Journal Issue: JAAS Vol. 10 No. 2
1
Issued Date
2560
Resource Type
Language
tha
eng
eng
File Type
application/pdf
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหิดล
Journal Volume
JAAS Volume 10
(2560)
JAAS Volume 11
(2561)
Articles
Effects of the three flavonoids; kaempferol, quercetin, and myricetin on Baby hamster kidney (BHK-21) cells and Human hepatocellular carcinoma cell (HepG2) cells proliferations and total Erk1/2 protein expression
(2017) Sookruetai Boonmasawai; Arpron Leesombun; Kridsada Chaichoun; Jarupha Taowan; Ladawan Sariya; Orathai Thongjuy; Mahidol University. Faculty of Veterinary Science
Kaempferol, quercetin, and myricetin were regarded as the potential therapeutic flavonoids in several
types of cancers. And the cancer cell proliferations that were directly induced by MAPK/Erk signaling pathway
had been the important target of cancer treatment. Thus, this study was investigated the anti-proliferative
effects of these three flavonoids together with total Erk1/2 protein expressions on Human hepatocellular
carcinoma cell (HepG2) and Baby hamster kidney cells (BHK-2). The data showed the cytotoxic effects of
100 μM kaempferol, 5 μM myricetin and 50 μM quercetin on BHK-21 cells at 24h. After same incubation time,
kaempferol (5 μM), myricetin (1 μM) and quercetin (1 μM) could significantly inhibit HepG2 cell proliferations.
By western blot analysis, kaempferol and myricetin could not affect Total Erk1/2 proteins expression. But
quercetin obviously suppressed total Erk1/2 protein expression in HepG2 cells at 24h. In conclusion, all three
flavonoids had significant inhibitory effects on Hepatocellular carcinoma cells without cytotoxic effects on
normal fibroblast. And inhibitory effects of quercetin in cancer cell proliferation related to total Erk1/2
protein reduction.
Stress cortisol and muscle stiffness in horses used for equine-assisted therapy
(2017) Nuttawut Nuchprayoon; Nlin Arya; Pattama Ritruechai; Mahidol University. Faculty of Veterinary Science
Equine-assisted therapy uses horseback riding to improve the patient's health and wellbeing. However,
the possible stress and damage of this therapy on the back horse muscle (longissimus dorsi) are understudied.
We studied the stress cortisol and muscle stiffness of two ponies and a horse used for equine-assisted therapy on
a child with autism. Salivary cortisol levels and heart rate were used as indicators of physiologic stress and a
muscle indenter was employed to estimate the back muscle stiffness. Saliva samples were collected before and
after equine-assisted therapy and then were analyzed by direct enzyme immunoassay technique. Heart rate
was measured by a Heart Rate Monitor at rest, during therapy at 5 minutes intervals for 30 minutes, and after
therapy at 5 minutes intervals for 20 minutes. The muscle stiffness and muscle tone along both sides of the
segments T14, T16, T18, and L2 of the longissimus dorsi muscle were obtained before and after equine-assisted
therapy. Both salivary cortisol and heart rate did not have significant differences as a consequence of the
therapy. Similarly, although a horse tended to have more muscle stiffness after the treatment, overall the
therapy had little effect or none on the horseback. The equine-assisted therapy does not seem to create a
negative impact on horses. However, further studies with a greater sample size in children and horses should
be carried out to confirm whether equine-assisted therapy is completely safe for horses' health.
Effects of adding melatonin on the quality of frozen-thawed boar semen
(2017) Natcha Thongrueang; Nutchanat Chaibangyang; Panida Chanapiwat; Kampon Kaeoket; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Health
The aim of this study was to study the effects of adding melatonin at different concentrations on the quality
of cryopreserved boar semen. Semen samples (n = 6) were collected by hand-glove technique. Semen samples
were diluted with Modena? and divided into 6 groups. According to the concentrations of melatonin at 0
(control, group A), 0.1 (group B), 0.5 (group C), 1.0 (group D), 1.5 (group E) and 2.0 mM (group F) to the lactose-egg
yolk extenders used to freeze boar semen with traditional method. Progressive motility, viability and acrosome
integrity were evaluated both before and after cryopreservation. The results showed that here was no significant
difference in percentage of progressive motility among groups. However, a higher percentage of progressive
motility was found in group D (39.17%). A higher percentage of sperm viability and acrosome integrity were
found in group F (28.33%) and group F (34.50%), respectively. In conclusion, the present results suggest that
adding melatonin between 0.1 and 1 mM during freezing yield a superior post-thawed semen qualities than
freezing without melatonin.
Title
JAAS Vol. 10 No. 2
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์