Publication: Evaluation of the colchicine-like activity of Gloriosa superba-extracted fractions for mosquito (Diptera: Culicidae) cytogenetic study
Issued Date
2004-01-01
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ISSN
00222585
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2-s2.0-3342887227
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Medical Entomology. Vol.41, No.4 (2004), 672-676
Suggested Citation
W. Choochote, K. Rongsriyam, B. Pitasawat, A. Jitpakdi, E. Rattanachanpichai, A. Junkum, B. Tuetun, P. Chaiwong Evaluation of the colchicine-like activity of Gloriosa superba-extracted fractions for mosquito (Diptera: Culicidae) cytogenetic study. Journal of Medical Entomology. Vol.41, No.4 (2004), 672-676. doi:10.1603/0022-2585-41.4.672 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21099
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Title
Evaluation of the colchicine-like activity of Gloriosa superba-extracted fractions for mosquito (Diptera: Culicidae) cytogenetic study
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Abstract
Four fractions of Gloriosa superba L., i.e., hexane fraction, dichloromethane fraction 1, dichloromethane fraction 2, and methanol fraction, were investigated for colchicine-like activity using a mosquito cytogenetic assay. The results revealed that the latter three fractions yielded promisingly high colchicine-like activity, whereas the hexane fraction yielded very low activity compared with 1% colchicine in a 0.85% sodium chloride solution. The metaphase rates and average number of metaphase chromosomes per positive brain ganglion (range) of Aedes aegypti L. larvae after incubation with 0.25-2% solutions of dichloromethane fraction 1, dichloromethane fraction 2, 0.5-2% solutions of methanol fraction, and 1% colchicine solution were 90-100% and 7 (2-19) to 22 (7-47); 90-100% and 4 (1-11) to 30 (4-73); 95-100% and 11 (1-28) to 17 (2-62); and 100% and 6 (2-11), respectively. The temperature stability tests of the three promising fractions were performed by heating 0.5% working solution at 121°C for 15 min and preparing 0.5% working solution from stock frozen at -20°C for 10 mo. These fractions also yielded satisfactory outcomes of metaphase rates and an average number of metaphase chromosomes per positive brain ganglia compared with 1% colchicine solution.