Publication: Pharmacological activity of Kaempferia parviflora extract against human bile duct cancer cell lines
Issued Date
2009-01-01
Resource Type
ISSN
2476762X
15137368
15137368
Other identifier(s)
2-s2.0-77951140167
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.10, No.4 (2009), 695-698
Suggested Citation
Vijittra Leardkamolkarn, Sunida Tiamyuyen, Bung orn Sripanidkulchai Pharmacological activity of Kaempferia parviflora extract against human bile duct cancer cell lines. Asian Pacific Journal of Cancer Prevention. Vol.10, No.4 (2009), 695-698. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27313
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Pharmacological activity of Kaempferia parviflora extract against human bile duct cancer cell lines
Other Contributor(s)
Abstract
A crude ethanol extract of Kaemperia parviflora Wall. Ex Baker and a purified compound, 5,7,4-trimethoxyflavone (KP.8.10), were evaluated for pharmacological effects on human cholangiocarcinoma cell lines (HuCCA-1 and RMCCA-1). The cells were incubated with various concentrations of extract for various time periods and metabolic activity (MTT assay) was assessed for cell viability. The results showed a dose-dependent effect of both crude ethanol extract and the pure compound. CC50s for the crude extract on HuCCA-1 and RMCCA-1 cells were 46.1μg/ml and 62.0μg/ml, respectively. Values for the pure compound could not be determined because of solubility problems. Interestingly, K. parviflora ethanol extract and KP.8.10 at low concentrations (10-20μg/ml and 2.5-5 μg/ml, respectively) markedly reduced rhHGF-induced invasion by HuCCA-1 and RMCCA-1 cells across matrix-coated transwell plates. Higher concentrations of K. parviflora ethanol extract (60 and 80μg/ml) and KP.8.10 (20 μg /ml) dramatically changed the cellular morphology and caused death in both cell types. KP.8.10 further exhibited progressive action via caspase-3 mitochondrial enzyme activation, enhancing cellular toxicity in a time-dose dependent fashion. Therefore, 5,7,4-trimethoxyflavone appeared to be a bioactive component of K. parviflora extract capable of exerting anti-cancer action. The results suggested a benefit of this edible plant in prevention and treatment of cholangiocarcinoma.