Browsing by Author "Sangloung, C."

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    In vivo culture of cryptosporidium oocysts for laboratory use.
    (2006) Sangloung, C.; Buddhirongawatr, R.; Tantawiwattananon, N.; Sukthana, Y.; Charoonluk Sangloung.; Nitipan Tantawiwattananon.; Ruangrat Buddhirongawatr.; Yaowalark Sukthana.; Mahidol University. International College. Planning Research and Development Department.
    In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,00120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4°C before use.