Publication: Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis
Issued Date
2007-01-01
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ISSN
1556679X
15566811
15566811
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2-s2.0-34247876275
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical and Vaccine Immunology. Vol.14, No.1 (2007), 110-113
Suggested Citation
Narisara Chantratita, Vanaporn Wuthiekanun, Aunchalee Thanwisai, Direk Limmathurotsakul, Allen C. Cheng, Wirongrong Chierakul, Nicholas P.J. Day, Sharon J. Peacock Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis. Clinical and Vaccine Immunology. Vol.14, No.1 (2007), 110-113. doi:10.1128/CVI.00289-06 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24294
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Title
Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis
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Abstract
Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%). Copyright © 2007, American Society for Microbiology. All Rights Reserved.