Publication: Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract
Issued Date
2013-12-01
Resource Type
ISSN
18730329
13835769
13835769
Other identifier(s)
2-s2.0-84883274221
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Parasitology International. Vol.62, No.6 (2013), 535-542
Suggested Citation
Nuno Vale, Maria João Gouveia, Mónica Botelho, Banchob Sripa, Sutas Suttiprapa, Gabriel Rinaldi, Paula Gomes, Paul J. Brindley, José Manuel Correia da Costa Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract. Parasitology International. Vol.62, No.6 (2013), 535-542. doi:10.1016/j.parint.2013.08.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31831
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract
Abstract
Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone. © 2013 Published by Elsevier Ireland Ltd.