Scopus 2016-2017
Permanent URI for this collectionhttps://repository.li.mahidol.ac.th/handle/123456789/40568
Browse
Recent Submissions
Publication Metadata only Salivary oxidative stress biomarkers in chronic periodontitis and acute coronary syndrome(2017-09-01) Thuy T. Nguyen; Lan Q. Ngo; Ananya Promsudthi; Rudee Surarit; Mahidol University; University of Medicine and Pharmacy© 2016, Springer-Verlag Berlin Heidelberg. Objectives: The study aimed at assessing oxidative stress (OS) biomarker levels in the saliva of patients with chronic periodontitis (CP) and acute coronary syndrome (ACS) and establishing their correlation to periodontal parameters and markers for cardiovascular events. Materials and methods: The present study enrolled 24 patients with ACS and CP (the ACSCP group), 24 patients with ACS only (the ACS group), 24 patients with CP only (the CP group), and 24 healthy controls. Plaque index (PI), gingival index, bleeding on probing, probing pocket depth (PPD), and clinical attachment loss were recorded. Markers for cardiovascular events included serum high sensitivity C-reactive protein (hsCRP) and plasma fibrinogen. 8-Hydroxydeoxyguanosine (8-OHdG), protein carbonyl (PC), malondialdehyde (MDA), and total antioxidant capacity (TAOC) were used as OS biomarkers. Results: Salivary 8-OHdG, MDA, and PC levels were significantly higher in the ACSCP, ACS, and CP groups than in healthy controls (p < 0.05). There were significant correlations between salivary PC levels and PI or PPD (p < 0.05) as well as between salivary 8-OHdG levels and all periodontal parameters (p < 0.05). TAOC levels in saliva were correlated to both serum hsCRP and plasma fibrinogen (p < 0.05). Salivary MDA levels were correlated to all periodontal parameters and biomarkers for cardiovascular events (p < 0.05). Conclusions: Salivary OS biomarker levels were higher in diseased groups compared to control. They also correlated to clinical periodontal parameters and markers for cardiovascular events in ACS patients, with or without CP. Clinical relevance: Salivary OS biomarkers could potentially serve as diagnostic tools for cardiovascular and/or periodontal diseases.Publication Metadata only Centrality searching on signal transduction pathway for drug selection support system in targeted cancer therapy(2017-02-21) Massaya Samnienggam; Tanasanee Phienthrakul; Mahidol University© 2016 IEEE. Cancer is a leading cause of death in the world. Many researchers are still searching for cancer treatments as well. This paper proposes a technique to support the cancer treatments, call Targeted Cancer Therapy. This treatment affects specific to cancer cells to inhibit signal transduction processes because the signal transduction is the cause of the growth and division of cancer cells. Normally, this treatment will take drugs to the target gene that causes cancer. However, sometimes, there is no drug for the target gene. Signal transduction pathway can be used for analyzing the related drug. The drug searching in pathway is considered in this research. Graph traversal such as Depth First Search (DFS) and Breadth First Search (BFS) are used as the searching techniques in the pathway. Centrality measures are applied to graph traversal in order to increase performance of search results. Drug results are scored and measured. Experimental results show that the betweenness centrality on depth first search yields a set of suitable drugs that is higher than those of degree and closeness centrality.Publication Metadata only One-step Preparation of Carbon-based Solid Acid Catalyst from Water Hyacinth Leaves for Esterification of Oleic Acid and Dehydration of Xylose(2017-12-14) Jutitorn Laohapornchaiphan; Christopher B. Smith; Siwaporn Meejoo Smith; Mahidol University© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Carbon-based solid acid catalysts were successfully obtained via one-step hydrothermal carbonization (HTC) of water hyacinth (WH) in the presence of p-toluenesulfonic acid (PTSA). Increasing the HTC temperature from 180 to 240 °C resulted in carbonaceous materials with increased sulfur content and less adsorbed water. The material obtained at 220 °C (WH-PTSA-220) contains the highest amount of acid sites and promotes the highest initial rate of two transformations, that is, methanolysis of oleic acid and dehydration of xylose to furfural. While all PSTA-treated WH catalysts gave comparable fatty acid conversions (≈97 %) and furfural yields (≈60 %) after prolonged reaction times, the WH-PTSA-240 system bearing a relatively low acid density maintains the most favorable reusability profile. Higher HTC temperatures (220–240 °C) improved the catalyst reusability profiles due to graphitization and hydrophobicity of the carbon surface. The catalyst systems derived herein from biomass may have potential applications in biorefining platforms, utilizing the conversion of waste biomass to chemicals.Publication Metadata only HbE/β-Thalassemia and Oxidative Stress: The Key to Pathophysiological Mechanisms and Novel Therapeutics(2017-05-10) Rhoda