- PublicationDystrobrevin is required postsynaptically for homeostatic potentiation at the Drosophila NMJ(2019-06-01) Salinee Jantrapirom; Wutigri Nimlamool; Piya Temviriyanukul; Somaieh Ahmadian; Cody J. Locke; Graeme W. Davis; Masamitsu Yamaguchi; Jasprina N. Noordermeer; Lee G. Fradkin; Saranyapin Potikanond; Kyoto Institute of Technology; University of California, San Francisco; Leiden University Medical Center - LUMC; Mahidol University; University of Massachusetts Medical School; Chiang Mai University© 2019 Elsevier B.V. Evolutionarily conserved homeostatic systems have been shown to modulate synaptic efficiency at the neuromuscular junctions of organisms. While advances have been made in identifying molecules that function presynaptically during homeostasis, limited information is currently available on how postsynaptic alterations affect presynaptic function. We previously identified a role for postsynaptic Dystrophin in the maintenance of evoked neurotransmitter release. We herein demonstrated that Dystrobrevin, a member of the Dystrophin Glycoprotein Complex, was delocalized from the postsynaptic region in the absence of Dystrophin. A newly-generated Dystrobrevin mutant showed elevated evoked neurotransmitter release, increased bouton numbers, and a readily releasable pool of synaptic vesicles without changes in the function or numbers of postsynaptic glutamate receptors. In addition, we provide evidence to show that the highly conserved Cdc42 Rho GTPase plays a key role in the postsynaptic Dystrophin/Dystrobrevin pathway for synaptic homeostasis. The present results give novel insights into the synaptic deficits underlying Duchenne Muscular Dystrophy affected by a dysfunctional Dystrophin Glycoprotein complex.
- PublicationEarly reduction of serum interleukin-6 levels as a predictor of clinical remission in systemic juvenile idiopathic arthritis(2019-06-01) Butsabong Lerkvaleekul; Soamarat Vilaiyuk; Faculty of Medicine, Ramathibodi Hospital, Mahidol University© 2019, Allergy and Immunology Society of Thailand. All rights reserved. Background: Interleukin (IL)-6 is the main proinflammatory cytokine in systemic juvenile idiopathic arthritis (SJIA). Objective: To determine if serial changes in serum IL-6 levels can predict outcomes of SJIA patients. Methods: This was a retrospective cohort study. Medical records of patients aged 2–19 years with active SJIA between January 2012 and February 2014 were reviewed. Baseline characteristics were recorded at enrollment. Serum IL-6 levels were measured at enrollment and at 2–4 weeks, 6–8 weeks, 3 months, and 6 months thereafter. Treatment response and clinical remission were assessed after 2 years of follow-up. Results: Of the 35 patients with active SJIA, 16 were in remission at the end of the study. IL-6 levels in the remission group returned to normal within 6 months, whereas they remained persistently high in the non-remission group. At the 3-month follow-up, patients were assigned to groups A and B based on reductions in serum IL-6 levels of > 50% and ≤ 50%, respectively. At the end of the study, more patients in group A (72.2%) than in group B (17.6%) achieved clinical remission (p < 0.05). After multivariate analysis, a > 50% reduction in serum IL-6 levels at the 3-month follow-up visit was a predictor of clinical remission at 2 years (odds ratio 22.74, 95% confidence intervals 2.16–239.85, p < 0.01). Conclusions: An early reduction in serum IL-6 levels is significantly associated with clinical remission at 2 years in SJIA patients. Monitoring of serial changes in serum IL-6 levels is beneficial for predicting clinical remission.
- PublicationBiogas production from single digestion of napier grass hydrolysate and co-digestion of solid fraction of microwave acid pretreated napier grass with swine manure(2019-07-01) Prawit Kongjan; Alissara Reungsang; Naphatsarnan Phasukarratchai; Sureewan Sittijunda; Faculty of Environment and Resource Studies, Mahidol University; Khon Kaen University; Prince of Songkla University© All right Reserved 2019 The biogas production from hydrolysate and solid fraction of microwave acid pretreated Napier grass was investigate in the batch experiments. Factors influencing methane production including initial pH, inoculum concentration, and carbon to nitrogen ratio (C/N ratio) were investigated. For the hydrolysate of Napier grass, maximum methane production (MP) and methane production rate (MPR) of 621.31 mL-CH4/L and 0.72 mLCH4/L h were obtained at initial pH of 8 and inoculum concentration of 15 g-VSS/L. Using the solid fraction of pretreated Napier grass co-digested with swine manure, maximum MP and MPR of 630.05 mL-CH4/L and 0.74 mLCH4/L h were obtained at C/N ratio of 21.03. Main methanogenic bacteria found in the hydrolysate and solid fraction fermentation of pretreated Napier grass co-digested with swine manure were Methanosarcina sp. Methanoregula sp. Methanospirillum sp. Methanocullues sp. and Methanothrix sp. Overall energy production from Napier grass hydrolysate and the solid fraction of pretreated Napier grass co-digested with swine manure was 7.99 kJ/g-VSadded.
