Scopus 2026
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Item Metadata only Anticoccidial Resistance in Eimeria spp. From Thai Broiler Farms Using Shuttle Programs(2026-01-01) Tongkamsai S.; Boobphahom S.; Apphaicha R.; Sreta D.; Limpavithayakul K.; Chansiripornchai N.; Tongkamsai S.; Mahidol UniversityCoccidiosis is a widespread poultry disease caused by Eimeria species and significantly affects performance and productivity. In the field, co-infections with multiple Eimeria species are commonly observed. While prophylactic coccidiostats are commonly used in feed for disease control, their extensive application has led to drug resistance, especially with the widespread use of shuttle programs in broiler production in Thailand. Understanding the resistance profiles of commonly used anticoccidials is crucial for developing effective prevention strategies. This study assessed the sensitivity of field isolates of Eimeria acervulina (E. acervulina), E. maxima, and E. tenella from broiler farms in Thailand using four anticoccidial sensitivity tests (ASTs). A dose-titration trial was conducted to determine effective inoculation doses, focusing on lesion induction and reduced weight gain while minimizing mortality. A total of 112-day-old broiler chicks were used to evaluate sensitivity to three widely used anticoccidial agents: narasin combined with nicarbazin, salinomycin, and maduramicin. Sensitivity assessment was conducted using four key indices: the anticoccidial index (ACI), the percentage of optimum anticoccidial activity (POAA), the reduction of lesion scores (RLS), and the relative oocyst production (ROP). During the study, broilers were fed the same combination of narasin + nicarbazin in the starter diet, while the in-feed anticoccidial in the grower phase was either maduramicin or salinomycin. Following inoculation with oral doses of sporulated oocysts of E. acervulina (2 × 105), E. maxima (1 × 105), and E. tenella (1.5 × 104), both medicated and infected-unmedicated groups showed significantly lower weight gain compared to uninfected controls (p < 0.05). While both salinomycin and maduramicin treatments significantly reduced intestinal lesion scores and fecal oocyst shedding compared with the infected-unmedicated group (p < 0.05), the reductions were incomplete. Notably, the calculated indices yielded discordant results: ACI and ROP values initially suggested sensitivity, whereas POAA and RLS indicated reduced efficacy. According to the study’s composite point system—where resistance in two indices signifies moderate resistance—the field isolates were classified as moderately resistant to both salinomycin and maduramicin. Histopathological analysis supported this, confirming that while medication reduced the severity of infection, it did not entirely prevent Eimeria colonization. This study provides the first systematic AST evaluation of Thai Eimeria isolates under shuttle-program conditions. The findings highlight the emergence of moderate drug resistance even within these structured programs, emphasizing a critical practical relevance for the poultry industry. To sustain long-term efficacy in Thai broiler production, there is an urgent need for strategic drug rotation and the integration of alternative control measures, such as vaccination and bio-shuttle strategies.Item Metadata only Impact of general anesthesia with vasopressor support on uteroplacental perfusion during fetal spina bifida repair(2026-01-01) Vergote S.; Van der Veeken L.; Van den Eede E.; Arai T.; Watananirun K.; Brenders A.; Deprest J.; Bleeser T.; Devroe S.; Van de Velde M.; Devlieger R.; van der Merwe H.; De Catte L.; Lewi L.; Russo F.; Van Calsteren K.; Lannoo L.; Vergote S.; Mahidol UniversityObjectives: To evaluate the combined effect of maternal general anesthesia and vasopressor support on uterine hemodynamics during the second trimester in pregnancies undergoing fetal spina bifida repair and to determine whether fetal spina bifida repair alters uteroplacental perfusion. Methods: This was a prospective single-center study conducted at the Fetal Medicine Unit of University Hospitals Leuven, Leuven, Belgium between August 2021 and April 2025. All participants underwent fetal spina bifida repair under general anesthesia with vasopressor support, titrated to maintain a mean arterial pressure ≥ 90% of the preoperative baseline value. Uterine artery (UtA) and umbilical artery Doppler measurements were obtained at six timepoints: (1) the day before surgery to establish preoperative baseline values; (2) immediately after epidural catheter placement, initiation of general anesthesia and intubation (at the initiation of noradrenaline); (3) 5 min after initiation of general anesthesia with vasopressor support; (4) at the end of surgery, after maternal skin closure and before extubation; (5) on postoperative day 1; and (6) on postoperative day 6. The primary outcome was the change in UtA pulsatility index (PI) 5 min after initiation of general anesthesia compared with the preoperative baseline value. Secondary outcomes included UtA blood flow estimated from time-averaged maximum velocity and