Scopus 2019
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Publication Metadata only Structural and Functional Studies of the Membrane-Binding Domain of NADPH-Cytochrome P450 Oxidoreductase(2019-05-14) Chuanwu Xia; Anna L. Shen; Panida Duangkaew; Rattanawadee Kotewong; Pornpimol Rongnoparut; Jimmy Feix; Jung Ja P. Kim; McArdle Laboratory for Cancer Research; Silpakorn University; Mahidol University; Medical College of Wisconsin© 2019 American Chemical Society. NADPH-cytochrome P450 oxidoreductase (CYPOR), the essential flavoprotein of the microsomal cytochrome P450 monooxygenase system, is anchored in the phospholipid bilayer by its amino-terminal membrane-binding domain (MBD), which is necessary for efficient electron transfer to cytochrome P450. Although crystallographic and kinetic studies have established the structure of the soluble catalytic domain and the role of conformational motions in the control of electron transfer, the role of the MBD is largely unknown. We examined the role of the MBD in P450 catalysis through studies of amino-terminal deletion mutants and site-directed spin labeling. We show that the MBD spans the membrane and present a model for the orientation of CYPOR on the membrane capable of forming a complex with cytochrome P450. EPR power saturation measurements of CYPOR mutants in liposomes containing a lipid/Ni(II) chelate identified a region of the soluble domain interacting with the membrane. The deletion of more than 29 residues from the N-terminus of CYPOR decreases cytochrome P450 activity concomitant with alterations in electrophoretic mobility and an increased resistance to protease digestion. The altered kinetic properties of these mutants are consistent with electron transfer through random collisions rather than via formation of a stable CYPOR-P450 complex. Purified MBD binds weakly to cytochrome P450, suggesting that other interactions are also required for CYPOR-P450 complex formation. We propose that the MBD and flexible tether region of CYPOR, residues 51-63, play an important role in facilitating the movement of the soluble domain relative to the membrane and in promoting multiple orientations that permit specific interactions of CYPOR with its varied partners.Publication Metadata only Predictors of NOAC versus VKA use for stroke prevention in patients with newly diagnosed atrial fibrillation: Results from GARFIELD-AF(2019-07-01) Sylvia Haas; A. John Camm; Jean Pierre Bassand; Pantep Angchaisuksiri; Frank Cools; Ramon Corbalan; Harry Gibbs; Barry Jacobson; Yukihiro Koretsune; Lorenzo G. Mantovani; Frank Misselwitz; Elizaveta Panchenko; Hany Ibrahim Ragy; Janina Stepinska; Alexander GG Turpie; Jitendra PS Sawhney; Jan Steffel; Toon Wei Lim; Karen S. Pieper; Saverio Virdone; Freek WA Verheugt; Ajay K. Kakkar; National University Health System; Instytut Kardiologii im. Prymasa Tysiaclecia Stefana Kardynała Wyszynskiego; National Medical Research Center of Cardiology, Moscow; Osaka National Hospital; McMaster University; Universite de Franche-Comte; Duke Clinical Research Institute; Pontificia Universidad Católica de Chile; St George's University of London; Algemeen Ziekenhuis Klina; Sir Ganga Ram Hospital; Our Lady Hospital - Amsterdam; UCL; Technical University of Munich; UniversitatsSpital Zurich; University of Witwatersrand; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Thrombosis Research Institute; University of Milano - Bicocca; Alfred Hospital; Bayer AG; National Heart Institute© 2019 The Authors Introduction: A principal aim of the Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) was to document changes in treatment practice for patients with newly diagnosed atrial fibrillation during an era when non–vitamin K antagonist oral anticoagulants (NOACs) were becoming more widely adopted. In these analyses, the key factors which determined the choice between NOACs and vitamin K antagonists (VKAs) are explored. Methods: Logistic least absolute shrinkage and selection operator regression determined predictors of NOAC and VKA use. Data were collected from 24,137 patients who were initiated on AC ± antiplatelet (AP) therapy (NOAC [51.4%] or VKA [48.6%]) between April 2013 and August 2016. Results: The most significant predictors of AC therapy were country, enrolment year, care setting at diagnosis, AF type, concomitant AP, and kidney disease. Patients enrolled in emergency care or in the outpatient setting were more likely to receive a NOAC than those enrolled in hospital (OR 1.16 [95% CI: 1.04-1.30], OR: 1.15 [95% CI: 