Publication: Electron microscopy study of the mode of growth of Pseudomonas pseudomallei in vitro and in vivo
Issued Date
1995-12-01
Resource Type
ISSN
00225304
Other identifier(s)
2-s2.0-0029417175
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Tropical Medicine and Hygiene. Vol.98, No.6 (1995), 379-391
Suggested Citation
M. Vorachit, K. Lam, P. Jayanetra, J. W. Costerton Electron microscopy study of the mode of growth of Pseudomonas pseudomallei in vitro and in vivo. Journal of Tropical Medicine and Hygiene. Vol.98, No.6 (1995), 379-391. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17287
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Title
Electron microscopy study of the mode of growth of Pseudomonas pseudomallei in vitro and in vivo
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Abstract
The mode of growth of Pseudomonas pseudomallei in culture media and in the lung tissue of infected humans and animals was studied using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). In culture media, P. pseudomallei cells were seen to be entrapped in microcolonies within large amounts of intercellular fibrous material. The lung tissue of infected humans and animals showed that bacterial cells growing in lung tissue were surrounded by radially arranged fibres that constitute a very well defined glycocalyx structure. In the infected areas of the animal lung tissue, bacterial cell could be seen to have formed glycocalyx enclosed microcolonies that displaced host cell components, e.g. the nucleus of a phagocyte. The presence of bacteria in unusual locations indicated that effective phagocytosis was not occurring. The demonstration that cells of P. pseudomallei produce exopolysaccharide glycocalyces and form glycocalyx enclosed microcolonies in laboratory media and in lung tissue of infected humans and animals and the presence of bacteria in unusual locations contribute to a new understanding of the mechanism whereby this organism can cause persistent chronic infections.