vessel diameter and fetal umbilical artery Doppler parameters. Statistical analysis included paired t-tests, Wilcoxon signed-rank tests and mixed-effects models. Results: A total of 33 women undergoing fetal spina bifida repair were included, of whom 23 underwent open repair and 10 underwent fetoscopic repair. At the preoperative baseline, median UtA-PI was 0.77 (interquartile range (IQR), 0.64–0.95), median UtA resistance index (RI) was 0.52 (IQR, 0.45–0.59) and median UtA blood flow was 307.5 (IQR, 209.8–542.2) mL/min. At 5 min after the initiation of general anesthesia with vasopressor support, UtA-PI (median, 0.87 (IQR, 0.68–1.04)) and UtA blood flow (median, 310.7 (IQR, 199.0–421.4) mL/min) were comparable to baseline values (both P > 0.05). At this timepoint, umbilical artery PI was higher than the baseline value (P = 0.008) while fetal heart rate decreased. At the end of surgery, UtA-PI (median, 1.46 (IQR, 1.04–2.21)) and UtA-RI (median, 0.76 (IQR, 0.62–0.91)) were elevated compared with baseline values (both P < 0.001) and UtA blood flow was reduced (median, 182.1 (IQR, 79.8–351.5) mL/min; P = 0.031). Elevation of UtA-PI and UtA-RI persisted on postoperative days 1 and 6, but umbilical artery indices generally normalized back to preoperative baseline levels. There were no significant differences in UtA or fetal Doppler parameters between surgical techniques. Conclusions: General anesthesia with vasopressor support preserved uterine perfusion and blood flow without increasing vascular resistance. During fetal spina bifida repair, UtA resistance increased and UtA blood flow decreased. These parameters did not normalize back to preoperative baseline values within 6 days after surgery. In contrast, perioperative fetal Doppler changes resolved immediately postoperatively © 2026 International Society of Ultrasound in Obstetrics and Gynecology.Item Metadata only Association between quadriceps muscle strength and ultrasound-derived echogenicity in individuals with patellofemoral pain syndrome: A cross-sectional study(2026-07-01) Kantiya A.; Fungkiatphaiboon P.; Praoprirtpirom K.; Tangsriwong K.; Wang H.K.; Wattananon P.; Kantiya A.; Mahidol UniversityBackground Patellofemoral pain syndrome (PFPS) is commonly associated with quadriceps weakness and altered knee mechanics. While muscle quantity is frequently assessed, the role of muscle quality, indirectly represented by ultrasound-derived echogenicity, remains unclear in this population. This study aimed to compare quadriceps strength between individuals with PFPS and healthy controls (CON) and to explore whether quadriceps strength is associated with rectus femoris echogenicity, age, BMI, and knee function. Methods A cross-sectional study was conducted with 55 participants with PFPS and 43 healthy controls. Rehabilitative ultrasound imaging was used to measure rectus femoris echogenicity at rest and during contraction at 5% body weight. Quadriceps strength was assessed using hand-held dynamometry, and knee function was evaluated using the Knee Injury and Osteoarthritis Outcome Score (KOOS). Results The PFPS group demonstrated significantly lower quadriceps strength than the CON group ( P = 0.045). In the PFPS group, quadriceps strength was significantly and inversely correlated with resting echogenicity ( r = −0.284, P = 0.035), contraction echogenicity ( r = −0.296, P = 0.028), and age ( r = −0.381, P = 0.004). Quadriceps strength was positively correlated with KOOS scores ( r = 0.434, P = 0.001). However, after controlling for age and BMI, the associations between quadriceps strength and resting echogenicity ( r = −0.167, P = 0.233) and contraction echogenicity ( r = −0.173, P = 0.215) were attenuated and no longer statistically significant. No significant correlations were observed in the CON group. Conclusion Individuals with PFPS demonstrated reduced quadriceps strength. Rectus femoris echogenicity may provide complementary information regarding muscle quality, but its association with strength appears to be partly influenced by age and BMI.Item Metadata only Natural Rubber-Based Soft Robotic Gripper with Tunable Performance(2026-05-22) Sriring M.; Watcharakan T.; Meesaringkarn S.; Sriring S.; Khanchaitit P.; Sirisinha C.; Sriring M.; Mahidol UniversityNatural rubber (NR) offers a sustainable and mechanically robust alternative to synthetic elastomers for soft robotics, but its broader adoption has been limited by challenges in fabricating the intricate geometries required for advanced actuator architectures. In response, we employ a gelling-assisted latex casting process with 3D-printed molds to reliably fabricate NR-based actuators with varied geometric parameters and systematically investigate their actuation behaviors. Under pneumatic actuation, bending efficiency and sensitivity of the actuator increased with chamber number and height, resulting in enhanced gripping performance, whereas increased chamber wall thickness suppressed actuation and grasping capability. To assess the influence of material stiffness, actuators fabricated from NR with different cross-link densities (3.26 × 10–5, 5.62 × 10–5, and 6.94 × 