Browsing by Author "Sei ichi Aiba"

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    Production of Polyhydroxyalkanoates from Crude Glycerol Using Recombinant Escherichia coli
    (2015-01-01) Chitwadee Phithakrotchanakoon; Verawat Champreda; Sei ichi Aiba; Kusol Pootanakit; Sutipa Tanapongpipat; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; National Institute of Advanced Industrial Science and Technology
    © 2014, Springer Science+Business Media New York. The high production cost of bio-based plastic polyhydroxyalkanoates (PHAs) limits their use as commercial products. Thus, systems for PHAs production from waste substrates could reduce production costs. Crude glycerol is a by-product of biodiesel fuel production and thus represents an inexpensive, abundant and promising carbon source for production of valorized fermentation products. In this study, industrial crude glycerol by-product from palm oil biodiesel production was used as the carbon source for production of PHAs by recombinant Escherichia coli. Crude glycerol supplemented at 1–5 % (v/v) supported production of poly(3-hydroxybutyrate) (P(3HB)) in E. coli-ABCAh, which harbors the PHA synthetic genes for β-ketothiolase (PhaARe), acetoacetyl-CoA reductase (PhaBRe) of Ralstonia eutropha and Polyhydroxyalkanoate (PHA) synthase (PhaCAh) of Aeromonas hydrophila. The highest P(3HB) content and productivity of 14 wt% of cell dry weight and 0.6 g/L, respectively, were obtained at 1 % (v/v) glycerol concentration. Production of P(3HB-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)) was achieved using E. coli-ABCAhJAh, harboring genes for PhaARe, PhaBRe, PhaCAh, and the (R)-specific enoyl-CoA hydratase (PhaJAh) of A. hydrophila. This led to the copolymer content of 3 wt% of cell dry weight with 1 mol% of 3HHx. Molecular weight and degradation temperature of the polymers were in the range of 110–130 kDa and 295–299 °C, respectively. These results indicated that crude glycerol could be an attractive carbon source for economical production of PHAs with properties for industrial application.