Browsing by Author "Sutummaporn K."

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    Coding-Complete Genome Sequence of a Lumpy Skin Disease Virus Isolated during the 2021 Thailand Outbreak
    (2022-08-01) Paungpin W.; Sariya L.; Chaiwattanarungruengpaisan S.; Thongdee M.; Kornmatitsuk B.; Jitwongwai A.; Taksinoros S.; Sutummaporn K.; Boonmasawai S.; Nakthong C.; Mahidol University
    Lumpy skin disease (LSD) is an economically devastating and transboundary disease in cattle. Here, we report the coding-complete genome sequence of the LSDV72/PrachuapKhiriKhan/Thailand/2021 strain, which was isolated from an affected cow during the first LSD outbreak in Thailand in 2021. The sequence will be beneficial for future genomic studies of the virus.
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    Epidemiology of bluetongue virus infection among goat populations in central and western Thailand: Prevalence, risk factors, and serotype analysis
    (2025-03-01) Homat T.; Fujisawa Y.; Nakthong C.; Prompiram P.; Phonaknguen R.; Songkasupa T.; Satitvipawee P.; Chaichoun K.; Sutummaporn K.; Kornmatitsuk S.; Kornmatitsuk B.; Homat T.; Mahidol University
    The current status of bluetongue (BT) in Thailand remains unclear, and the serotypes of BT virus (BTV) have not been determined. This study aimed to 1) investigate the epidemiology of BTV infection among goat populations in central and western Thailand; 2) evaluate the test performance of conventional RT-PCR for BTV detection; and 3) identify the BTV serotypes presently circulating in the study area. The herd seroprevalence was 94.6 % (53/56, 95 % CI = 85.3–98.1), while the individual seroprevalence was 74.4 % (434/583, 95 % CI = 70.5–77.6). The odds of BTV infection were higher in goats in the western region (aOR = 10.16, 95 % CI = 5.02–20.55), with the presence of nearby ruminant farms (aOR = 2.21, 95 % CI = 1.23–4.00), and aged ≥3 years (aOR = 14.02, 95 % CI = 6.32–31.08) but lower in goats with an intensive rearing system (aOR = 0.18, 95 % CI = 0.09–0.39). For BTV detection, conventional RT-PCR presented 84.5 % sensitivity and 97.4 % specificity with an AUC of 0.910 (95 % CI = 0.852–0.967). Seven BTV serotypes (2, 3, 4, 7, 12, 16, and 21) were identified. Among these, BTV-2, 12, and 21 were the most prevalent, and BTV-4 was first detected in Southeast Asia. These results illustrated that BT is endemic and spreading widely among goat populations in the study area. Conventional RT-PCR developed in this study proved to be a reliable method for BTV detection. This research provides the first report of BTV serotype distribution in Thailand.
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    Molecular detection and characterization of lumpy skin disease viruses from outbreaks in Thailand in 2021
    (2022-09-01) Sariya L.; Paungpin W.; Chaiwattanarungruengpaisan S.; Thongdee M.; Nakthong C.; Jitwongwai A.; Taksinoros S.; Sutummaporn K.; Boonmasawai S.; Kornmatitsuk B.; Mahidol University
    Lumpy skin disease (LSD) is one of the most important transboundary and emerging diseases in cattle. The disease causes significant economic losses in animal production and trade worldwide. The first LSD outbreak was recorded in March 2021, at Roi-Et province in the northeastern region of Thailand. Thereafter, the disease had rapidly spread into neighbouring provinces and throughout the country. The aim of the present study was to provide information regarding to the molecular detection and characterization of LSD viruses from outbreaks in Thailand in 2021. There were 1,748,112 susceptible and 604,404 affected animals (n = 588,512 [36.30%], beef cattle; n = 12,367 [15.74%], dairy cattle and n = 3524 [7.35%], buffaloes). The morbidity and mortality rates were 34.57% and 3.47%, respectively, and the case fatality rate was 10.05% (60,713 deaths). Based on real-time polymerase chain reaction results, the p32 gene of LSD virus (LSDV) was detected more frequently in skin nodule samples (54/77, 70.13%) than in nasal swabs (26/55, 42.57%) and EDTA blood (16/77, 20.78%) samples. Moreover, the copy number of the p32 gene was higher in skin nodule samples than in nasal swab and EDTA blood samples (cycle threshold value = 21.94 ± 0.62 vs. 31.52 ± 0.66 and 34.27 ± 0.32, respectively). Furthermore, 29 (53.70%) of 54 capripoxvirus-positive skin nodule samples were successfully isolated from Madin–Darby bovine kidney cells, and the cytopathic effect was observed 72 h after inoculation. Based on the phylogenetic trees of the GPCR, ANK and RPO30 gene sequences, the LSDV isolates from Thailand were distinct from both the LSDV-field and LSDV-vaccine groups and were closely correlated with the LSDV strains isolated from mainland China, Hong Kong territory and Vietnam in 2020. Additionally, they could be a potential virulent vaccine-recombinant LSDV strain.
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    Pharmacological Activities of Fingerroot Extract and Its Phytoconstituents Against SARS-CoV-2 Infection in Golden Syrian Hamsters
    (2023-01-01) Kongratanapasert T.; Kongsomros S.; Arya N.; Sutummaporn K.; Wiriyarat W.; Akkhawattanangkul Y.; Boonyarattanasoonthorn T.; Asavapanumas N.; Kanjanasirirat P.; Suksatu A.; Sa-Ngiamsuntorn K.; Borwornpinyo S.; Vivithanaporn P.; Chutipongtanate S.; Hongeng S.; Ongphiphadhanakul B.; Thitithanyanont A.; Khemawoot P.; Sritara P.; Mahidol University
    Background: The outbreak of COVID-19 has led to the suffering of people around the world, with an inaccessibility of specific and effective medication. Fingerroot extract, which showed in vitro anti-SARS-CoV-2 activity, could alleviate the deficiency of antivirals and reduce the burden of health systems. Aim of Study: In this study, we conducted an experiment in SARS-CoV-2-infected hamsters to determine the efficacy of fingerroot extract in vivo. Materials and Methods: The infected hamsters were orally administered with vehicle control, fingerroot extract 300 or 1000 mg/kg, or favipiravir 1000 mg/kg at 48 h post-infection for 7 consecutive days. The hamsters (n = 12 each group) were sacrificed at day 2, 4 and 8 post-infection to collect the plasma and lung tissues for analyses of viral output, lung histology and lung concentration of panduratin A. Results: All animals in treatment groups reported no death, while one hamster in the control group died on day 3 post-infection. All treatments significantly reduced lung pathophysiology and inflammatory mediators, PGE2 and IL-6, compared to the control group. High levels of panduratin A were found in both the plasma and lung of infected animals. Conclusion: Fingerroot extract was shown to be a potential of reducing lung inflammation and cytokines in hamsters. Further studies of the full pharmacokinetics and toxicity are required before entering into clinical development.