Browsing by Author "Rungsipipat A."

Filter results by typing the first few letters
Now showing 1 - 5 of 5
  • No Thumbnail Available
    ItemMetadata only
    Case report: Mature extragonadal teratoma at the proximal part of the tail in a kitten
    (2022-11-21) Sirivisoot S.; Siripara N.; Arya N.; Techangamsuwan S.; Rungsipipat A.; Kasantikul T.; Mahidol University
    An 8-month-old, intact male, domestic shorthair cat was referred for a mass on the proximal ventral part of the tail which had been found since the animal was born, and due to the presence of a linear fissure with rows of ectopic teeth, the veterinarian suspected that the mass had recently ruptured. Tail amputation was elected and the entire mass was successfully surgically excised. From the gross examination, this mass had an open cyst-like structure with a prominent area composed of hair, teeth, and bone. Histopathology revealed two components of germinal layers including hair follicles, adnexal tissue, neural tissue, teeth, muscle, fat, bone, and lymphatic vessels. The histopathological diagnosis was consistent to mature teratoma. Although, complete excision could not be definitively confirmed histologically, this kitten is currently well and has not developed any recurrent mass at the surgical site after 2 years of post-operation.
  • No Thumbnail Available
    ItemMetadata only
    Comparative compositional and functional venomic profiles among venom specimens from juvenile, subadult and adult Russell’s viper (Daboia siamensis): correlation with renal pathophysiology in experimental rabbits
    (2022-01-01) Chaiyabutr N.; Chanhome L.; Vasaruchapong T.; Laoungbua P.; Khow O.; Rungsipipat A.; Reamtong O.; Sitprija V.; Mahidol University
    Background: Eastern Russell’s viper (Daboia siamensis) is one of the most medically significant snakes responsible for the development of acute renal failure. However, variation of the clinical picture and renal pathophysiology following bites by young and adult D. siamensis have not been elucidated. Methods: In this study, we analyzed the venomic profiles of D. siamensis at different maturation stages of juvenile, subadult and adult groups. The same pooled venom from each group was subjected to enzymatic, electrophoretic and proteomic analysis, including sublethal toxicity (0.1 mg/kg iv.) examined on bodily functions by comparing the venom compositional and functional profiles among venom specimens from juvenile, subadult and adult D. siamensis by correlating them with the renal pathophysiology in experimental rabbits. Results: The comparative studies revealed that juvenile venom possessed higher phospholipase A2, metalloproteinase and serine proteinase levels, while subadult and adult venoms contained more L-amino acid oxidase, phosphodiesterase, the Kunitz-type serine protease inhibitor, disintegrin families and endothelial growth factor. An in vivo study revealed that the adult and subadult venoms caused persistent hypotension and bradycardia, while thrombocytopenia was a more characteristic effect of juvenile venom. All venom age groups showed significant reductions in renal hemodynamics and electrolyte excretions. The juvenile venom caused a higher tubulonephrosis lesion score than adult and subadult venoms. Conclusions: The D. siamensis venom shows an ontogenetic shift in its compositions and activities. Renal function alterations after envenomation depend on either the synergistic actions of different venom components or the disproportionate expression between the concentrations of enzymatic and non-enzymatic proteins in each age venom group. The high proportion of enzymatic toxin proteins in the juvenile venom results in greater nephrotoxicity.
  • No Thumbnail Available
    ItemMetadata only
    Comparative E-cadherin and syndecan-1 protein expression in human and canine oral squamous cell carcinoma
    (2024-01-09) Tabtieang S.P.; Paphussaro W.; Rungsipipat A.; Kunnasut N.; Ploypetch S.; Phattarataratip E.; Suriyaphol G.; Tabtieang S.P.; Mahidol University
    Oral squamous cell carcinoma (OSCC) is a prevalent form of oral cancer in humans and dogs. The altered expression of cell adhesion molecules, including E-cadherin (CDH1) and syndecan-1 (SDC1), is involved in cancer progression. This study aimed to investigate the protein expression of CDH1 and SDC1 in early and late clinical stages of human and canine OSCC (hOSCC and cOSCC, respectively), using immunohistochemistry. Formalin-fixed and paraffin embedded tissue blocks were obtained from 21 hOSCC, 8 human normal gingiva, 26 cOSCC, and 13 canine normal gingiva. Clinical stages and histological subtypes of samples were evaluated. The results indicated that both human and canine OSCC exhibited reduced levels of CDH1 and SDC1 expression at the cell membrane regardless of clinical stage or histological subtype. Additionally, decreased levels of total SDC1 expression were observed in hOSCC compared with normal controls. In conclusion, this study demonstrates a similarity in the immunohistochemical expression of CDH1 and SDC1 between humans and dogs with OSCC, lending support to the potential use of dogs as a model for studying human head and neck squamous cell carcinoma.
