Publication: Effects of the three flavonoids; kaempferol, quercetin, and myricetin on Baby hamster kidney (BHK-21) cells and Human hepatocellular carcinoma cell (HepG2) cells proliferations and total Erk1/2 protein expression
2
Issued Date
2017
Resource Type
Language
eng
ISSN
1906-2257
Rights
Mahidol University
Rights Holder(s)
Faculty of Veterinary Science Mahidol University
Bibliographic Citation
Journal of Applied Animal Science. Vol.10, No.2 (2017), 23-34
Suggested Citation
Sookruetai Boonmasawai, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Ladawan Sariya, Orathai Thongjuy Effects of the three flavonoids; kaempferol, quercetin, and myricetin on Baby hamster kidney (BHK-21) cells and Human hepatocellular carcinoma cell (HepG2) cells proliferations and total Erk1/2 protein expression. Journal of Applied Animal Science. Vol.10, No.2 (2017), 23-34. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15258
Research Projects
Organizational Units
Authors
Journal Issue
JAAS Vol. 10 No. 2
(2560)
Thesis
Title
Effects of the three flavonoids; kaempferol, quercetin, and myricetin on Baby hamster kidney (BHK-21) cells and Human hepatocellular carcinoma cell (HepG2) cells proliferations and total Erk1/2 protein expression
Alternative Title(s)
ประสิทธิภาพของสารกลุ่มฟลาโวนอยด์สามชนิด ได้แก่ kaempferol quercetin และ myricetin ที่มีต่อการเพิ่มจำนวนของเซลล์ (proliferation) และการแสดงออกของโปรตีน total Erk1/2 ในเซลล์ Baby hamster kidney (BHK-21) และ Human hepatocellular carcinoma cell (HepG2)
Other Contributor(s)
Abstract
Kaempferol, quercetin, and myricetin were regarded as the potential therapeutic flavonoids in several
types of cancers. And the cancer cell proliferations that were directly induced by MAPK/Erk signaling pathway
had been the important target of cancer treatment. Thus, this study was investigated the anti-proliferative
effects of these three flavonoids together with total Erk1/2 protein expressions on Human hepatocellular
carcinoma cell (HepG2) and Baby hamster kidney cells (BHK-2). The data showed the cytotoxic effects of
100 μM kaempferol, 5 μM myricetin and 50 μM quercetin on BHK-21 cells at 24h. After same incubation time,
kaempferol (5 μM), myricetin (1 μM) and quercetin (1 μM) could significantly inhibit HepG2 cell proliferations.
By western blot analysis, kaempferol and myricetin could not affect Total Erk1/2 proteins expression. But
quercetin obviously suppressed total Erk1/2 protein expression in HepG2 cells at 24h. In conclusion, all three
flavonoids had significant inhibitory effects on Hepatocellular carcinoma cells without cytotoxic effects on
normal fibroblast. And inhibitory effects of quercetin in cancer cell proliferation related to total Erk1/2
protein reduction.