10–5 mol/g) were further examined. Actuators with lower cross-link density exhibited greater bending ability and higher sensitivity at low actuation pressures, whereas higher cross-link density enhanced mechanical stiffness and burst resistance, enabling stable operation at elevated pressures. As a result, actuators with low, medium, and high cross-link densities achieved maximum gripping forces of 10.85, 11.99, and up to 18.25 N at actuation pressures of 125, 150, and 200 kPa, respectively. These findings demonstrate that the actuation performance of NR-based soft robotics can be precisely tailored through the synergistic optimization of geometric design and rubber network structure. Finally, a four-arm soft gripper assembled from the optimized NR actuators effectively manipulated a range of delicate objects, highlighting the potential of NR as a sustainable and versatile material for next-generation soft robotic systems.Item Metadata only Prioritizing multi-stakeholder solutions for autonomous delivery robot implementation in urban last-mile logistics: An integrated socio-technical systems and institutional theory framework(2026-06-01) Sumrit D.; Apiwatvaravong S.; Sumrit D.; Mahidol UniversityAutonomous delivery robots (ADRs) are widely promoted as a solution to improve efficiency in urban last-mile logistics, yet their large-scale adoption remains limited. This is mainly due to social, regulatory, and infrastructure-related challenges rather than technological constraints. This study develops a structured decision-making framework to analyze ADR implementation barriers and to identify effective multi-stakeholder solutions while accounting for uncertainty and interdependencies in expert judgments. The results show that ADR barriers are highly interconnected. Institutional arrangements and infrastructure conditions act as underlying drivers that influence many other challenges, whereas public acceptance, cybersecurity concerns, and human–robot interaction issues largely emerge as outcomes of weak governance and limited system readiness. Safety compliance and risk liability are identified as the most influential barriers, followed by cybersecurity vulnerabilities and cultural or behavioral constraints. These findings indicate that progress in ADR adoption depends more on clear responsibilities, trust, and social readiness than on further technological development alone. The solution analysis shows that governance- and infrastructure-oriented actions are more effective than isolated technical measures. Ethical guidelines, clear legal frameworks, and stronger industry collaboration are identified as the most effective strategies, highlighting the need for coordinated action among regulators, technology developers, and logistics service providers. Theoretically, the study reinforces the view that ADRs should be understood as part of a broader urban system rather than as standalone technologies. Practically, the findings provide clear guidance for stakeholders seeking to enable responsible and scalable ADR deployment in urban last-mile logistics.Item Metadata only Taming the wild West: a systematic review and evaluation of bibliometric reviews of educational research(2026-01-01) Hallinger P.; Narong D.K.; Hallinger P.; Mahidol UniversityBibliometric reviews have proliferated across academic disciplines in recent years. This unprecedented publication trajectory has prompted concerns about quality and the contributions of these reviews to knowledge. This systematic review used descriptive statistics and content analysis to review 1,873 bibliometric reviews of educational research published in Scopus-indexed journals through 2024. The authors analyzed the research landscape, topical trends, and methodological quality of these education reviews. Remarkably, 87% percent were published between 2020 and 2024, and 97% since 2015. A majority were published in Scopus Q1 and Q2 journals, including 49 in top 1% journals by field. Also surprising, 69% of the reviews were authored by scholars in emerging nations. This contrasts with original research publications, which have traditionally been dominated by authors from economically developed societies. The most frequently studied topics concerned higher education, research performance, and technology-enabled learning. Further analysis found that numerous occurrences of authors conducting reviews on the same topic, using similar methods, over the same time span, and without cross-referencing. A methodological quality rubric was applied to a stratified random sample of 60 bibliometric reviews published in high-, mid-, and low-percentile Scopus journals. Although these analyses identified some exemplary reviews, the broader results indicated that many bibliometric reviews failed to realize their potential due to persistent methodological weaknesses and a lack of conceptual depth. These deficits threaten the quality and contribution of bibliometric reviews in education and other disciplines. Specific recommendations are made to improve the quality and contributions of future bibliometric reviews.Item Metadata only Plant-based production and