Publication: The Effect of Temperature on the Stability of In-Use Insulin Pens
Issued Date
2021-09-01
Resource Type
ISSN
14393646
09477349
09477349
Other identifier(s)
2-s2.0-85114650757
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Experimental and Clinical Endocrinology and Diabetes. Vol.129, No.9 (2021), 683-688
Suggested Citation
Tanawan Kongmalai, Lukana Preechasuk, Sarawut Junnu, Siriphan Manocheewa, Chatchawan Srisawat, Apiradee Sriwijitkamol The Effect of Temperature on the Stability of In-Use Insulin Pens. Experimental and Clinical Endocrinology and Diabetes. Vol.129, No.9 (2021), 683-688. doi:10.1055/a-1010-5466 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76044
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The Effect of Temperature on the Stability of In-Use Insulin Pens
Other Contributor(s)
Abstract
Background Improper storage of insulin could decrease its potency. Manufacturers recommend that in-use insulin pens should be kept at between 25-30°C, but room temperature in tropical countries often exceeds this range. This study investigates the effect of temperature on the stability of basal insulin in cartridges 28 days after opening. Methods Four different basal insulins were evaluated. Five opened pens of each insulin type were included for each of three storage conditions and 5 unopened insulin pens of each type were stored in the refrigerator as a control. The opened pens were stored for 28 days in either a refrigerator (2-8 °C), at room temperature, or in an incubator (37 °C). Each day insulin pens were mixed 20 times and 2 units were discarded to mimic daily usage. Insulin quantity was evaluated using an ultra-high-performance liquid chromatography assay. Results The average room temperature during the study period was 29.7 °C. After 28 days, the percentage amount of insulin stored at refrigerator, room temperature or incubator, compared with control was 99.0, 99.7, 101.1% for long-acting insulin; 97.4, 97.2, 99.0% for NPH-1; 101.4, 101.5, 100.7% for NPH-2; and 98.7, 97.8, 98.5% for NPH-3. There were no statistically significant differences. However, we observed a trend toward different stability between clear insulin analog and turbid NPH insulin. Conclusions Temperature as high as 37°C and cyclic temperature,had no effect on the stability of in-use insulin pen.