  • No Thumbnail Available
    ItemMetadata only
    Ivabradine Preserves Cardiac Structure and Reduces Cell Death in Four Dogs With Preclinical Mitral Valve Disease
    (2026-01-01) Pirintr P.; Limprasutr V.; Rungsipipat A.; Rattanapinyopituk K.; Sawangkoon S.; Saengklub N.; Kijtawornrat A.; Pirintr P.; Mahidol University
    BACKGROUND: Ivabradine, a specific funny channel blocker, has shown cardiovascular benefits in humans and animals. However, its effects on haemodynamics, cardiac function, ventricular remodelling and cardiac apoptosis in asymptomatic dogs with myxomatous mitral valve disease (MMVD) stage B2 remain unclear. OBJECTIVE: To investigate the effect of long-term oral ivabradine on heart rate (HR), haemodynamics, cardiac function and apoptosis in dogs with MMVD stage B2. METHODS: Four dogs with MMVD stage B2 received ivabradine (1 mg/kg orally, q12h) for 3 months. Under general anaesthesia (propofol and isoflurane), electrocardiograms and invasive measurements of left ventricular, aortic, pulmonic, right atrial and pulmonary capillary wedge pressures were obtained at baseline and 3 months. Endomyocardial biopsies were collected at both timepoints for histopathology and immunohistochemical evaluation of Bcl-2 and Bax expression to assess apoptosis. RESULTS: Ivabradine decreased HR by 30.4% (p < 0.05) without significantly affecting systemic or pulmonary pressures, vascular resistance or systolic/diastolic function. Histopathological analysis showed significant reductions in myocyte vacuolization (68.5%), glycogen accumulation (47.6%), interstitial fibrosis (57.3%) and fibrofatty infiltration (77.8%) (p < 0.05). The apoptosis index was reduced by 17.0% (p < 0.05), and Bcl-2 expression tended to increase (p = 0.06). CONCLUSION: Chronic ivabradine treatment lowered HR and reduced cardiac structural remodelling and apoptosis without impairing cardiac function in dogs with MMVD stage B2. These findings support the potential of ivabradine as a therapeutic option for asymptomatic canine heart disease.
  • No Thumbnail Available
    ItemMetadata only
    Renal epitheliotropism of feline morbillivirus in two cats
    (2022-01-01) Chaiyasak S.; Piewbang C.; Yostawonkul J.; Boonrungsiman S.; Kasantikul T.; Rungsipipat A.; Techangamsuwan S.; Mahidol University
    The association of feline morbillivirus (FeMV) with kidney disease in cats is controversial. Two cats with a history of severe hematuria had eosinophilic inclusion-like bodies in the renal tubular epithelial cells, without any inflammatory cellular reaction. Ultrastructurally, aggregations of electron-dense viral-like particles were found where the inclusion-like bodies were located. Immunohistochemistry (IHC) using antibodies against FeMV matrix protein labeled these inclusion-like bodies, and also labeled the cytoplasm of tracheal and bronchiolar epithelial cells, and lymphocytes and macrophages in spleen and mesenteric lymph node. Using double IHC, FeMV antigen was detected in astroglia and oligodendroglia but not in microglia. Phylogenetic characterization of the fusion and hemagglutinin gene sequences revealed FeMV-1A genotypes in both cats. These findings indicated an active viral infection with FeMV. We propose that FeMV is a renal epitheliotropic virus and also localizes in various other tissues.