characterization of monoclonal antibodies targeting extracellular enveloped proteins of the Mpox Virus(2026-09-01) Jirananon K.; Shanmugaraj B.; Manopwisedjaroen S.; Thitithanyanont A.; Teengam P.; Phoolcharoen W.; Jirananon K.; Mahidol UniversityThe recent mpox outbreaks in non-endemic countries highlight the urgent need for improved therapeutics and diagnostics. In this study, monoclonal antibodies (mAbs) targeting the mpox enveloped virion antigens A35 and B6 were transiently expressed in Nicotiana benthamiana using a geminiviral vector system. Following agroinfiltration, anti-A35 and anti-B6 mAbs accumulated to 27 µg/g fresh weight at 3 days and 260 µg/g at 7 days post-infiltration, respectively. SDS-PAGE and Western blot analyses confirmed the assembly of antibodies, and the purified antibodies bound to mpox-infected Vero cells. The neutralization assays demonstrated moderate reductions in viral infection under the tested conditions. Additionally, an electrochemical immunosensor demonstrated the ability of plant-produced antibodies to detect mpox virus through antigen–antibody binding induced current changes. These results support that the plant-based systems as rapid platforms for producing mpox-specific antibodies for diagnostics and antiviral research.Item Metadata only Ageing in Place in Thailand: Development of a Composite Index for the Older Population(2026-01-01) Hattapradit F.; Punpuing S.; Tangchonlatip K.; Katewongsa P.; Sawangdee Y.; Chuanwan S.; Hattapradit F.; Mahidol UniversityThis study developed a version of the ageing in place index for the older population in Thailand. using secondary data from the National Statistical Office’s surveys of the older persons in Thailand conducted in 2021. Data analysis includes descriptive statistics of the sample population and analysis of the index using the Aggregate Z score method to determine the sum of the index of each component. The study found that the ageing in place index, adapted from the concept of ageing in place, consists of five dimensions (place, social networks, support, technology, and personal characteristics) and 16 sub-indicators. The composite index development process involved seven steps. The ageing in place index tool passed a content-validity check, achieving a score of the Index of item objective congruence, interrater reliability. The final overall score of the ageing in place index is interpreted into three levels: low (0.000-0.499), moderate (0.500-0.799), and high (0.800-1.000). The values for the ageing in place index were compared to the established standard target indicators in order to inform policy planning for ageing in place and improve the quality of life for the older population of Thailand.Item Metadata only Gene–disease relationships for glomerular phenotypes: expert recommendations from ClinGen(2026-01-01) Byrne A.B.; Li A.S.; Chung E.Y.M.; Edoh E.; Dziadzio H.; Ajuyah P.; Ars E.; Caliskan Y.; Dirim A.B.; Elliott M.D.; Gupta A.; Jayasinghe K.; Mallett A.J.; Ratliff J.C.; Sampson M.G.; Savige J.; Schlondorff J.S.; Stark Z.; Webb R.F.; Wilson P.C.; Wongboonsin J.; van Eerde A.M.; Li A.S.; Kim L.; Hoefele J.; Gbadegesin R.; Chung E.Y.M.; Bierzynska A.; Aypek H.; Pollak M.R.; McCarthy H.J.; Quinlan C.; Lennon R.; Byrne A.B.; Mahidol UniversityGlomerular diseases are complex conditions, many of which have a genetic basis. However, although some genetic variants can affect glomerular and thereby kidney function, not all identified variants are pathogenic. The process of evaluating genetic and experimental evidence to determine the validity of gene–disease relationships is known as gene curation, and it is critical for the identification of genes that should be examined in diagnostic tests and used to guide clinical management. Gene curation is a key role of the Clinical Genome Resource (ClinGen) and aims to ensure that the evidence underlying asserted gene–disease relationships across a range of diseases is sufficiently robust through comprehensive review of evidence and standardized evaluation by genetic and disease area-specific experts. The ClinGen Glomerulopathy Gene Curation Expert Panel has evaluated 57 gene–disease relationships from 56 genes that have been putatively linked to glomerular phenotypes. This evaluation identified 34 genes that reached a definitive level of evidence for gene–disease clinical validity. Ten genes had moderate supporting evidence and 11 had limited supporting evidence. A further two genes had insufficient evidence for any clinically valid relationship to disease. This curation establishes a comprehensive framework for the ongoing assessment of gene–disease relationships and provides a valuable reference for diagnostic genetic testing panels that target glomerular disease.Item Metadata only A Review of Epidemiological Modeling Studies on Monkeypox(2026-05-15) Li J.; Luo Q.; Beltran E.; Chen Q.; Perez S.; Frutos R.; Su Y.; Liu Z.; Rui J.; Chen T.; Luo K.; Zhao Z.; Li J.; Mahidol UniversityBetween 2022 and 2025, nearly 130,000 confirmed monkeypox (mpox) cases, including over 280 deaths, were reported to the World Health Organization (WHO) from 130 countries