- PublicationVascularized Parenchymal Mass Preserved with Partial Nephrectomy: Functional Impact and Predictive Factors(2019-02-01) Jitao Wu; Chalairat Suk-Ouichai; Wen Dong; Zhiling Zhang; Hajime Tanaka; Yanbo Wang; Elvis Caraballo; Erick M. Remer; Jianbo Li; Sudhir Isharwal; Robert Abouassaly; Steven C. Campbell; The First Bethune Hospital of Jilin University; Yantai Yuhuangding Hospital; Sun Yat-Sen University Cancer Center; Cleveland Clinic Foundation; Faculty of Medicine, Siriraj Hospital, Mahidol University; Tokyo Medical and Dental University; Sun Yat-sen University© 2018 European Association of Urology Background: Percentage parenchymal mass preserved (PPMP) is a key determinant of functional outcomes after partial nephrectomy (PN); however, predictors of PPMP have not been defined. Objective: To provide a comprehensive analysis of the functional impact of and potential predictive factors for PPMP. Design, setting, and participants: We analyzed data for 464 patients managed with PN at our center with necessary studies to determine vascularized parenchymal mass and function preserved within the operated kidney. PPMP was measured from computed tomography scans <2 mo before and 3–12 mo after PN. Intervention: PN. Outcome measurements/statistical analysis: Recovery from ischemia was defined as percentage ipsilateral glomerular filtration rate (GFR) preserved normalized by PPMP. We used Pearson correlation to evaluate the relationships between GFR preserved and PPMP. Multivariable logistic regression was used to assess predictors of PPMP. Result and limitations: Ninety-six patients (21%) had a solitary kidney. The median tumor size and RENAL score were 3.5 cm and 8, respectively. Cold/warm ischemia were utilized in 183/281 patients for which the median ischemia time were 28/20 min. The median preoperative and postoperative vascularized parenchymal mass in the operated kidney were 194 and 157 cm3, respectively, resulting in median PPMP of 84%. GFR preservation correlated strongly with PPMP (r = 0.64; p < 0.001). Recovery from ischemia was suboptimal (<80%) in 71 patients (15%), while suboptimal PPMP (<80%) was a more common adverse event, occurring in 160 patients (34%; p < 0.001). Multivariable analysis demonstrated that greater tumor size and complexity were associated with lower PPMP (p ≤ 0.04), while solitary kidney and hypothermia were associated with higher PPMP (p < 0.001). Longer ischemia time was also associated with lower PPMP (p = 0.003), probably reflecting the complexity of the surgery. Limitations include the retrospective design. Conclusion: PPMP correlates strongly with functional outcomes after PN, and lower PPMP is the most common and important source of functional decline after PN. Larger tumors, greater tumor complexity, and prolonged ischemia time were associated with lower PPMP, while PPMP tended to be greater for solitary kidneys, confirming that PPMP is a modifiable factor. Patient summary: Kidney function after partial nephrectomy primarily depends on the amount of vascularized kidney preserved by the procedure. Lower recovery of function is seen when operating on larger tumors in unfavorable locations, but preservation of the parenchymal mass can be improved when truly necessary, such as when operating on a tumor in a solitary kidney. Suboptimal percentage parenchymal mass preservation (PPMP) is the predominant cause of lower functional recovery after partial nephrectomy. Larger tumors and greater tumor complexity are associated with lower PPMP, while PPMP was greater for tumors in a solitary kidney, suggesting that PPMP is a modifiable parameter.
