Publication: Hydrogen peroxide toxicity induces ras signaling in human neuroblastoma SH-SY5Y cultured cells
Accepted Date
2010-08-18
Issued Date
2010
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Language
eng
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Mahidol University
Bibliographic Citation
Journal of Biomedicine and Biotechnology. 2010, ID 803815
Suggested Citation
Jirapa Chetsawang, Piyarat Govitrapong, Banthit Chetsawang Hydrogen peroxide toxicity induces ras signaling in human neuroblastoma SH-SY5Y cultured cells. Journal of Biomedicine and Biotechnology. 2010, ID 803815. doi:10.1155/2010/803815 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/1830
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Title
Hydrogen peroxide toxicity induces ras signaling in human neuroblastoma SH-SY5Y cultured cells
Abstract
It has been reported that overproduction of reactive oxygen species occurs after brain injury and mediates neuronal cells
degeneration. In the present study, we examined the role of Ras signaling on hydrogen peroxide-induced neuronal cells
degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y
cultured cells. An inhibitor of the enzyme that catalyzes the farnesylation of Ras proteins, FTI-277, and a competitive inhibitor
of GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y
cultured cells. The results of this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen peroxideinduced
toxicity in neuronal cells.