Publication: β-Thalassaemia/haemoglobin E tissue ferritins - I: Purification and partial characterization of liver and spleen ferritins
Issued Date
1990-09-01
Resource Type
ISSN
15728773
09335854
09335854
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2-s2.0-0025685052
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Mahidol University
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SCOPUS
Bibliographic Citation
Biology of Metals. Vol.3, No.3-4 (1990), 222-226
Suggested Citation
Kim Chi Tran, John Webb, David J. Macey, Pensri Pootrakul, Pornpan Yansukon β-Thalassaemia/haemoglobin E tissue ferritins - I: Purification and partial characterization of liver and spleen ferritins. Biology of Metals. Vol.3, No.3-4 (1990), 222-226. doi:10.1007/BF01140583 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15895
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Title
β-Thalassaemia/haemoglobin E tissue ferritins - I: Purification and partial characterization of liver and spleen ferritins
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Abstract
Ferritins from liver and spleen of both β-thalassaemia/haemoglobin E (HbE) and non-thalassaemic patients were purified by heating a methanol-treated homogenate, followed by molecular exclusion chromatography. The concentrations of ferritins in the β-thalassaemia/HbE liver and spleen were calculated as 3.8 and 2.0 mg/g wet tissue. The β-thalassaemia/HbE ferritin iron/protein ratios were higher than those of normal ferritins. On PAGE, all ferritins gave a single major monomeric band with only very small differences in their mobility. Ferritins from thalassaemic patients also possessed bands corresponding to oligomers. On SDS/PAGE, all ferritins were resolved into two major subunits: H and L with L subunit predominating. While the isoferritin profiles of ferritins from β-thalassaemia/HbE liver and spleen were similar to each other and to those of normal liver and spleen, some extra bands were present in the acidic region. The microstructure of these pathological ferritins appears to result, to a large degree, from the particular nature and amount of iron loading present. © 1990 Springer-Verlag.