Publication: Enhanced production of bryonolic acid in Trichosanthes cucumerina L. (thai cultivar) cell cultures by elicitors and their biological activities
Issued Date
2020-06-01
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ISSN
22237747
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2-s2.0-85085997943
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Mahidol University
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SCOPUS
Bibliographic Citation
Plants. Vol.9, No.6 (2020), 1-12
Suggested Citation
Pornpatsorn Lertphadungkit, Jiraphong Suksiriworapong, Veena Satitpatipan, Supaart Sirikantaramas, Amaraporn Wongrakpanich, Somnuk Bunsupa Enhanced production of bryonolic acid in Trichosanthes cucumerina L. (thai cultivar) cell cultures by elicitors and their biological activities. Plants. Vol.9, No.6 (2020), 1-12. doi:10.3390/plants9060709 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57599
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Title
Enhanced production of bryonolic acid in Trichosanthes cucumerina L. (thai cultivar) cell cultures by elicitors and their biological activities
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Abstract
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Bryonolic acid is a triterpenoid compound found in cucurbitaceous roots. Due to its biological activities, this compound gets more attention to improve production. Herein, we carried out efficient ways with high bryonolic acid productions from Trichosanthes cucumerina L., a Thai medicinal plant utilizing plant cell cultures. The results showed that calli (24.65 ± 1.97 mg/g dry weight) and cell suspensions (15.69 ± 0.78 mg/g dry weight) exhibited the highest bryonolic acid productions compared with natural roots (approximately 2 mg/g dry weight). In the presence of three elicitors (methyl jasmonate, yeast extract, and chitosan), cell suspensions treated with 1 mg/mL of chitosan for eight days led to higher bryonolic acid contents (23.56 ± 1.68 mg/g dry weight). Interestingly, cell culture and root extracts with high bryonolic acid contents resulted in significantly higher percent cell viabilities than those observed under control (1% v/v DMSO) treatment in Saos-2 and MCF-7 cells. The present study indicated that T. cucumerina L. cell cultures are alternative and efficient to produce the biologically important secondary metabolite.