Publication: Application of surface plasmon resonance biosensor for the detection of Candida albicans
Issued Date
2016-02-01
Resource Type
ISSN
13474065
00214922
00214922
Other identifier(s)
2-s2.0-84956625420
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Mahidol University
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SCOPUS
Bibliographic Citation
Japanese Journal of Applied Physics. Vol.55, No.2 (2016)
Suggested Citation
Sirasa Yodmongkol, Sroisiri Thaweboon, Boonyanit Thaweboon, Chokchai Puttharugsa, Boonsong Sutapun, Ratthasart Amarit, Armote Somboonkaew, Toemsak Srikhirin Application of surface plasmon resonance biosensor for the detection of Candida albicans. Japanese Journal of Applied Physics. Vol.55, No.2 (2016). doi:10.7567/JJAP.55.02BE03 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40635
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Title
Application of surface plasmon resonance biosensor for the detection of Candida albicans
Abstract
© 2016 The Japan Society of Applied Physics. In this study, surface plasmon resonance imaging (SPR imaging) was developed for the detection of Candida albicans which is a causal agent of oral infection. The detection was based on the sandwich assay. The capture antibody was covalently immobilized on the mixed self assemble monolayers (SAMs). The ratio of mixed SAMs between 11-mercaptoundecanoic acid and 3-mercaptopropanol was varied to find the optimal ratio for use as a sensor surface. The results showed that the suitable surface for C. albicans detection was SAM of carboxylic (mixed SAMs 1 : 0), even though mixed SAMs 1 : 40 had a high detection signal in comparison to mixed SAMs 1 : 0, but the non-specific signal was higher. The detection limit was 107cells/ml for direct detection, and was increased to 106cells/ml with sandwich antibody. The use of polyclonal C. albicans antibody as capture and sandwich antibody showed good selectivity against the relevant oral bacteria including Escherichia coli, Streptococcus mutan, Staphylococcus aureus, β-streptococci, and Lactobacillus casei. SPR platform in this study could detect C. albicans from the mixed microbial suspension without requirement of skillful technician. This SPR imaging biosensor could be applied for Candida identification after cultivation.