- PublicationGeneration of two induced pluripotent stem cell lines (MUSIi011-A and MUSIi011-B) from peripheral blood T lymphocytes of a healthy individual(2019-08-01) Ratchapong Netsrithong; Nutchanawan Promnakhon; Bootsakorn Boonkaew; Chinnavuth Vatanashevanopakorn; Kovit Pattanapanyasat; Methichit Wattanapanitch; Faculty of Medicine, Siriraj Hospital, Mahidol University© 2019 Activated T lymphocytes of a healthy individual were reprogrammed to induced pluripotent stem cells (iPSCs) using Sendai viral vectors. Two iPSC lines, MUSIi011-A and MUSIi011-B, were established and characterized for the expression of pluripotent markers. Both iPSC lines were able to differentiate into cells of three embryonic germ layers via embryoid body formation, exhibited normal karyotypes and were free of viral genome and transgenes at passage 15. These T lymphocyte-derived iPSCs (T-iPSCs) represent a useful starting cell source for developing next-generation immune cells such as chimeric antigen receptor (CAR)-engineered iPSC-derived T lymphocytes for the application in adoptive immunotherapy.
- PublicationThe management of reputational risks in banks: Findings from Germany and Switzerland(2019-07-01) Simon Zaby; Michael Pohl; Mahidol University; Steinbeis-Hochschule-Berlin© The Author(s) 2019. This article identifies reputation-risk-relevant factors for banks, and the focus will be placed on the development of an indicator-based model for the assessment of reputation. Requirements and insights are based on a survey of credit institutions in Germany and Switzerland, which have been predominantly affected during the financial crisis by aptly nascent risks and which are thereby also partially affected even today. Reputation level can be considered as a temporally dynamical phenomenon which predominantly develops depending on the changes in the reputation drivers and expectations of the groups of stakeholders. This control parameter can be determined with the aid of Reputation Index Points (RIP). Efficient reputation risk management can, in the future, help prevent negative spillover effects from banks which face difficulties from the society or the taxpayers.
- PublicationSelf-Flickering Visual Stimulus based on Visual illusion for SSVEP-based BCI System(2019-01-10) Yunyong Punsawad; Nannaphat Siribunyaphat; Yodchanan Wongsawat; Silpakorn University; Mahidol University© 2018 IEEE. This paper proposes the use of the windmill pattern visual stimulus to induce human vision by employing a phenomenon of visual illusion for steady-state visual evoked potential (SSVEP)-based brain-computer interface (BCI) system. We had to explore the brain response to the flickering pattern as windmill pattern, three BCI commands can be generated by using three different windmill patterns. SSVEP technique was used to detect the response. The average accuracy of classification was approximately 80.5%. With the proposed visual stimulus pattern, it can reduce eye fatigue and increase the number of commands for the existing SSVEP-based BCI. Therefore, the proposed visual stimulus pattern can be used for practical BCI applications.
- PublicationDual Analysis of Loss to Follow-up for Perinatally HIV-Infected Adolescents Receiving Combination Antiretroviral Therapy in Asia(2019-12-15) Adam W. Bartlett; Pagakrong Lumbiganon; Thahira A. Jamal Mohamed; Keswadee Lapphra; Dina Muktiarti; Quy Tuan Du; Rawiwan Hansudewechakul; Penh Sun Ly; Khanh Huu Truong; Lam Van Nguyen; Thanyawee Puthanakit; Tavitiya Sudjaritruk; Kulkanya Chokephaibulkit; Viet Chau Do; Nagalingeswaran Kumarasamy; Nik Khairulddin Nik Yusoff; Nia Kurniati; Moy Siew Fong; Dewi Kumara Wati; Revathy Nallusamy; Annette H. Sohn; Azar Kariminia; VHS Medical Centre India; National Hospital of Pediatrics Hanoi; Universitas Udayana; University of Indonesia, RSUPN Dr. Cipto Mangunkusumo; Chulalongkorn University; Kirby Institute; Faculty of Medicine, Khon Kaen University; Kuala Lumpur Hospital; The HIV Netherlands Australia Thailand Research Collaboration; Faculty of Medicine, Siriraj Hospital, Mahidol University; Chiang Mai University; Children's Hospital 2; National Center for HIV/AIDS; Children's Hospital 1; Hospital Raja Perempuan Zainab II; Foundation for AIDS Research; Chiangrai Prachanukroh Hospital; Hospital Likas; Penang HospitalBACKGROUND: Perinatally HIV-infected adolescents (PHIVA) are an expanding population vulnerable to loss to follow-up (LTFU). Understanding the epidemiology and factors for LTFU is complicated by varying LTFU definitions. SETTING: Asian regional cohort incorporating 16 pediatric HIV services across 6 countries. METHODS: Data from PHIVA (aged 10-19 years) who received combination antiretroviral therapy 2007-2016 were used to analyze LTFU through (1) an International epidemiology Databases to Evaluate AIDS (IeDEA) method that determined LTFU as >90 days late for an estimated next scheduled appointment without returning to care and (2) the absence of patient-level data for >365 days before the last data transfer from clinic sites. Descriptive analyses and competing-risk survival and regression analyses were used to evaluate LTFU epidemiology and associated factors when analyzed using each method. RESULTS: Of 3509 included PHIVA, 275 (7.8%) met IeDEA and 149 (4.3%) met 365-day absence LTFU criteria. Cumulative incidence of LTFU was 19.9% and 11.8% using IeDEA and 365-day absence criteria, respectively. Risk factors for LTFU across both criteria included the following: age at combination antiretroviral therapy initiation <5 years compared with age ≥5 years, rural clinic settings compared with urban clinic settings, and high viral loads compared with undetectable viral loads. Age 10-14 years compared with age 15-19 years was another risk factor identified using 365-day absence criteria but not IeDEA LTFU criteria. CONCLUSIONS: Between 12% and 20% of PHIVA were determined LTFU with treatment fatigue and rural treatment settings consistent risk factors. Better tracking of adolescents is required to provide a definitive understanding of LTFU and optimize evidence-based models of care.