1.05-1.25], respectively). NOAC prescribing seemed to be favored in lower-risk groups, namely, patients with paroxysmal AF, normotensive patients, and those with moderate alcohol consumption, but also the elderly and patients with acute coronary syndrome. By contrast, VKAs were preferentially used in patients with permanent AF, moderate to severe kidney disease, heart failure, vascular disease, and diabetes and with concomitant AP. Conclusion: GARFIELD-AF data highlight marked heterogeneity in stroke prevention strategies globally. Physicians are adopting an individualized approach to stroke prevention where NOACs are favored in patients with a lower stroke risk but also in the elderly and patients with acute coronary syndrome.Publication Metadata only Increased serum glypican-3 is associated with liver stiffness and hepatic dysfunction in children with biliary atresia(2019-01-01) Kanjaporn Sirisomboonlarp; Wanvisa Udomsinprasert; Ellie McConachie; Thamonwan Woraruthai; Yong Poovorawan; Sittisak Honsawek; Chulalongkorn University; Mahidol University© 2019 Termedia Publishing House Ltd. All rights reserved. Aim of the study: Biliary atresia (BA) is an uncommon disorder of the liver and bile ducts affecting infants and is characterized by progressive fibrosclerosing obstruction of the extrahepatic biliary tree leading to end-stage liver failure. The purpose of this study was to determine serum glypican-3 (GPC3) levels and liver stiffness in children with BA and the correlation of glypican-3 with clinical parameters. Material and methods: Seventy-five post-Kasai BA patients and 28 healthy age-matched controls were registered. Serum GPC3 levels were examined by enzyme-linked immunosorbent assay. Liver stiffness measurement was analyzed by transient elastography. Results: BA patients had significantly greater serum GPC3 and liver stiffness values than controls (p < 0.001). Serum GPC3 and liver stiffness values were significantly higher in jaundiced BA patients than in non-jaundiced BA patients (p < 0.001). Additionally, serum glypican-3 was associated with liver stiffness and serum total bilirubin (p < 0.001, respectively). Conclusions: Elevated serum GPC3 levels were associated with hepatic dysfunction and the severity of BA. As a result, serum GPC3 and liver stiffness might serve as biomarkers reflecting the deterioration of hepatic function and the outcome in post-Kasai BA.Publication Metadata only Starvation promotes autophagy-associated maturation of the testis in the giant freshwater prawn, macrobrachium rosenbergii(2019-09-01) Wilairat Kankuan; Chaitip Wanichanon; Federica Morani; Sirorat Thongrod; Rossella Titone; Tanapan Siangcham; Matilde Masini; Michela Novelli; Prasert Sobhon; Ciro Isidoro; Università di Pisa; Mahidol University; Università degli Studi del Piemonte Orientale "Amedeo Avogadro"© 2019 Kankuan, Wanichanon, Morani, Thongrod, Titone, Siangcham, Masini, Novelli, Sobhon and Isidoro. Autophagy is a degradative process of cellular components accomplished through an autophagosomal-lysosomal pathway. It is an evolutionary conserved mechanism present in all eukaryotic cells, and it plays a fundamental role in maintaining tissue homeostasis both in vertebrates and invertebrates. Autophagy accompanies tissue remodeling during organ differentiation. Several autophagy-related genes and proteins show significant upregulations following nutrient shortage (i.e., starvation). In our previous study, we found that in female giant freshwater prawns subjected to a short period of starvation autophagy was up-regulated in consonant with ovarian maturation and oocyte differentiation. Whether and how starvation-induced autophagy impacts on testicular maturation and spermatogenesis of the male prawns remained to be investigated. In this study, we analyzed the effects of starvation on histological and cellular changes in the testis of the giant freshwater prawn Macrobrachium rosenbergii that paralleled the induction of autophagy. Under short starvation condition, the male prawns showed increased gonado-somatic index, increased size, and late stage of maturation of seminiferous tubules, which contained increased number of spermatozoa. Concurrently, the number of autophagy vacuoles and autophagy flux, as monitored by transmission electron microscopy and the autophagic marker LC3, increased in the testicular cells, indicating that a short period of starvation could induce testicular maturation and spermatogenesis in male M. rosenbergii along with modulation of autophagy.Publication Metadata only