Elison Hirsch; Nathawut Sibmooh; Suthat Fucharoen; Joel M. Friedman; Albert Einstein College of Medicine of Yeshiva University; Mahidol University© Copyright 2017, Mary Ann Liebert, Inc. Significance: Oxidative stress and generation of free radicals are fundamental in initiating pathophysiological mechanisms leading to an inflammatory cascade resulting in high rates of morbidity and death from many inherited point mutation-derived hemoglobinopathies. Hemoglobin (Hb)E is the most common point mutation worldwide. The βE-globin gene is found in greatest frequency in Southeast Asia, including Thailand, Malaysia, Indonesia, Vietnam, Cambodia, and Laos. With the wave of worldwide migration, it is entering the gene pool of diverse populations with greater consequences than expected. Critical Issues: While HbE by itself presents as a mild anemia and a single gene for β-thalassemia is not serious, it remains unexplained why HbE/β-thalassemia (HbE/β-thal) is a grave disease with high morbidity and mortality. Patients often exhibit defective physical development, severe chronic anemia, and often die of cardiovascular disease and severe infections. Recent Advances: This article presents an overview of HbE/β-thal disease with an emphasis on new findings pointing to pathophysiological mechanisms derived from and initiated by the dysfunctional property of HbE as a reduced nitrite reductase concomitant with excess α-chains exacerbating unstable HbE, leading to a combination of nitric oxide imbalance, oxidative stress, and proinflammatory events. Future Directions: Additionally, we present new therapeutic strategies that are based on the emerging molecular-level understanding of the pathophysiology of this and other hemoglobinopathies. These strategies are designed to short-circuit the inflammatory cascade leading to devastating chronic morbidity and fatal consequences. Antioxid. Redox Signal. 26, 794-813.Publication Metadata only Micropropagation of Dendrobium cruentum Rchb. f., a rare Thai orchid species(2017-07-21) S. Sangdum; K. Thammasiri; N. Chuenboonngarm; K. Sopalun; Mahidol University; Kasetsart University© 2017, International Society for Horticultural Science. All rights reserved. Shoot tips of Dendrobium cruentum Rchb. f. were cultured on 1/2 Murashige and Skoog (MS) liquid medium supplemented with 1% (w/v) sucrose and 1.0 ppm NAA. The survival rate, PLB (protocorm-like body) formation, number of PLBs shoot-1 and relative growth rate were 69, 69%, 1 PLB shoot-1 and 139%, respectively. The highest production of shoots were observed on PLBs cultured on Vacin and Went (VW) agar medium supplemented with 1.5 ppm BA. This media gave the survival rate, shoot formation, number of shoots PLB-1, root formation and number of roots PLB-1 of 77, 75%, 34 shoots PLB-1, 63% and 27 roots PLB-1, respectively.Publication Metadata only Phytoremediation of petroleum-contaminated soil in association with soil bacteria(2017-01-01) Prayad Pokethitiyook; Mahidol University© Springer International Publishing AG 2017. Unprecedented progress in industrial activities over the last century has directly contributed to the discharge of huge amounts of petroleum hydrocarbons into the environment. It has been estimated that about 1.7–8.8 million metric tons of oil is released into the environment every year. More than 90% of this oil pollution is caused by accidents due to human errors and also deliberate disposal of the waste containing hydrocarbons. Generally, petroleum and its products get into the environment through natural seepages, transportation, accidental spills, deliberate disposal, offshore production, and breakage of pipelines. Presence of petroleum hydrocarbon compounds in the environment can affect both on human health and the environment. Therefore, their presence in nature is of great concern today, and they need to be cleaned from the environment in the best possible way. Many research works have been carried out to determine the eco-toxicity of these pollutants but biological method has been reported to be more suitable to determine the possible hazards of pollutants in soil on the ecological and environmental bases.Publication Metadata only Zoonotic potential of Enterocytozoon genotypes in humans and pigs in Thailand(2017-01-15) Rapeepun Prasertbun; Hirotake Mori; Ai rada Pintong; Suparut Sanyanusin; Supaluk Popruk; Chalit Komalamisra; Tanasak Changbunjong; Ruangrat Buddhirongawatr; Yaowalark Sukthana; Aongart Mahittikorn; Mahidol University© 2016 Enterocytozoon bieneusi is an opportunistic intestinal pathogen infecting humans and a variety of animals. Its mode of transmission and zoonotic potential are not completely understood. E. bieneusi has been frequently identified in pigs. The objective of our study was to investigate E. bieneusi in pigs and humans in Western and Central Thailand to determine its presence, genetic diversity, and zoonotic potential. A total of 277 human and 210 pig faecal samples were collected and analysed. E. bieneusi was found in 5.4% and 28.1% of human and pig samples, respectively, by nested