Publication: A sensitive non-aqueous capillary electrophoresis-mass spectrometric method for multiresidue analyses of β-agonists in pork
Issued Date
2010-06-01
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ISSN
10990801
02693879
02693879
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2-s2.0-77952775234
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Mahidol University
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SCOPUS
Bibliographic Citation
Biomedical Chromatography. Vol.24, No.6 (2010), 588-599
Suggested Citation
Oraphan Anurukvorakun, Wolfgang Buchberger, Markus Himmelsbach, Christian W. Klampel, Leena Suntornsuk A sensitive non-aqueous capillary electrophoresis-mass spectrometric method for multiresidue analyses of β-agonists in pork. Biomedical Chromatography. Vol.24, No.6 (2010), 588-599. doi:10.1002/bmc.1331 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28695
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Title
A sensitive non-aqueous capillary electrophoresis-mass spectrometric method for multiresidue analyses of β-agonists in pork
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Abstract
Non-aqueous capillary electrophoresis-mass spectrometry (NACE-MS) was developed for trace analyses of β-agonists (i.e. clenbuterol, salbutamol and terbutaline) in pork. The NACE was in 18 mM ammonium acetate in methanol-acetonitrile-glacial acetic acid (66 : 33 : 1, v/v/v) using a voltage of 28 kV. The hyphenation of CE with a time-of-flight MS was performed by electrospray ionization interface employing 5 mM ammonium acetate in methanol-water (80 : 20, v/v) as the sheath liquid at a flow rate of 2 μL/min. Method sensitivity was enhanced by a co-injection technique (combination of hydrodynamic and electrokinetic injection) using a pressure of 50 mbar and a voltage of 10 kV for 12 s. The method was validated in comparison with HPLC-MS-MS. The NACE-MS procedure provided excellent detection limits of 0.3 ppb for all analytes. Method linearity was good (r2 > 0.999, in a range of 0.8-1000 ppb for all analytes). Precision showed %RSDs of <17.7%. Sample pre-treatment was carried out by solid-phase extraction using mixed mode reversed phase/cation exchange cartridges yielding recoveries between 69 and 80%. The NACE-MS could be successfully used for the analysis of β-agonists in pork samples and results showed no statistical differences from the values reported by the Ministry of Public Health, Thailand using HPLCMS-MS method. Copyright © 2009 John Wiley & Sons, Ltd.