Browsing by Author "Raju Kucherlapati"

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    Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system
    (2005-03-01) Michelle Dziejman; Davide Serruto; Vincent C. Tam; Derek Sturtevant; Pornphan Diraphat; Shah M. Faruque; M. Hasibur Rahman; John F. Heidelberg; Jeremy Decker; Li Li; Kate T. Montgomery; George Grills; Raju Kucherlapati; John J. Mekalanos; Harvard Medical School; International Centre for Diarrhoeal Disease Research Bangladesh; J. Craig Venter Institute; Harvard Partners Genome Center; Brigham and Women's Hospital; University of Rochester School of Medicine and Dentistry; Novartis Immunobiological Research Institute; Mahidol University
    Non-O1, non-O139 Vibrio cholerae can cause gastroenteritis and extraintestinal infections, but, unlike O1 and O139 strains of V. cholerae, little is known about the virulence gene content of non-O1, non-O139 strains and their phylogenetic relationship to other pathogenic V. cholerae. Comparative genomic microarray analysis of four pathogenic non-O1, non-O139 strains indicates that these strains are quite divergent from O1 and O139 strains. Genomic sequence analysis of a non-O1, non-O139 strain (AM-19226) that appeared particularly pathogenic in experimental animals suggests that this strain carries a type III secretion system (TTSS) that is related to the TTSS2 gene cluster found in a pandemic clone of Vibrio parahaemolyticus. The genes for this V. cholerae TTSS system appear to be present in many clinical and environmental non-O1, non-O139 strains, including at least one clone that is globally distributed. We hypothesize that the TTSS present in some pathogenic strains of non-O1, non-O139 V. cholerae may be involved in the virulence and environmental fitness of these strains. © 2005 by The National Academy of Sciences of the USA.
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    Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system
    (2005-03-01) Michelle Dziejman; Davide Serruto; Vincent C. Tam; Derek Sturtevant; Pornphan Diraphat; Shah M. Faruque; M. Hasibur Rahman; John F. Heidelberg; Jeremy Decker; Li Li; Kate T. Montgomery; George Grills; Raju Kucherlapati; John J. Mekalanos; Mahidol university. Faculty of Public Health
    Non-O1, non-O139 Vibrio cholerae can cause gastroenteritis and extraintestinal infections, but, unlike O1 and O139 strains of V. cholerae, little is known about the virulence gene content of non-O1, non-O139 strains and their phylogenetic relationship to other pathogenic V. cholerae. Comparative genomic microarray analysis of four pathogenic non-O1, non-O139 strains indicates that these strains are quite divergent from O1 and O139 strains. Genomic sequence analysis of a non-O1, non-O139 strain (AM-19226) that appeared particularly pathogenic in experimental animals suggests that this strain carries a type III secretion system (TTSS) that is related to the TTSS2 gene cluster found in a pandemic clone of Vibrio parahaemolyticus. The genes for this V. cholerae TTSS system appear to be present in many clinical and environmental non-O1, non- O139 strains, including at least one clone that is globally distributed. We hypothesize that the TTSS present in some pathogenic strains of non-O1, non-O139 V. cholerae may be involved in the virulence and environmental fitness of these strains.