Forest certification: More than a market-based tool, experiences from the Asia Pacific region(2019-05-01) Allison Lewin; Karen Mo; Henry Scheyvens; Sara Gabai; World Wildlife Fund; Nature Conservancy; Mahidol University; Institute for Global Environmental Strategies© 2019 by the authors. Over the last 25 years, the global area of certified forests has grown rapidly and voluntary forest certification has become recognized as an effective tool to engage international markets in improving sustainability within forest management units. However, the bulk of this growth has occurred in North America, Northern Europe, Australia, and New Zealand, with relatively limited uptake in the tropics. Since its creation, forest certification has been largely understood as a "market-based" mechanism, in contrast to government-led policies and regulations. Through the experience of the Responsible Asia Forestry and Trade (RAFT) partnership in the Asia Pacific region, we find that the framing of forest certification as voluntary and market-based, and as a mechanism to overcome governance failure, has created an artificial dichotomy. In this dichotomy, voluntary certification and regulatory measures to promote sustainable forest management are conceived of and pursued largely independently. We argue that it is more constructive to view them as complementary approaches that share a common goal of increasing sustainability across the forestry sector. In practice, forest certification interacts with conventional governance institutions and mechanisms. Understanding these interactions and their implications, as well as additional possibilities for interaction, will help in realizing the full potential of forest certification.Publication Metadata only Reliability and validity of the Thai Drug Hypersensitivity Quality of Life Questionnaire: a multi-center study(2019-08-01) Yuda Chongpison; Pawinee Rerknimitr; Cameron Hurst; Pungjai Mongkolpathumrat; Sirinoot Palapinyo; Leena Chularojanamontri; Yuttana Srinoulprasert; Ticha Rerkpattanapipat; Kumutnart Chanprapaph; Wareeporn Disphanurat; Panlop Chakkavittumrong; Napatra Tovanabutra; Chutika Srisuttiyakorn; Chonlaphat Sukasem; Papapit Tuchinda; Ilaria Baiardini; Jettanong Klaewsongkram; Humanitas University; Chulalongkorn University; King Chulalongkorn Memorial Hospital, Faculty of Medicine Chulalongkorn University; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Faculty of Medicine, Thammasat University; Faculty of Medicine, Siriraj Hospital, Mahidol University; Phramongkutklao College of Medicine; Chiang Mai University© 2018 The Author(s) 2018. Published by Oxford University Press in association with the International Society for Quality in Health Care. All rights reserved. Objective: To adapted the Drug Hypersensitivity Quality of Life (DrHy-Q) Questionnaire from Italian into Thai and assessed its validity and reliability. Design: Prospectively recruited during January 2012-May 2017. Setting: Multicenter; six Thai tertiary university hospitals. Study Participants: Total of 306 patients with physician-diagnosed drug hypersensitivity. Interventions: Internal consistency and test-retest reliability were evaluated among 68 participants using Cronbach's ? and intra-class correlation coefficient (ICC). The validity of Thai DrHy-Q was assessed among 306 participants who completed World Health Organization Quality of Life-BREF (WHOQOL-BREF-THAI). Construct and divergent validities were assessed for Thai DrHy-Q. Known-groups validity assessing discriminating ability was conducted in Thai DrHy-Q and WHOQOL-BREF-THAI. Main outcome measures: Validity; reliability; single vs. multiple drug allergy; non-severe cutaneous adverse reactions (SCAR) vs. SCAR. Results: Thai DrHy-Q showed good reliability (Cronbach's ? = 0.94 and ICC = 0.8). Unidimensional factor structure was established by confirmatory factor analysis (CFI&TLI = 0.999, RMSEA = 0.02). Divergent validity was confirmed by weak correlation between Thai DrHy-Q and WHOQOL-BREF-THAI domains (Pearson's r = -0.41 to -0.19). Known-groups validity of Thai DrHy-Q was confirmed with significant difference between patients with and without life-threatening SCAR (P = 0.02) and patients with multiple implicated drug classes vs. those with one class (P < 0.01); while WHOQOL-BREF-THAI could differentiate presence of life-threatening SCAR (P < 0.01) but not multiple-drug allergy. Conclusions: Thai DrHy-Q was reliable and valid in evaluating quality of life among patients with drug hypersensitivity. Thai DrHy-Q was able to