PCR. Genotyping based on the internal transcribed spacer regions of the small subunit ribosomal RNA demonstrated three known genotypes (D, H, PigEb10) and eight novel genotypes (TMH1–8) in humans, and five known genotypes (D, EbpA, EbpC, H, O) and 11 novel genotypes (TMP1–11) in pigs. All known genotypes identified in humans and pigs had zoonotic potential. Further studies are needed to evaluate zoonotic risk of novel genotypes, as pigs may play an important role in the transmission of E. bieneusi.Publication Metadata only Comparison of friction forces between stainless orthodontic steel brackets and TiNi wires in wet and dry conditions(2017-03-01) Aphinan Phukaoluan; Anak Khantachawana; Pongpan Kaewtatip; Surachai Dechkunakorn; Niwat Anuwongnukroh; Peerapong Santiwong; Julathep Kajornchaiyakul; King Mongkut s University of Technology Thonburi; Mahidol University; Thailand National Metal and Materials Technology Center© 2016 CEO In sliding mechanics, frictional force is an important counter-balancing element to orthodontic tooth movement, which must be controlled in order to allow application of light continuous forces. The purpose of this study was to compare the frictional forces between a stainless steel bracket and five different wire alloys under dry and wet (artificial saliva) conditions. TiNi, TiNiCu, TiNiCo, commercial wires A and commercial wires B with equal dimensions of 0.016 × 0.022′′ were tested in this experiment. The stainless steel bracket was chosen with a slot dimension of 0.022′′. Micro-hardness of the wires was measured by the Vickers micro-hardness test. Surface topography of wires was measured by an optical microscope and quantified using surface roughness testing. Static and kinetic friction forces were measured using a custom-designed apparatus, with a 3-mm stretch of wire alloy at a crosshead speed of 1 mm/min. The static and dynamic frictions in the wet condition tended to decrease more slowly than those in the dry condition. Therefore, the friction of TiNiCu and commercial wires B would increase. Moreover, these results were associated with scarred surfaces, i.e. the increase in friction would result in a larger bracket microfracture. From the results, it is seen that copper addition resulted in an increase in friction under both wet and dry conditions. However, the friction in the wet condition was less than that in dry condition due to the lubricating effect of artificial saliva.Publication Metadata only Hydrothermal carbonization of oil palm pressed fiber: Effect of reaction parameters on product characteristics(2017-06-01) Kamonwat Nakason; Bunyarit Panyapinyopol; Vorapot Kanokkantapong; Nawin Viriya-Empikul; Wasawat Kraithong; Prasert Pavasant; Mahidol University; Center of Excellence on Environmental Health and Toxicology (EHT); Chulalongkorn University; King Mongkut s University of Technology Thonburi; Thailand National Science and Technology Development Agency; Vidyasirimedhi Institute of Science and TechnologyThis work focused on the hydrothermal carbonization (HTC) of oil palm pressed fiber for the production of valuable products, over the reaction temperature (140 to 200 °C), time (1 to 4 h), and biomass to water content (BTW) (1:5 to 1:15). Reaction temperature was a vital key parameter on fuel properties of hydrochar. The combustibility of hydrochar reflected by fuel ratio increased from 0.42 at 140 °C to 0.54 at 200 °C. O/C and H/C atomic ratio decreased from 0.56 and 1.54 to 0.37 and 1.28, respectively, which corresponded to an increase in the estimated HHV from 21.0 to 24.6 MJ/kg. Hydrochar yield decreased from 78.4 to 61.7%. These phenomena were associated with hydrolysis, dehydration, decarboxylation, aromatization, and recondensation reactions. The reaction time and BTW affected slightly the yield and fuel properties of hydrochar. The valuable dissolved organic chemical species in the obtained liquid fraction were furfuryl alcohol, furfural, hydroxymethylfurfural, acetic acid, propionic acid, lactic acid, and formic acid with the maximum yields of 6.95, 2.05, 0.64, 6.91, 4.75, 4.11 and 2.64 wt.% dry raw material, respectively.Publication Metadata only Phthalate exposure in Thai children and adolescents(2017-08-01) Nuttanun Sedtasiriphokin; Vichit Supornsilchai; Chutima Jantarat; Wichit Nosoongnoen; Chulalongkorn University; Walailak University; Mahidol University© 2017 Nuttanun Sedtasiriphokin, Vichit Supornsilchai, Chutima Jantarat, Wichit Nosoongnoen This Work is licensed under Creative Common License Background: Phthalates are found in products made of plastic. Because of concerns regarding the hazards of phthalate exposure, including endocrine disruption, many countries have regulations to restrict their use in products used by children. However, in Thailand, no such restrictions exist, and data relating to phthalate exposure are scarce. Objectives: To determine the level of exposure of Thai children and adolescents to phthalates, and study its associations with sociodemographic data and the exposure to potential sources of phthalates. Methods: Healthy