Publication: Characterization of atypical lymphocytes and immunophenotypes of lymphocytes in patients with dengue virus infection
3
Issued Date
2007-03-01
Resource Type
ISSN
0125877X
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2-s2.0-34250861206
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.25, No.1 (2007), 27-36
Suggested Citation
Wipawee Jampangern, Kevalin Vongthoung, Akanitt Jittmittraphap, Surapon Worapongpaiboon, Kriengsak Limkittikul, Ampaiwan Chuansumrit, Uraiwan Tarunotai, Manas Chongsa-nguan Characterization of atypical lymphocytes and immunophenotypes of lymphocytes in patients with dengue virus infection. Asian Pacific Journal of Allergy and Immunology. Vol.25, No.1 (2007), 27-36. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/24573
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Title
Characterization of atypical lymphocytes and immunophenotypes of lymphocytes in patients with dengue virus infection
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Abstract
To characterize the immunophenotypes of lymphocytes in patients with dengue infection, we performed flow cytometric analysis of peripheral blood mononuclear cells collected from 49 dengue hemorrhagic fever (DHF), 25 dengue fever (DF), and 26 dengue-like syndrome (DLS) cases. The mean total atypical lymphocytes in DHF (916.1 ± 685.6 cells/ μl) and DF (876.2 ± 801.9 cells/μl) were higher than those of DLS (310.5 ± 181.4 cells/μl). An atypical lymphocyte count of 10% or higher was a good indicator of dengue infection (sensitivity 50% and specificity 86%). Flow cytometric studies showed that the percentages of atypical lymphocytes correlated with those of CD19+B lymphocytes and inversely correlated with the percentages of CD69+lymphocytes. The mean absolute counts of atypical lymphocytes and CD19+cells on the discharge day were significantly higher than those on the admission day. Low percentages of TdT+cells were found in all groups of patients. We concluded that atypical lymphocyte and CD19+cell counts may be a useful diagnostic tool for dengue infection and the recovery from the disease could be judged when numbers of both cell types are significantly elevated.