Browsing by Author "Kevalin Vongthoung"
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Publication Metadata only Benjakul supplementation improves hepatic fat metabolism in high-fat diet-induced obese rats(2020-04-01) Achiraya Kamchansuppasin; Kevalin Vongthoung; Pornthep Temrangsee; Narongsuk Munkong; Nusiri Lerdvuthisopon; University of Phayao; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Thammasat University© Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. Purpose: To evaluate the effects of Benjakul water extract (BWE) supplementation for the prevention of hepatic fat metabolic dysfunction in a rat obesity model induced by a high-fat diet (HFD). Methods: Forty male outbred Sprague-Dawley rats were separated into six groups according to diet composition and treatment: control, HFD, and HFD supplemented with Benjakul extraction at low and high dose (41.3 and 413 mg/kg/day, respectively). After 4 weeks, blood biochemical parameters (i.e., hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) enzyme and liver histological features) were examined. Subsequently, hepatic gene expression of sterol regulatory element binding protein-1 (SREBP-1) and nuclear factor-kappa B (NF-kB) were investigated. Results: Low and high doses of BWE showed significant prevention of abdominal fat accumulation (p < 0.05) and inhibited hypercholesterolemia without restoring triglyceride (TG) and lipoprotein-cholesterol (LDL-C) in serum compared to rats fed HFD alone. BWE hindered hepatic fat accumulation via suppression of SREBP-1 expression and HMGCR activity in HFD-induced obese rats, while significantly promoting NF-kB down regulation (p < 0.05). Conclusion: BWE may be a novel prophylactic strategy for preventing metabolic syndrome and to protect against steatosis due to its regulatory effects on lipid homeostasis.Publication Metadata only Characterization of atypical lymphocytes and immunophenotypes of lymphocytes in patients with dengue virus infection(2007-03-01) Wipawee Jampangern; Kevalin Vongthoung; Akanitt Jittmittraphap; Surapon Worapongpaiboon; Kriengsak Limkittikul; Ampaiwan Chuansumrit; Uraiwan Tarunotai; Manas Chongsa-nguan; Mahidol University; Vajira HospitalTo characterize the immunophenotypes of lymphocytes in patients with dengue infection, we performed flow cytometric analysis of peripheral blood mononuclear cells collected from 49 dengue hemorrhagic fever (DHF), 25 dengue fever (DF), and 26 dengue-like syndrome (DLS) cases. The mean total atypical lymphocytes in DHF (916.1 ± 685.6 cells/ μl) and DF (876.2 ± 801.9 cells/μl) were higher than those of DLS (310.5 ± 181.4 cells/μl). An atypical lymphocyte count of 10% or higher was a good indicator of dengue infection (sensitivity 50% and specificity 86%). Flow cytometric studies showed that the percentages of atypical lymphocytes correlated with those of CD19+B lymphocytes and inversely correlated with the percentages of CD69+lymphocytes. The mean absolute counts of atypical lymphocytes and CD19+cells on the discharge day were significantly higher than those on the admission day. Low percentages of TdT+cells were found in all groups of patients. We concluded that atypical lymphocyte and CD19+cell counts may be a useful diagnostic tool for dengue infection and the recovery from the disease could be judged when numbers of both cell types are significantly elevated.Item Metadata only Characterization of the atypical lymphocyte in dengue hemorrhagic fever compared with dengue-like syndrome(2004) Wipawee Usawattanakul; วิภาวี อุษาวัฒนากูล; Kevalin Vongthoung; Akanitt Jittmittraphap; อกนิษฐ์ จิตต์มิตรภาพ; Kriengsak Limkittikul; เกรียงศักดิ์ ลิมป์กิตติกุล; Surapon Worapongpaiboon; Ampaiwan Chuansumrit; Uraiwan Tarunotai; Mahidol University. Faculty of Tropical medicineThe blood samples from 49 DHF, 25 DF, and 26 DLS were used to demonstrate atypical lymphocyte count and cellular immune activation, and to evaluate the proportion of CD4+, CD8+, CD19+, CD69+, CD87+ and TDT+ cells during the acute phase of dengue illness, by flow cytometry. The statistical methods used were the ttest, ANOVA, Pearson’s correlation test and the receiver operating characteristic (ROC) curve. The results revealed that the mean number of total atypical lymphocytes was higher in patients with DHF (916.1±685.6 cells/µl) than in those with DLS (310.5±181.4 cells/µl, p<0.05), and patients with DF had higher atypical lymphocytes (876.2±801.9 cells/µl) than DLS (310.5±181.4 cells/µl, p<0.05), but there were no differences between counts in patients with DHF and DF. In a ROC plot of the atypical lymphocyte, the selected cut-off value was 10 because it gave good sensitivity and specificity (50% and 86%). The mean percentage of CD4+ T cells was significantly reduced in DHF patients, but not significantly different in DHF patients compared with DF and DLS patients. The mean absolute count of CD19+ B cells in DHF (615±577 cells/µl) and DF (478±369 cells/µl) was significantly higher than DLS (246±169 cells/µl, p<0.05). A CD19 count of e” 15% or 20% could distinguish DF/DHF from DLS, with a sensitivity of 69% and specificity of 50% if the CD19 was e” 15%, and a sensitivity of 49% and specificity of 85% if the CD19 was e” 20%. The atypical lymphocytes, CD19+ B lymphocytes and CD69+ lymphocytes had significant correlation coefficientsr=0.403 and –0.241, respectively. The atypical lymphocyte had a linear positive correlation with the CD19+ B lymphocyte and a negative correlation with the CD69+ lymphocyte, at á=0.01. The percent of cells expressing CD69 was more increased on CD8+ T cells than CD4+ T cells, and CD19+ B cells, respectively. However the absolute count of CD69 expressed on all lymphocytes was higher than the absolute count of CD69 on total B and T lymphocytes. The atypical lymphocyte cell could not be differentiated from the lymphocyte by morphology only, but perhaps from NK cells. Further studies are needed to elucidate the roles of NK cells in the pathogenesis of dengue viral infection.