and territories, prompting the WHO to declare it a public health emergency of international concern on two occasions. Hence, to enable a scientific approach to prevention and control, transmission dynamics through mathematical modeling must be elucidated. Through a comprehensive literature review on the modeling of mpox transmission dynamics, this study indicates that existing research primarily extends the susceptible-infectious-recovered (SIR) modeling framework and largely focuses on the 2022 global outbreak. The analysis revealed significant variations in mpox virulence, particularly dependent on the subtypes, and variations according to the descending order of clades: Ib>Ia>IIa>IIb. Interpersonal transmission capacity was notably higher for clade II than for clade I, highlighting geographical disparities, with the highest transmission capacity in the Americas, moderate in Europe and Oceania, and the lowest in Asia. In contrast, Africa maintained consistently low but non-declining transmission levels. Furthermore, the study confirmed significant co-infection patterns between mpox and sexually transmitted diseases, such as Human Immunodeficiency Virus (HIV) and syphilis, along with evidence of synergistic transmission interactions among multiple pathogens. This study provides crucial modeling evidence for mpox regulation. By quantifying high-risk populations, evaluating intervention effectiveness, and identifying the risks associated with subtypes and geographic areas, this study offers a comprehensive reference for understanding the interface between model complexity and practical application. These advancements have enhanced the strategic focus and precision of public health responses during outbreaks.Monkeypox (mpox) is a zoonotic infectious disease caused by the monkeypox virus (MPXV), with clinical manifestations similar to those of smallpox but with lower incidence and mortality (1). MPXV is classified into clades I (formerly the Central African or Congo Basin clade, subdivided into Ia and Ib) and II (formerly the West African clade, subdivided into IIa and IIb). Transmission occurs via direct contact with infectious lesions, body fluids, respiratory droplets, or contaminated fomites (2). From 1970 to 2017, mpox was endemic primarily in Central and West Africa, predominantly via animal-to-human transmission. Sporadic cases in non-endemic regions were largely related to travel or animal importation (3−4). However, human-to-human transmission has increased following the 2017 Nigerian outbreak (5). In May 2022, a large global outbreak occurred, spreading primarily through sexual contact networks (6), with cases reported among men who have sex with men (MSM). The epidemiological modeling framework for mpox has evolved over the decades, with research on transmission dynamics among humans and between humans and animals, forming a relatively complete theoretical system. This study was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews guidelines (7). The literature search covered the China National Knowledge Infrastructure (CNKI), Web of Science, and PubMed for the period spanning 3 years following the global spread of mpox in 2022 (2022–2025). The focus was on the literature employing mathematical modeling methods to study the transmission dynamics models of orthopoxvirus (mpox) among humans or between humans and animals. The searches were conducted on December 25, 2025, using keywords including "mpox," "Ia/Ib/IIa/IIb," "dynamic" and "model." "Monkeypox" was also included as a keyword for the 2022 publications to account for the WHO nomenclature change in November 2022. This study did not include gray literature, and all analyses were based on published peer-reviewed academic literature. No geographic or language restrictions were applied. The systematic search yielded 11,824 relevant records. After deduplication, 5,479 duplicate records were removed. The remaining records were independently screened by two reviewers in a two-round process and discrepancies were resolved by consensus. The first round, based on title and abstract review assessing the application of mathematical compartmental models, identified 122 articles for full-text assessment. During the second round of full-text screening, 39 articles that did not analyze the epidemic transmission potential and 4 dissertations were excluded. Ultimately, 79 studies were included in the systematic review of compartmental structures, parameter settings, and dynamic characteristics of the models (Figure 1).Item Metadata only Self-Powered Microfluidic Device with Laser-Converted Graphene Electrodes for Immobilization-Free Electrochemical Detection of MPOX Virus DNA via Mismatch-Driven Nanocomplexes(2026-01-01) Sapyen W.; Ruecha N.; Weeranoppanant N.; Khositanon C.; Pasomsub E.; Wu H.; Yakoh A.; Sapyen W.; Mahidol UniversityThe re-emergence of monkeypox virus (MPXV) underscores the urgent need for rapid and decentralized diagnostic tools. Herein, we present an innovative, immobilization-free, and label-free paper-based electrochemical fast-flow microfluidic device (eFMD) integrated with near-field communication (NFC) technology for point-of-care detection of MPXV. The platform utilizes a C─C mismatch-mediated Ag+ intercalation mechanism, generating distinct current shifts detectable via differential pulse voltammetry (DPV). A microfluidic device with optimized serpentine mixing channels ensures efficient Ag+ intercalation without external power sources. The biosensor incorporates laser-converted graphene (LCG) electrodes, offering eco-friendly, scalable, and solvent-free fabrication with rapid prototyping and high design flexibility, ideal for disposable diagnostics. Key assay parameters including buffer type, mismatch probe concentration, AgNO3 levels, and detection time were systematically optimized to enhance sensitivity. The biosensor demonstrated excellent analytical performance with a low detection limit down to 1.4 pм for MPXV DNA and significant discrimination against single-base mismatched sequences. Robust performance was further demonstrated in complex matrices, including spiked biological samples and clinically relevant lesion swab specimens, with detection sensitivity down to 1.3 copies mL−1 and complete concordance with PCR classification. Importantly, the system allows for wireless, on-site readout via smartphones within 35 min, making it suitable for rapid MPXV screening.Item Metadata only AAV-mediated delivery of CRISPR/Cas9 targeting conserved overlapping ORFs efficiently suppresses HBV replication in hepatocyte models(2026-09-01) Kongsomboonchoke P.; Pewkliang Y.; Thongsri P.; Tubsuwan A.; Bhukhai K.; Asavapanumas N.; Phanthong P.; Borwornpinyo S.; Chiangjong W.; Sa-ngiamsuntorn K.; Hongeng S.; Kongsomboonchoke P.; Mahidol UniversityChronic hepatitis B virus (HBV) infection remains a major global health burden due to the persistence of covalently closed circular DNA (cccDNA), which limits current antiviral therapies. We developed an adeno-associated virus (AAV)-delivered CRISPR/Cas9 system targeting conserved regions of the HBV genome. Three guide RNAs (gRNA1–3) targeting overlapping open reading frames of the surface antigen and polymerase genes were evaluated in HepG2.2.15 cells and HBV-infected hepatocyte-like cells (imHCs), with a reverse transcriptase-targeting gRNA and tenofovir alafenamide as controls. All gRNAs significantly reduced intracellular and extracellular HBV DNA levels and moderately decreased HBsAg secretion. Notably, gRNA2 induced a frameshift mutation and demonstrated superior antiviral efficacy, markedly reducing cccDNA levels, viral DNA levels, viral RNA levels, HBcAg expression, and HBsAg secretion with suppression maintained for up to 12 days. These findings highlight AAV-mediated CRISPR/Cas9 as a promising gene-based therapy for chronic HBV infection.Item Metadata only Unique molecular profiling of monocyte responses to a high dose of DENV-NS1 reflected the effect of NS1 on hemostasis(2026-12-01) Saisingha K.; Kaofai C.; Modhiran N.; Watterson D.; Paiboonpol S.; Pawaputanun A.; Leaungwutiwong P.; Hanboonkunupakarn B.; Mansanguan C.; Limthongkul J.; Ponpuak M.; Jearanaiwitayakul T.; Ubol S.; Saisingha K.; Mahidol UniversityThe pathogenesis of dengue is complex and requires further study. Infection with the dengue virus can lead to severe dengue, in which patients experience severe plasma leakage and excessive bleeding. The dengue virus non-structural protein 1 (DENV NS1) is among several factors that affect the outcome. The secreted form of this protein induces severe cases through direct and indirect immunopathogenesis mechanisms. Previously, we discovered that the stimulation of blood monocytes by NS1 upregulates transcripts involved in inflammation, and suppresses transcripts involved in antiviral processes. In this study, we examined the specific responses unique to high-dose NS1 exposure of cultured human monocytes. We found that genes involved in hemostasis were altered following NS1 exposure and were associated with platelet activation, fibrinolysis, and hemostatic regulation. Some of these expression changes were also observed in patients with dengue fever (DF) or dengue hemorrhagic fever (DHF). The altered expression of these genes suggests that fibrinolysis is more likely balanced during DF, whereas coagulation may be impaired in DHF.Item Metadata only Situational Formation of Enduring Traits: A Comprehensive Review of the Hierarchical Nature of Self-Regulated Learning(2026-12-01) Akamatsu D.; Kakinuma K.; Fujiwara T.; Akamatsu D.; Mahidol UniversitySelf-regulated learning (SRL) researchers acknowledge both top-down influences of dispositions and bottom-up influences of situational learning processes. However, the hierarchical–bidirectional influence linking dispositions and SRL behaviors has not been systematically theorized. Existing SRL frameworks (e.g., MASRL) characterize monitoring and control processes in detail, but researchers tend to treat dispositions as largely static rather than as outcomes shaped through SRL engagement. To address this gap, we propose the Hierarchical–Bidirectional Model of SRL, which synthesizes (a) a hierarchical structure spanning general, academic, subject, and task/situation levels; (b) bidirectional