discriminate serious drug allergy phenotypes from non-serious manifestations in clinical practice and capture more specific drug-hypersensitivity aspects than WHOQOL-BREF-THAI.Publication Metadata only Validity and reliability of the Thai version of Rome IV diagnostic questionnaires for pediatric gastrointestinal disorders(2019-01-01) S. Siajunboriboon; T. Ngoenmak; P. Tanpowpong; M. Sarawit; S. Treepongkaruna; Naresuan University; Faculty of Medicine, Ramathibodi Hospital, Mahidol University© Journal of the medical association of Thailand 2019. Objectives: To produce the Thai version of the Rome IV Diagnostic Questionnaires for Pediatric Gastrointestinal Disorders for children and adolescents, and assess their validity and reliability. Materials and Methods: The original English version of Rome IV Diagnostic Questionnaires for Pediatric Gastrointestinal Disorders, both parent-report and child self-report questionnaires, consisted of 5 sections. The questionnaires were forward translated into Thai. The Thai version was then back translated into English by a professional translator. The original and the back-translated versions were compared and validated using index of item-objective congruence (IOC). Then, reliability tests of the final Thai version were performed with 50 children and 50 parents and analyzed by Cronbach’s alpha coefficient. Retest of the questionnaires was evaluated in all subjects and analyzed by intra-class correlation coefficient (ICC). Results: The IOC of the Thai parent-report and self-report questionnaires were 0.87 and 0.91, respectively. Cronbach’s alpha coefficients of the parent-report and self-report questionnaires were 0.94 and 0.96, respectively. The ICC was 0.99 for the parent-report and self-report questionnaires. Conclusion: The Thai version of the Rome IV Diagnostic Questionnaires for Pediatric Gastrointestinal Disorders for children and adolescents, both parent-report and self-report, provide good validity and good reliability and can be used for research on functional gastrointestinal disorders in Thai children and adolescents.Publication Metadata only Diagnostic performance of electrocardiographic criteria for left ventricular hypertrophy among various body mass index groups compared to diagnosis by cardiac magnetic resonance imaging(2019-07-01) Veerawat Nomsawadi; Rungroj Krittayaphong; Faculty of Medicine, Siriraj Hospital, Mahidol University© 2019 Wiley Periodicals, Inc. Background: Presence of left ventricular hypertrophy (LVH) increases the risk for cardiovascular event. Many electrocardiographic (ECG) criteria can be used to diagnose LVH; however, high body mass index (BMI) may reduce ECG amplitudes.The aim of this study was to investigate the diagnostic performance of ECG criteria for diagnosing LVH among various BMI groups compared to diagnosis by cardiac magnetic resonance (CMR) imaging. Methods: Patients who were referred for CMR were enrolled. CMR and ECG were performed on the same day. Left ventricular function, volume, and mass were calculated from CMR. Standard ECG criteria were measured, including: Cornell voltage, Cornell product, Romhilt-Estes point score system, Sokolow-Lyon index, and Sokolow-Lyon-Rappaport index. Diagnostic performance of each ECG criterion was calculated and analyzed in the following four BMI groups: underweight (<18.5 kg/m2), normal (18.5–22.9 kg/m2), overweight (23–24.9 kg/m2), and obese (≥25 kg/m2). Results: Of the 1,882 patients that were included, 67 were underweight, 459 were normal weight, 434 were overweight, and 922 were obese. LVH was diagnosed in 34 (50.7%) underweight, 144 (31.4%) normal weight, 100 (23.0%) overweight, and 181 (19.6%) obese patients. Overall specificity of ECG was high (0.89–0.95), and overall sensitivity was low (0.25–0.37). The specificity of each ECG criterion was similar among BMI groups; however, the sensitivity of ECG criteria demonstrated a decreasing trend in the higher BMI groups. Conclusion: All ECG criteria demonstrated relatively high specificity and relatively low sensitivity. Although the specificity across groups remained similar, higher BMI was found to be associated with decreased sensitivity.Publication Metadata only Optimal dosing of dihydroartemisinin-piperaquine for seasonal malaria chemoprevention in young children(2019-12-01) Palang Chotsiri; Issaka Zongo; Paul Milligan; Yves Daniel Compaore; Anyirékun Fabrice Somé; Daniel Chandramohan; Warunee Hanpithakpong; François Nosten; Brian Greenwood; Philip J. Rosenthal; Nicholas J. White; Jean Bosco Ouédraogo; Joel