Publication: Production of Polyhydroxyalkanoates from Crude Glycerol Using Recombinant Escherichia coli
Issued Date
2015-01-01
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ISSN
15662543
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2-s2.0-84924222152
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Polymers and the Environment. Vol.23, No.1 (2015), 38-44
Suggested Citation
Chitwadee Phithakrotchanakoon, Verawat Champreda, Sei ichi Aiba, Kusol Pootanakit, Sutipa Tanapongpipat Production of Polyhydroxyalkanoates from Crude Glycerol Using Recombinant Escherichia coli. Journal of Polymers and the Environment. Vol.23, No.1 (2015), 38-44. doi:10.1007/s10924-014-0681-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36016
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Title
Production of Polyhydroxyalkanoates from Crude Glycerol Using Recombinant Escherichia coli
Abstract
© 2014, Springer Science+Business Media New York. The high production cost of bio-based plastic polyhydroxyalkanoates (PHAs) limits their use as commercial products. Thus, systems for PHAs production from waste substrates could reduce production costs. Crude glycerol is a by-product of biodiesel fuel production and thus represents an inexpensive, abundant and promising carbon source for production of valorized fermentation products. In this study, industrial crude glycerol by-product from palm oil biodiesel production was used as the carbon source for production of PHAs by recombinant Escherichia coli. Crude glycerol supplemented at 1–5 % (v/v) supported production of poly(3-hydroxybutyrate) (P(3HB)) in E. coli-ABC<inf>Ah</inf>, which harbors the PHA synthetic genes for β-ketothiolase (PhaA<inf>Re</inf>), acetoacetyl-CoA reductase (PhaB<inf>Re</inf>) of Ralstonia eutropha and Polyhydroxyalkanoate (PHA) synthase (PhaC<inf>Ah</inf>) of Aeromonas hydrophila. The highest P(3HB) content and productivity of 14 wt% of cell dry weight and 0.6 g/L, respectively, were obtained at 1 % (v/v) glycerol concentration. Production of P(3HB-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)) was achieved using E. coli-ABC<inf>Ah</inf>J<inf>Ah</inf>, harboring genes for PhaA<inf>Re</inf>, PhaB<inf>Re</inf>, PhaC<inf>Ah</inf>, and the (R)-specific enoyl-CoA hydratase (PhaJ<inf>Ah</inf>) of A. hydrophila. This led to the copolymer content of 3 wt% of cell dry weight with 1 mol% of 3HHx. Molecular weight and degradation temperature of the polymers were in the range of 110–130 kDa and 295–299 °C, respectively. These results indicated that crude glycerol could be an attractive carbon source for economical production of PHAs with properties for industrial application.