dynamics between dispositions and SRL processes; and (c) domain similarity, which moderates cross-domain transfer and the strength of hierarchical links. We specify two central mechanisms. First, self-regulation in specific domains is influenced by proximal dispositions within those domains (Specification effect). Second, situational self-regulation fosters dispositions at corresponding levels, and this feedback loop contributes to the development of more resilient and general dispositions, which may extend beyond academic domains (Generalization effect). To empirically test these hierarchical–bidirectional relations, we highlight methodological approaches including intensive longitudinal designs, models that incorporate both lagged and contemporaneous effects, and psychological network analyses. We also emphasize the role of computational modeling for achieving clearer construct specification and for formally capturing the dynamic processes through which SRL cycles accumulate into dispositional development. Overall, we present a model that reframes SRL as a developmental, hierarchical, and reciprocal process, providing a unifying framework that integrates diverse constructs and clarifies how dispositions both guide and emerge from SRL across domains.Item Metadata only Structural strength analysis of the adjustment for seat height, leg rest angle, and backrest angle to design a wheelchair(2026-05-15) Keangin P.; Duangpool K.; Chawengwanicha P.; Srimuang W.; Keangin P.; Mahidol UniversityA wheelchair is a mobility aid usually designed to improve movement, facilitate daily activities, and enhance the quality of life for individuals with walking difficulties, particularly those with paralysis or elderly individuals. If a wheelchair lacks sufficient features, its functionality can be inadequate, leading to inefficient use and discomfort for the user. Conducting a strength analysis, particularly focusing on stress distribution within the structural design, can aid recommend wheelchair designs that effectively support users in various scenarios. This research aims to perform a strength analysis to inform the design of a wheelchair intended for medical applications. The study examines the effects of variations in seat height (34 cm, 50 cm, and 66 cm), leg rest angle (30 degrees, 60 degrees, and 90 degrees), and backrest angle (30 degrees, 60 degrees, and 90 degrees) on wheelchair design. The details regarding idea design and strength analysis, namely von Mises stress and displacement distributions, of various scenarios are analyzed using three-dimensional finite element wheelchair modeling. The simulation results indicate that the highest maximum von Mises stress observed in all situations does not exceed the material's yield strength. The backrest angle of the 90 degrees' case is found to have a maximum von Mises stress. The study's findings could serve as an appreciated foundation for establishing guidelines for wheelchair design and other medical devices aimed at supporting individuals with disabilities and other kinds of elderly care.Item Metadata only Marine mollusk shells as natural bone substitutes: Surface characterization and osteogenic activities(2026-01-01) Charoonpatrapong K.; Panyayong W.; Chuenarrom C.; Charoonpatrapong K.; Mahidol UniversityBone substitutes from various sources have been used to facilitate bone regeneration. In this study, we performed a comparative analysis of the effects of marine mollusk shells and their bioactivities on osteoblasts and osteoclasts. The results showed that the surfaces of A. granulosa and C. belcheri shells had higher surface roughness than that of P. viridis. Different surface morphologies but similar elements were found on the surfaces of all shell groups, and additional elements, particularly silicon, were found on the surface of A. granulosa. After growing osteoblasts on the shells for 4–7 days, C. belcheri, P. viridis, and A. granulosa shells had significantly more cells than P. undulata shells. Similar osteoblast differentiation was observed in all shell groups after 7–14 days. All shell groups, particularly C. belcheri and P. viridis, favored osteoclast formation. This study suggests the potential use of mollusk shells to develop alternative biocompatible bone-substitute materials.Item Metadata only Pain, Clinical Tests, and Disability in Individuals With Radicular Low Back Pain: A Responsiveness and Correlation Study(2026-01-01) Halidadjiyah; Sakulsriprasert P.; Chansirinukor W.; Halidadjiyah; Mahidol UniversityBackground: This study aimed to investigate the responsiveness of pain, clinical tests, and disability in individuals with RLBP. Methods: Thirty-two patients with RLBP were assessed for pain intensity (at rest and at worst), clinical tests comprising straight leg raising (SLR) and five-meter walk test (5MWT), and disability was assessed by the Modified Oswestry Disability Index (MODI) at baseline, immediately after, 1 week, and 2 weeks after first-session interventions. Change scores and effect size (ES) were calculated. Results: Pain at rest and pain at worst were most responsive with large ES, especially