Tarning; London School of Hygiene & Tropical Medicine; University of California, San Francisco; Mahidol University; Nuffield Department of Clinical Medicine; Institut de Recherche en Sciences de la Santé© 2019, The Author(s). Young children are the population most severely affected by Plasmodium falciparum malaria. Seasonal malaria chemoprevention (SMC) with amodiaquine and sulfadoxine-pyrimethamine provides substantial benefit to this vulnerable population, but resistance to the drugs will develop. Here, we evaluate the use of dihydroartemisinin-piperaquine as an alternative regimen in 179 children (aged 2.33–58.1 months). Allometrically scaled body weight on pharmacokinetic parameters of piperaquine result in lower drug exposures in small children after a standard mg per kg dosage. A covariate-free sigmoidal E MAX -model describes the interval to malaria re-infections satisfactorily. Population-based simulations suggest that small children would benefit from a higher dosage according to the WHO 2015 guideline. Increasing the dihydroartemisinin-piperaquine dosage and extending the dose schedule to four monthly doses result in a predicted relative reduction in malaria incidence of up to 58% during the high transmission season. The higher and extended dosing schedule to cover the high transmission period for SMC could improve the preventive efficacy substantially.Publication Metadata only Can standard deviation of overnight pulse oximetry be used to screen childhood obstructive sleep apnea(2019-04-01) Siriporn Warapongmanupong; Aroonwan Preutthipan; Faculty of Medicine, Ramathibodi Hospital, Mahidol University© 2019 Elsevier B.V. Objectives: Pulse oximetry (PO) has been frequently used as an alternative test to polysomnography (PSG) in children. We conducted this study to determine which statistical parameters obtained from overnight PO monitoring would be most suitable and to evaluate its diagnostic performance. Methods: We prospectively recruited children with snoring referred for PSG. Subjects were monitored with PO while performing PSG. Eight statistical parameters of SpO 2 data were analyzed to identify which had the best diagnostic performance as assessed by the area under the receiver-operating characteristic curve (AUC). To validate this parameter (which was found to be the standard deviation, SD) in a larger population, we retrospectively extracted raw data of SpO 2 from our previous PSG records, calculated the SD of each patient, and assessed its AUC. Results: A total of 166 children were recruited in the first phase. SD of SpO 2 was found to have the largest AUC. In the second phase, raw data of 457 patients were extracted. SD of SpO 2 correlated well with the apnea/hypopnea index (AHI) (r = 0.6, P < 0.001). For diagnosis of moderate to severe obstructive sleep apnea (OSA) (AHI ≥5 events/h), AUC was 0.74. SD of SpO 2 ≥1.06 representing mean + 2SD of normal to mild OSA (AHI <5) provided 97% specificity and 92% positive predictive value. The positive likelihood ratio was 11. Conclusion: Calculating the SD of SpO 2 , which quantifies the amount of dispersion of SpO 2 values, is a useful initial investigation in childhood OSA. An SD ≥ 1.06 can predict moderate to severe OSA with confidence. This parameter is simple, practical, and readily accessible.Publication Metadata only Molecular signaling mechanisms of renal gluconeogenesis in nondiabetic and diabetic conditions(2019-06-01) Myat Theingi Swe; Anchalee Pongchaidecha; Varanuj Chatsudthipong; Nipon Chattipakorn; Anusorn Lungkaphin; Mahidol University; Chiang Mai University; University of Medicine 1© 2018 Wiley Periodicals, Inc. The kidneys are as involved as the liver in gluconeogenesis which can significantly contribute to hyperglycemia in the diabetic condition. Substantial evidence has demonstrated the overexpression of rate-limiting gluconeogenic enzymes, especially phosphoenolpyruvate carboxykinase and glucose 6 phosphatase, and the accelerated glucose release both in the isolated proximal tubular cells and in the kidneys of diabetic animal models and diabetic patients. The aim of this review is to provide an insight into the mechanisms that accelerate renal gluconeogenesis in the diabetic conditions and the therapeutic approaches that could affect this process in the kidney. Increase in gluconeogenic substrates, reduced insulin concentration or insulin resistance, downregulation of insulin receptors and insulin signaling, oxidative stress, and inappropriate activation of the renin–angiotensin system are likely