Conclusion: Most patients reported no contraceptive counseling with approximately half of the patients using contraceptive methods within the past three months. Desire to avoid pregnancy, perception of adequate contraceptive counseling, and the influence of partners and physicians were significant predictors for contraceptive use. Implications: Contraceptive counseling should be routinely included in discussions about disease processes and treatment plans, including access issues. Best practices for contraceptive counseling and integrated, multi-disciplinary team services should be developed and provided in clinical settings.Publication Metadata only Heterogeneity of Helicobacter pylori bab genotypes and their association with clinical outcomes in Korean gastroduodenal patients(2021-07-01) Wongwarut Boonyanugomol; Kamolchanok Rukseree; Seung Chul Baik; Myunghwan Jung; Min Kyoung Shin; Hyung Lyun Kang; Woo Kon Lee; Mahidol University; Gyeongsang National University (GSNU), College of MedicineIn this study, we aimed to investigate the prevalence of bab genes (babA, babB, babC) at their three loci (loci A, B, and C) in Helicobacter pylori strains from varied clinical manifestations of Korean gastroduodenal patients. The overall prevalence of H. pylori Korean strains positive for babA and babB was 91.1% and 92.2%, respectively, but all strains were negative for bab C. H. pylori strains with two loci occupied (loci A and B) were the most prevalent in Korean patients (85.6%), compared to one locus occupied (14.4%) (locus A or B). Twelve bab genotypes were detected, additionally, the distribution of three bab genotypes was significantly associated with different clinical outcomes among Korean patients. The genotypes babA/babB/- and babA/babA were significantly associated with peptic ulcer disease (PUD) (63.3%) and gastritis (GT) (33.3%) patients, respectively. In addition, we found that the babA genotype was significantly associated with gastric cancer (GC) (36.7%) as compared to GT (6.7%) or PUD (6.7%) (p<0.05) patients. This study provided evidence that the bab genotypes in H. pylori Korean strains were highly variable. Interestingly, three patterns of bab genotypes were significantly different among patients with different clinical outcomes in the population at high-risk for GC.