Publication: The urinary cotinine and serum 25 hydroxyvitamin D levels in male smokers
Issued Date
2012-12-01
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ISSN
01252208
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2-s2.0-84871887064
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.95, No.SUPPL 6 (2012)
Suggested Citation
Thanawan Buacharoen, Pornpimol Kongtip, Prapin Tharpoophasiam, Suttinun Chantanakul, Dusit Sujirarat The urinary cotinine and serum 25 hydroxyvitamin D levels in male smokers. Journal of the Medical Association of Thailand. Vol.95, No.SUPPL 6 (2012). Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14429
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Title
The urinary cotinine and serum 25 hydroxyvitamin D levels in male smokers
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Abstract
Objective: To assess urinary cotinine and the effects of smoking on 25 (OH) D levels in 67 male smokers. Material and Method: Urine and blood specimens were analyzed for cotinine and serum 25 hydroxyvitamin D (25 (OH) D) concentrations by high performance liquid chromatography (HPLC) and chemiluminescent immunoassay, respectively. The accuracy, precision and detection limit of the HPLC method were also tested. Results: The detection limit of urinary cotinine was 0.02 μg/ml. The recoveries of cotinine concentrations of 0.15-2.0 μg/ml were greater than 95%. Only 23.9% of smokers had sufficient levels of serum 25 (OH) D at least 30 ng/ml. The smokers were divided into dairy co-operative smokers and other smokers. The average urinary cotinine concentrations of 1,421.42 and 1,866.52 μg/g creatinine were not significant different in dairy co-operative smokers and other smokers whereas the average 25 (OH) D of 29.09 and 22.65 ng/ml, respectively, were significantly different at p-value of < 0.001. Conclusion: The 42.86% and 10.26% of the diary co-operative smokers and other smokers had sufficient serum 25 (OH) D levels to prevent osteoporosis.