to participate in enhancing renal gluconeogenesis in the diabetic milieu. Several studies have suggested that controlling glucose metabolism at the renal level favors effective overall glycemic control in both type 1 and type 2 diabetes. Therefore, renal gluconeogenesis may be a promising target for effective glycemic control as a therapeutic strategy in diabetes.Publication Metadata only Using Cascade Generalization and Neural Networks to Select Cryotherapy method for Warts(2019-08-01) Pawalai Kraipeerapun; Somkid Amornsamankul; Ramkhamhaeng University; Mahidol University© 2019 IEEE. In this paper, complementary neural networks are applied to cascade generalization. Complementary neural networks comprise two neural networks trained to predict truth and falsity values. Two levels of cascade generalization are implemented in this paper. Two approaches are proposed. First, a neural network is trained in the base level whereas complementary neural networks are trained in meta level of cascade generalization. Second, complementary neural networks are trained in both levels of cascade generalization. The proposed methods are used to select cryotherapy method for wart treatment. The cryotherapy data set is obtained from UCI machine learning repository. Ten-fold cross validation is used in the experiment. The proposed approach gives 98.89% accuracy which higher than the existing methods which are cascade generalization and stacked generalization.Publication Metadata only Recursive axiomatizations for representable posets(2019-06-01) Rob Egrot; Mahidol University© 2019 World Scientific Publishing Company. We use model theoretic techniques and games to construct explicit first-order axiomatizations for the classes of posets that can be represented as systems of sets, where the order relation is given by inclusion, and existing meets and joins of specified countable cardinalities correspond to intersections and unions, respectively.Publication Metadata only Molecular diagnosis of solute carrier family 4 member 1 (SLC4A1) mutation-related autosomal recessive distal renaltubular acidosis(2019-02-01) Nipaporn Deejai; Suwannee Wisanuyotin; Choochai Nettuwakul; Sookkasem Khositseth; Nunghathai Sawasdee; Kiattichai Saetai; Pathai Yenchitsomanus; Nanyawan Rungroj; Khon Kaen University; Faculty of Medicine, Thammasat University; Faculty of Medicine, Siriraj Hospital, Mahidol University© American Society for Clinical Pathology 2018. All rights reserved. Background: Two common mutations of the solute carrier family 4 member 1 (SLC4A1) gene, namely, Southeast Asian ovalocytosis (SAO) and band 3 Bangkok 1 (G701D), cause autosomal recessive distal renal tubular acidosis (AR dRTA) in ethnic Southeast Asian populations. In this study, we applied the high-resolution melting (HRM) method for screening of AR dRTA associated with SLC4A1 mutations in 10 new patients with unknown cause(s) of AR dRTA. Methods: We analyzed SAO and G701D mutations in the patients and their family members using HRM. The results were confirmed by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) and DNA sequencing techniques. Results: All patients carried homozygous G701D mutation, whereas their family members had heterozygous G701D or homozygous wild-type. Conclusions: Homozygous G701D is a common cause of AR dRTA in ethnic Thai pediatric populations. HRM can be used as a rapid screening method for common SLC4A1 mutations that cause AR dRTA in Southeast Asian and other populations.Publication Metadata only Identifying design and requirement self-admitted technical debt using N-gram IDF(2019-03-05) Supatsara Wattanakriengkrai; Rungroj Maipradit; Hideki Hata; Morakot Choetkiertikul; Thanwadee Sunetnanta; Kenichi Matsumoto; Nara Institute of Science and Technology; Mahidol University© 2018 IEEE. In software projects, technical debt takes place when a developer adopting a trivial solution containing quick and easy shortcuts to implement over a suitable solution that can take a longer time to solve a problem. This can cause major additional costs leading to negative impacts for software maintenance since those shortcuts might need to be reworked in the future. Detecting technical debt early can help a team cope with those risks. In this paper, we focus on Self-Admitted Technical Debt (SATD) that is a debt intentionally produced by developers. We propose an automated model to identify two most common types of self-admitted technical debt, requirement and design debt, from source code comments. We combine N-gram IDF and auto-sklearn