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    Genetic characterization of Helicobacter pylori vacA and cagA genes in Thai gastro-duodenal and hepatobiliary patients
    (2017) Wongwarut Boonyanugomol; Narong Khuntikeo; Ake Pugkhem; Kookwan Sawadpanich; Chariya Hahnvajanawong; Phattharaphon Wongphutorn; Bandit Khampoosa; Chariya Chomvarin; Mahidol University. Amnatchareon Campus; Khon Kaen University. Faculty of Medicine. Department of Microbiology; Khon Kaen University. Faculty of Medicine. Department of Surgery; Khon Kaen University. Faculty of Medicine. Department of Medicine; Khon Kaen University. Faculty of Medicine. Liver Fluke and CholangiocarcinomaResearch Center,; King Mongkut’s University of Technology Thonburi. School of Bioinformatics and Systems Biology
    Introduction: H. pylorihas been detected in patients with hepatobiliary diseases. It is currently unclear whether the H. pyloridetected in hepatobiliary patients are genetically similar to those in gastro-duodenal patients. The aim of this study was to determine H. pylori vacAand cagA genotypes in Thai patients with gastro-duodenal and hepatobiliary diseases.Methodology: H. pyloriDNA was extracted from samples from gastric biopsies of gastro-duodenal patients (n=100) and from bile samples of hepatobiliary patients (n=80). The vacA and cagA genotypes were performed via polymerase chain reaction (PCR) followed by DNA sequencing.Results: The vacA m1 was found in Thai hepatobiliary patients (90%) at a higher rate compared with gastro-duodenal patients (50%).The combined vacA s1a+c/m1 were mostly found in Thai gastro-duodenal and hepatobiliary patients. The cagA gene was detected in 94% of patients with gastro-duodenal diseases compared with 28.8% in those with hepatobiliary diseases (p<0.05). On the other hand, the Western type cagA was more prominent among hepatobiliary patients (100%) than gastro-duodenal patients (57.4%), and this type was grouped into same cluster with Thai gastro-duodenal patients via phylogenetic analysis.Conclusions: Based on vacA and cagA analysis, we conclude that infection with H. pyloriin gastro-duodenal and hepatobiliary patients may be caused by the different H. pyloristrains.