- PublicationAnalysis of the Zika and Japanese Encephalitis Virus NS5 Interactomes(2019-08-02) Duangnapa Kovanich; Chonticha Saisawang; Potchaman Sittipaisankul; Suwipa Ramphan; Nuttiya Kalpongnukul; Poorichaya Somparn; Trairak Pisitkun; Duncan R. Smith; Chulalongkorn University; Mahidol University© 2019 American Chemical Society. Mosquito-borne flaviviruses, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), are major human pathogens. Among the flaviviral proteins, the nonstructural protein 5 (NS5) is the largest, most conserved, and major enzymatic component of the viral replication complex. Disruption of the common key NS5-host protein-protein interactions critical for viral replication could aid in the development of broad-spectrum antiflaviviral therapeutics. Hundreds of NS5 interactors have been identified, but these are mostly DENV-NS5 interactors. To this end, we sought to investigate the JEV- and ZIKV-NS5 interactomes using EGFP immunoprecipitation with label-free quantitative mass spectrometry analysis. We report here a total of 137 NS5 interactors with a significant enrichment of spliceosomal and spliceosomal-associated proteins. The transcription complex Paf1C and phosphatase 6 were identified as common NS5-associated complexes. PAF1 was shown to play opposite roles in JEV and ZIKV infections. Additionally, we validated several NS5 targets and proposed their possible roles in infection. These include lipid-shuttling proteins OSBPL9 and OSBPL11, component of RNAP3 transcription factor TFIIIC, minichromosome maintenance, and cochaperone PAQosome. Mining this data set, our study expands the current interaction landscape of NS5 and uncovers several NS5 targets that are new to flavivirus biology.
- PublicationAssessing quality of life for multidrug-resistant and extensively drug-resistant tuberculosis patients(2019-06-01) Nam Xuan Vo; Thy Bui Xuan Doan; Di Ngoc Kha Vo; Tu Kim Tran; Trung Quang Vo; Ton-Duc-Thang University; Mahidol University; Pham Ngoc Thach University of MedicineObjective: One can hypothesize that Mycobacterium genus originated more than 150 million years ago and has evolved to become one of the leading lethal infectious diseases. Multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) patients are directly affected by the disease and other subjective issues, such as related diseases, medical costs and social issues, which all have negative impacts on patient quality of life (QOL). Our purpose is to define the status of health-related QOL for international MDR-TB and XDR-TB patients. Methods: Systematic review is a good method for searching and selecting related researches and articles. As such, we have searched for and cited related articles on reputable databases, such as PubMed, Cochrance, and Google Scholar. A data overview was performed to draw conclusions and results on the QOL of MDR-TB and XDR-TB patients. Results: A total of 18 articles were included, using instruments from the World Health Organization, Euroqol, Short Form, AQ and the Seattle Obstructive Lung Disease Questionnaire. The QOL of MDR-TB and XDR-TB patients was found to be compromised due to the strong resistance of Mycobacterium tuberculosis, economic pressure and community alienation. Conclusions: A number of QOL and health-related QOL studies on MDR-TB and XDR-TB patients are limited, especially with XDR-TB patients. Significant numbers of MDR-TB and XDR-TB patients still have sequelae after completing treatment, reducing the health-related QOL among these patients.