machine learning to build the model. With the empirical evaluation on ten projects, our approach outperform the baseline method by improving the performance over 20% when identifying requirement self-admitted technical debt and achieving an average F1-score of 64% when identifying design self-admitted technical debt.Publication Metadata only Description of a new species of Dermacentor Koch, 1844 (Acari: Ixodidae) from Laos and Thailand(2019-07-01) Dmitry A. Apanaskevich; Aummarin Chaloemthanetphong; Khamsing Vongphayloth; Arunee Ahantarig; Maria A. Apanaskevich; Paul T. Brey; Jeffrey C. Hertz; Khaithong Lakeomany; Ian W. Sutherland; Wachareeporn Trinachartvanit; Institut Pasteur du Laos; Zoological Institute of the Russian Academy of Sciences; Georgia Southern University; Mahidol University; U.S. Naval Medical Research Unit 2; Bureau of Medicine and Surgery© 2019, Springer Nature B.V. Dermacentor laothaiensis n. sp. (Acari: Ixodidae) is described based on adults ex wild boar and vegetation from Laos and Thailand. Adults of D. laothaiensis n. sp. are similar to those of D. bellulus (Schulze, 1935) and D. steini (Schulze, 1933) but can be distinguished by the conscutum shape, colour pattern of the conscutum and scutum, the density of punctations on the pseudoscutum and scutum and the shape of female genital structures.Publication Metadata only Effect of air-blowing time and long-term storage on bond strength of universal adhesives to dentin(2019-06-01) Pipop Saikaew; Jiale Fu; A. F.M.Almas Chowdhury; Ricardo M. Carvalho; Hidehiko Sano; Hokkaido University; Mahidol University; The University of British Columbia; China Medical University Shenyang© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Objectives: To evaluate the effects of air-blowing time and storage time on microtensile bond strength (μTBS) of universal adhesives to dentin. Materials and methods: Ninety flat dentin surfaces from extracted human third molars were bonded with three universal adhesives (Clearfil Universal Bond-CU; G-Premio Bond-GP; Scotchbond Universal Adhesives-SB). Bonded dentin surfaces were air-dried for 5 s, 15 s, or 30 s followed by resin composite built-up. Resin-dentin beams were tested with μTBS test after different storage time in distilled water (24 h and 1 year). Data were analyzed by three-way ANOVA and Duncan test at (α = 0.05). Failure mode and resin-dentin interfaces were observed using a scanning electron microscope (SEM). Specific features of fractured beams after μTBS were further observed using SEM at high magnification. Results: Extension of air-blowing time from 5 s to 30 s increased the 24 h μTBS of CU only. Bond strength of all adhesives significantly decreased after 1-year storage except for CU at 5 s and 30 s of air-blowing time. One-year μTBS were significantly higher when air-blowing times were extended to 15 s for SB and 30 s for CU. Air-blowing time had no influence on GP. Conclusion: The effect of air-blowing time and storage time on resin-dentin bond was material-dependent. Clinical relevance: Extended air-blowing time increased the bond strength and bond durability of CU. Extension of air-blowing time to 15 s and 30 s improve the long-term bond strength of SB and CU, respectively.Publication Metadata only Modeling the spread of porcine reproductive and respiratory syndrome virus (PRRSV) in a swine population: transmission dynamics, immunity information, and optimal control strategies(2019-12-01) Phithakdet Phoo-ngurn; Chanakarn Kiataramkul; Farida Chamchod; South Carolina Commission on Higher Education; King Mongkut's University of Technology North Bangkok; Mahidol University© 2019, The Author(s). Porcine reproductive and respiratory syndrome (PRRS) is an important swine disease that affects many swine industries worldwide. The disease can cause reproductive failure and respiratory problems in a swine population. As vaccination is an important tool to control the spread of PRRS virus (PRRSV), we employ a mathematical model to investigate the transmission dynamics of PRRSV and the effects of immunity information, as well as vaccination control strategies. We also explore optimal vaccination coverage and vaccination rate to minimize the number of infected swines and vaccination efforts. Our results suggest that: (i) higher vaccination coverage and vaccination rate together with prior knowledge about immunity may help reduce the prevalence of PRRSV, and (ii) longer maximum vaccination efforts are required when swines stay longer in a population and it takes them longer