Journal Issue: JAAS Vol. 11 No. 2
Issued Date
2561
Resource Type
Language
tha
eng
eng
File Type
application/pdf
Access Rights
open access
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ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหิดล
Journal Volume
JAAS Volume 12
(2562)
Articles
Effect of Sex on Growth Curve, Production Performance and Carcass Quality of Cherry Valley Ducks
(2018) Chanwit Kaewtapee; Kriengkrai Prahkarnkaeo; Chaiyapoom Bunchasak; Kasetsart University. Faculty of Agriculture. Department of Animal Science; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health
The objective of this study was to determine the effect of sex dimorphism on growth curve, production
performance and carcass quality of Cherry valley ducks. Fifteen male and fifteen female ducks were randomly
kept in an individual metabolic cage under evaporative cooling system. Ducks were fed with a starter-1 diet
(22.00% crude protein [CP], and 2,850 metabolizable energy [ME] kcal/kg) from 1 to 9 days, a start-2 diet
(20.00% CP and 2,900 ME kcal/kg) from 10 to 16 days, a grower diet (18.50% CP and 2,900 ME kcal/kg) from 17 to 42 days, and a finisher diet (16.99% CP and 2,950 ME kcal/kg) from 43 to 49 days. Water and feed were offered ad libitum throughout experimental period. The Gompertz model was used to develop the growth curve for male and female ducks. A two-sample t-test was used to compare the effect of sex dimorphism on production performance and carcass quality. The results showed that there were significant differences (P<0.05) in body
weight (BW), average daily gain and feed intake between male and female ducks from 42 days. The accuracy
of R2with Gompertz equations was 0.9907 and 0.9857 for male and female ducks, respectively. For carcass
quality and internal organs at 49 days of age, male ducks had greater skeleton, breast meat, feet and gizzard
(P<0.05), whereas female tended to have greater (P=0.10) skin with subcutaneous fat. In conclusion, since the
sex dimorphism of ducks showed significant effect on growth rate from 42 days of age, the separate sex growing should be considered to obtain the uniformity of BW and carcass quality of Cherry Valley ducks.
Effects of scabraside D on cell viability inhibition and apoptosis promotion of human hepatocellular carcinoma
(2018) Rassameepen Phonarknguen; Thanakorn Rawangchue; Orathai Thongjuy; Kanjana Assawasuparerk; รัศมีเพ็ญ โพธิ์นาคเงิน; ธนกร ระวังชื่อ; อรทัย ทองจุ้ย; กาญจนา อัศวศุภฤกษ์; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Disease in Wildlife and Exotic Animals; Mahidol University. Faculty of Veterinary Science. The Center for Veterinary Diagnosis; Mahidol University. Faculty of Veterinary Science. Department of Pre-Clinical and Appiled Animal Science
Scabraside D, derived from the sea cucumber Holothuria scabra is a sulfated triterpene glycoside which
possess in various biological activities. We assessed the activity of scabraside D and their effects on cell viability
and apoptosis on human hepatocellular carcinoma (HepG2) cell lines by MTT assay and staining with Hoechst
33342. The 25 to 100 μg/mL dose of scabraside D significantly decreased the viability of HepG2 cells, in a
dose-dependent manner. The treatment with scabraside D at dose 50 and 100 μg/mL significantly also induces
morphological changes of apoptotic cell, including cell shrinkage, nuclear chromatin condensation and
nuclear fragmentation. Quantitative real-time PCR shows that scabraside D up-regulated Bax and Caspase-3
while down-regulated Bcl-2 expressions in the HepG2 cells in dose-dependent manner. In conclusion, scabraside
D can inhibit cell viability and induce apoptosis in HepG2 cells. This study show that scabraside D may be used
as a new therapeutic agent for human hepatocellular carcinoma
Aqueous extract of Thai medical Herbs (Phytoplex) Inhibits Cell Proliferation and Induces Apoptosis in human cervical cancer cell line (HeLa cells)
(2018) Somjit Chaiwattanarungruengpaisan; Warunya Chakritbudsabong; Nattapat Rutjanavate; Rassameepen Phonarknguen; Ganokon Urkasemsin; Sasitorn Rungarunlert; สมจิตร ใช้วัฒนรุ่งเรืองไพศาล; วรัญญา ชาคริตบุษบง; ณัฐภัทร รุจจนเวท; รัศมีเพ็ญ โพธิ์นาคเงิน; กนกนอร เอื้อเกษมสิน; ศศิธร รุ่งอรุณเลิศ; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE); Mahidol University. Faculty of Veterinary Science. Department of Preclinic and Applied Animal Science; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Health
Cervical cancer remains a leading cause of cancer mortality in females. Chemotherapy is important as it
was a part of the main treatment for this type of cancer. However, multidrug resistance and serious side effects
have been major problems in cervical cancer chemotherapy. Therefore, the search for new anticancer drugs
from the native medicinal herbs, is very attractive. The combination extract of eight Thai medicinal herb recipes,
namely Phytoplex, is a commercial product of The Government Pharmaceutical Organization, has anti-cancer
effect on hepatocellular carcinoma cells (HepG2) in vitro. However, the anticancer effects and molecular
mechanisms of Phytoplex on cervical cancer have not yet been studied. The aim of this study was to evaluate
the inhibitory effect of Phytoplex on human cervical adenocarcinoma cells (HeLa cells) in vitro. HeLa cells were
treated with low concentrations of Phytoplex (50, 100, 500, 1,000 μg/ml) and high concentrations of Phytoplex
(2,000 and 5,000 μg/ml) compared with a positive control (0.1% mitomycin C) and a negative control (0 μg/ml of
Phytoplex) for 24, 48 and 72 h. Then, cell viability was evaluated using an MTT assay. The activities of caspase-3
(apoptosis marker) and Ki-67 (proliferation marker)†were investigated using an immunofluorescence assay. At
24 h, lower concentrations of Phytoplex promoted cell viability, while higher concentrations inhibited cell viability
(P < 0.05). In addition, the inhibitory effect of Phytoplex continuously increased from 24 to 72 h of incubation
peroid at high concentrations of Phytoplex. Moreover, Phytoplex inhibited HeLa cell proliferation with an IC50
value of 1,972.43, 1,230.10 and 1,317.67 μg/ml at 24, 48 and 72 h, respectively. The activity of caspase-3 of
HeLa cells treated with high concentration of Phytoplex seemed to be higher than of negative control (0 μg/ml).
Activity of Ki-67 was higher in lower concentrations of Phytoplex, than that in higher concentrations of
Phytoplex. Therefore, HeLa cell growth inhibition was dose-and time-dependent. The study suggested that high
concentrations (2,000 and 5,000 μg/ml) of Phytoplex exerted inhibitory effect in HeLa cell growth by inducing
apoptosis via activation of caspase-3.
Treatment of transitional cell carcinoma of urinary bladder using meloxicam in a dog: a case report
(2018) Kanokwan Suwankanit; Sukanya Manee-in; กนกวรรณ สุวรรณกนิษฐ์; สุกัญญา มณีอินทร์; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health
A 12-year-old spayed female poodle dog had clinical sign of stranguria. The dog was definitive diagnosed
by radiography, ultrasonography and histopathology that it was invasive transitional cell carcinoma at the neck
of urinary bladder. The dog was treated with meloxicam and black sesamin. The side effects of treatment,
blood profiles and urinalysis were investigated once a month and urinary bladder ultrasonographic examination
was performed every two months for disease progression monitoring. The dog had the stable size of mass. The
side effects of meloxicam were not observed during treatment.
Title
JAAS Vol. 11 No. 2
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์