Browsing by Author "Paula Gomes"

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    Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract
    (2013-12-01) Nuno Vale; Maria João Gouveia; Mónica Botelho; Banchob Sripa; Sutas Suttiprapa; Gabriel Rinaldi; Paula Gomes; Paul J. Brindley; José Manuel Correia da Costa; Universidade do Porto; National Health Institute Doutor Ricardo Jorge Porto; Khon Kaen University; Mahidol University; George Washington University; Universidad de la Republica Facultad de Medicina
    Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone. © 2013 Published by Elsevier Ireland Ltd.