Publication: The hdhA gene encodes a haloacid dehalogenase that is regulated by the Lysr-type regulator, HdhR, in sinorhizobium meliloti
Issued Date
2013-06-01
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ISSN
15590305
10736085
10736085
Other identifier(s)
2-s2.0-84882939610
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular Biotechnology. Vol.54, No.2 (2013), 148-157
Suggested Citation
Ratiboot Sallabhan, Jarunee Kerdwong, James M. Dubbs, Kumpanart Somsongkul, Wirongrong Whangsuk, Phairin Piewtongon, Skorn Mongkolsuk, Suvit Loprasert The hdhA gene encodes a haloacid dehalogenase that is regulated by the Lysr-type regulator, HdhR, in sinorhizobium meliloti. Molecular Biotechnology. Vol.54, No.2 (2013), 148-157. doi:10.1007/s12033-012-9556-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31305
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Title
The hdhA gene encodes a haloacid dehalogenase that is regulated by the Lysr-type regulator, HdhR, in sinorhizobium meliloti
Abstract
The plasmid pSymA, in the nitrogen-fixing soil bacterium, Sinorhizobium meliloti, carries a 750-bp ORF (SMa1978) designated, hdhA, which encodes a novel dehalogenase that can detoxify haloacid compounds, showing a preference for haloacetic acids. Purified His-tagged HdhA demonstrated the apparent ability to dehalogenate chloroacetic acid and trifluoroacetic acid. In addition, upstream of hdhA, a gene encoding a lysR-type transcription regulator denoted, hdhR (SMa1979), has been identified to be a transcriptional repressor of hdhA expression. In an hdhR knockout mutant, hdhA promoter activity was markedly increased. Purified 32-kDa His-tagged HdhR repressed expression of hdhA by specifically binding to the promoter region of hdhA, as demonstrated by gel mobility shift assay and DNase I foot printing experiments. Moreover, the pesticide, pentachlorophenol, was also found to induce hdhA expression via HdhR. Site-directed mutants, in which the Cys residues at positions 160 and 192 in HdhR were changed to Ser, were constructed. C160S and C192S single mutants showed diminished HdhR-mediated repression of hdhA expression, while a C160S:C192S double mutant could no longer repress expression of hdhA. © 2012 Springer Science+Business Media, LLC.