time to recover from PRRS infections.Publication Metadata only Transcriptional regulation of the Pseudomonas aeruginosa iron-sulfur cluster assembly pathway by binding of IscR to multiple sites(2019-01-01) Kritsakorn Saninjuk; Adisak Romsang; Jintana Duang-Nkern; Paiboon Vattanaviboon; Skorn Mongkolsuk; Chulabhorn Research Institute; Thailand Ministry of Education; Mahidol University; Chulabhorn Royal Academy© 2019 Saninjuk et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Iron-sulfur ([Fe-S]) cluster proteins have essential functions in many biological processes. [Fe-S] homeostasis is crucial for bacterial survival under a wide range of environmental conditions. IscR is a global transcriptional regulator in Pseudomonas aeruginosa; it has been shown to regulate genes involved in [Fe-S] cluster biosynthesis, iron homeostasis, resistance to oxidants, and pathogenicity. Many aspects of the IscR transcriptional regulatory mechanism differ from those of other well-studied systems. This study demonstrates the mechanisms of IscR Type-1 binding to its target sites that mediate the repression of gene expression at the isc operon, nfuA, and tpx. The analysis of IscR binding to multiple binding sites in the promoter region of the isc operon reveals that IscR first binds to the high-affinity site B followed by binding to the low-affinity site A. The results of in vitro IscR binding assays and in vivo analysis of IscR-mediated repression of gene expression support the role of site B as the primary site, while site A has only a minor role in the efficiency of IscR repression of gene expression. Ligation of an [Fe-S] cluster to IscR is required for the binding of IscR to target sites and in vivo repression and stress-induced gene expression. Analysis of Type-1 sites in many bacteria, including P. aeruginosa, indicates that the first and the last three AT-rich bases were among the most highly conserved bases within all analyzed Type-1 sites. Herein, we first propose the putative sequence of P. aeruginosa IscR Type-1 binding motif as 5’AWWSSYRMNNWWWTNNNWSGGNYWW3’. This can benefit further studies in the identification of novel genes under the IscR regulon and the regulatory mechanism model of P. aeruginosa IscR as it contributes to the roles of an [Fe-S] cluster in several biologically important cellular activities.Publication Metadata only Treatment outcome and prognostic factors in PCNSL(2019-06-13) Pimjai Niparuck; Paisarn Boonsakan; Taksayut Sutthippingkiat; Sulada Pukiat; Pichika Chantrathammachart; Sithakom Phusanti; Kochawan Boonyawat; Teeraya Puavilai; Pantep Angchaisuksiri; Artit Ungkanont; Suporn Chuncharunee; Vichai Atichartakarn; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Mahidol University© 2019 The Author(s). Objectives: Standard treatment with a thiotepa-based regimen in countries with a limited resource is less feasible. Aims of the study were to evaluate the treatment outcome, and identify the prognostic factors in patients with primary central nervous system lymphoma (PCNSL). Methods: We conducted a retrospective study of 43 patients diagnosed with PCNSL, DLBCL subtype, who were treated with either HDMTX-based regimen, whole brain radiotherapy (WBRT), or both between 2010 and 2017. Results: There were 43 patients with a median age of 65 years (range 34-89 years). Protein expression of CD10, Bcl6, MUM1, Bcl2 and MYC were found in 19, 86, 91, 91 and 23%, respectively. Both germinal center B cell (GCB) and double-expressor (MYC+/Bcl2+) lymphomas were found in 21%. Multiple brain lesions and maximum tumor diameter (MTD) ≥5 cm were seen in 27 and 10 patients, respectively. Chemotherapy combined with WBRT, chemotherapy and WBRT were given to 20, 14 and 9 patients, respectively. Overall complete remission (CR) rate was 55.8%. Those receiving a combined-modality therapy had a higher CR rate than those treated with either chemotherapy (75% versus 36%, p = 0.036) or WBRT (75% versus 44%, p = 0.109). Median follow-up time was 17 months, and a 7-year overall survival (OS) was 40%. Features associated with a prolonged OS were an ECOG score ≤ 2 (p = 0.001), multiple brain lesions (p = 0.010), multiple area of brain involvement (p = 0.023), MTD < 5 cm (p = 0.004), GCB subtype (p = 0.003) and positive CD10 staining (p = 0.007). Expression of Bcl2 protein was associated with a significantly worse OS in the non-GCB DLBCL patients. Discussion: The factors affecting treatment outcomes in PCNSL were cell of origin of DLBCL, lesion characteristics, patients' status and treatment regimen.