at 2 weeks (ES = 3.6 and 3.6, respectively). SLR and 5MWT were responsive with large ES at 2 weeks (ES = 0.9 and 0.9, respectively). Also, MODI was responsive at 2 weeks (ES = 1.0). Conclusions: This study indicated that the responsiveness of pain intensity (at rest and at worst), SLR, 5MWT, and MODI in patients with RLBP at 2 weeks after therapeutic interventions was evident.Item Metadata only Vaccine-induced mouse antibodies targeting Plasmodium falciparum PfVFT antigen inhibit blood stages through multiple mechanisms(2026-12-01) Goh Y.S.; Mao H.; Hor P.X.; Loh C.Y.; Chang Z.W.; Hermsen C.C.; Nosten F.; Sauerwein R.W.; Rénia L.; Goh Y.S.; Mahidol UniversityWhile vaccines are central to eradicate malaria, they remain elusive, with numerous malaria vaccine candidates showing limited efficacy in Phase II and III studies. Controlled human malaria infection studies have showed that human volunteers, immunized with Plasmodium falciparum sporozoites under drug cover, were protected experimentally from a subsequent challenge. Here, to identify new targets associated with protection, we utilized a previously developed screening approach, where we screened sera from protected and non-protected individuals against newly included hypothetical antigens in a P. falciparum antigen library. PfVFT1 was found to be associated with protection, with antibodies against PfVFT1 being detected in all protected individuals. We found that vaccine-induced mouse anti-PfVFT sera inhibited parasite reinvasion into RBCs, promoted complement deposition to induce parasite lysis, and supported phagocytosis and antibody-dependent cellular inhibition of the parasite. Together, these data indicate that PfVFT1-specific antibodies can engage multiple effector mechanisms relevant to antimalarial immunity.Item Metadata only Awake Endoscopic Radiofrequency Ablation for Lateral Atlantoaxial Joint Pain(2026-01-01) Santipas B.; Chavalparit P.; Kim J.S.; Santipas B.; Mahidol UniversityChronic pain originating from the lateral atlantoaxial (C1–C2) joint is a challenging condition with limited long-term treatment options. Conventional radiofrequency ablation (RFA) techniques rely on indirect guidance, which can lead to incomplete nerve denervation and variable outcomes. This report introduces awake endoscopic radiofrequency ablation (ERFA) as a novel, minimally invasive treatment, complemented by advanced diagnostic imaging. We present the case of a 67-year-old male with intractable occipital and posterior neck pain refractory to previous surgeries. Single-photon emission computed tomography (SPECT/CT) was crucial in identifying active inflammation localized to the left C1–C2 facet joint as the primary pain generator. The patient then underwent an awake ERFA procedure targeting the C1–C2 and C2–C3 facet joints. The endoscopic approach provided direct visualization of the target areas, allowing for precise and thorough ablation. The awake setting enabled real-time patient feedback, confirming immediate symptom relief of 80% upon completion. This case highlights that the combination of SPECT/CT for accurate diagnosis and awake ERFA for precise, visually-confirmed denervation presents a promising and effective modality for managing this condition, potentially offering more durable relief than conventional techniques.Item Metadata only Virus-like particles as antiviral strategies against shrimp viral diseases(2026-09-01) Jariyapong P.; Pooljun C.; Weerachatyanukul W.; Jariyapong P.; Mahidol UniversityViral diseases continue to threaten the sustainability of shrimp aquaculture, prompting the urgent development of innovative antiviral strategies. Virus-like particles (VLPs), self-assembled viral capsid structures lacking infectious genomes, have emerged as well-established nanoplatforms with potential applications in molecular delivery and antiviral intervention in aquatic species. This systematic review synthesizes current evidence on the design and biophysical properties of VLP-based platforms in shrimp. Literature searches were conducted in PubMed and Scopus between December 2025 and February 2026, and studies investigating VLP- or capsid-based applications in shrimp models with antiviral-related outcomes were included. Nineteen studies met the inclusion criteria. Most studies focused on penaeid shrimp, particularly Penaeus vannamei , and primarily targeted white spot syndrome virus (WSSV). The majority of VLP constructs were derived from shrimp viral capsid proteins and produced in Escherichia coli , and were subsequently used to encapsulate dsRNA targeting viral structural genes. Across in vivo challenge models, 63.16% of studies reported improved survival or reduced mortality compared with controls, while 47.37% demonstrated reduced viral load following VLP-based treatment. Although heterogeneity in construct design and experimental conditions limits direct comparison, current evidence supports the potential of VLP platforms as multifunctional antiviral systems in shrimp. Standardized evaluation frameworks and field-relevant validation will be essential to advance their translational application in aquaculture.