Publication: A new acidic protease in human seminal plasma
Issued Date
1974-07-10
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-0016231341
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.59, No.1 (1974), 44-50
Suggested Citation
Pintip Ruenwongsa, Montri Chulavatnatol A new acidic protease in human seminal plasma. Biochemical and Biophysical Research Communications. Vol.59, No.1 (1974), 44-50. doi:10.1016/S0006-291X(74)80171-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/10603
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Title
A new acidic protease in human seminal plasma
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Abstract
A new acidic protease with pH optimum of 3.0 was partially purified from human seminal plasma. It can hydrolyze hemoglobin and N, N-dimethyl casein. It was stable in acidic but labile in neutral and alkaline solutions. Its molecular weight was determined by gel filtration to be 40,000. Its chromatographic behavior on carboxy methyl Sephadex column was quite different from the pH-8.0 protease of human seminal plasma. It was present in seminal plasma of normal and vasectomized individuals. Its activity was not affected by cysteine, metal cations or ethylene diamine tetraacetic acid. © 1974 Academic Press, Inc.