children aged 2-18 y were enrolled into the present cross-sectional study between January 2016 and December 2016 inclusive. Their anthropometric indices and Tanner staging were determined. Urinary concentrations of the phthalate metabolites, monomethyl phthalate (MMP) and mono-n-butyl phthalate (MBP), were determined in spot samples by high-performance liquid chromatography to estimate the level of phthalate exposure. Associations between sociodemographic data, exposure to potential sources of phthalates, and phthalate metabolite concentrations were analyzed. Results: We included 103 boys and 118 girls with a mean age of 9.4 ± 3.64 (range 2.8-17.1) y and detected MMP in 28.5% and MBP in 88.6%. The geometric means (interquartile range) of urinary MMP and MBP were 3400 (2489, 4642) and 214.4 (164, 279) mg/g creatinine (Cr), respectively. Significant associations were found between exposure to floor cleaning products and Cr-adjusted urinary MMP level (P < 0.05), and paint and Cr-adjusted urinary MMP and MBP levels (P < 0.05). Prepuberty was significantly associated with urinary Cr-adjusted MMP level. Conclusion: Urinary phthalate metabolite levels were high in a proportion of Thai children and adolescents. Exposure to floor cleaning products and paint is associated with phthalate exposure, and advanced Tanner stage is negatively associated with urinary Cr-adjusted MBP.Publication Metadata only Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells(2017-12-01) Thannicha Sae-lao; Natthanej Luplertlop; Tavan Janvilisri; Rutaiwan Tohtong; David O. Bates; Kanokpan Wongprasert; Mahidol University; University of Nottingham© 2017 Elsevier GmbH Background Seaweeds have a long history of use in Asian countries as functional foods, medicinal herbs, and the treatment of cancer. Polysaccharides from various seaweeds have shown anti-tumor activity. Cholangiocarcinoma (CCA), often with metastatic disease, is highly prevalent in Thailand as a consequence of liver fluke infection. Recently, we extracted sulfated galactans (SG) from Gracilaria fisheri (G. fisheri), a south east Asian seaweed, and found it exhibited anti-proliferation effect on CCA cells. Purpose In the present study, we evaluated the anti-migration activity of SG on CCA cells and its underlined mechanism. Methods CCA cells were treated with SG alone or drugs targeting to epidermal growth factor (EGF) receptor (EGFR) or pretreated with SG prior to incubation with EGF. Anti-migration activity was determined using a scratch wound-healing assay and zymography. Immunofluorescence staining and western blotting were used to investigate EGFR signaling mediators. Results Under basal condition, SG reduced the migration rate of CCA, which was correlated with a decrease in the active-form of matrix metalloproteinases-9. SG decreased expression of phosphorylated focal adhesion kinase (FAK), but increased expression of E-cadherin to promote cells stasis. Moreover, phosphorylation of EGFR and extracellular signal-regulated kinases (ERK), known to stimulate growth of cancer cells, was blocked in a comparable way to EGFR inhibitors Cetuximab and Erlotinib. Pretreatment cells with SG attenuated EGF induced phosphorylation of EGFR, ERK and FAK. Conclusion This study reveals that SG from G. fisheri retards migration of CCA cells, and its mechanism of inhibition is mediated, to some extent, by inhibitory effects on MAPK/ERK signal transduction pathway. Our findings suggest that there may be a therapeutic potential of SG in CCA treatment.Publication Metadata only Detection and drug resistance profile of Escherichia coli from subclinical mastitis cows and water supply in dairy farms in Saraburi Province, Thailand(2017-01-01) Woranich Hinthong; Natapol Pumipuntu; Sirijan Santajit; Suphang Kulpeanprasit; Shutipen Buranasinsup; Nitat Sookrung; Wanpen Chaicumpa; Pisinee Aiumurai; Nitaya Indrawattana; HRH Princess Chulabhorn College of Medical Science; Mahidol University; Mahasarakham University© 2017 Hinthong et al. Subclinical mastitis is a persistent problem in dairy farms worldwide. Environmental Escherichia coli is the bacterium predominantly responsible for this condition. In Thailand, subclinical mastitis in dairy cows is usually treated with various antibiotics, which could lead to antibiotic resistance in bacteria. E. coli is also a reservoir of many antibiotic resistance genes, which can be conveyed to other bacteria. In this study, the presence of E. coli in milk and water samples was reported, among which enteropathogenic E. coli was predominant, followed by enteroaggregative E. coli and enterohemorrhagic E. coli, which was found only in milk samples. Twenty-one patterns of antibiotic resistance were identified in this study. Ampicillin- and carbenicillin- resistant E. coli was the most common among the bacterial isolates from water samples. Meanwhile, resistance to ampicillin, carbenicillin, and sulfamethoxazole-trimethoprim was the pattern found most commonly in the E. coli from milk samples. Notably, only the E. coli from water samples possessed ESBL phenotype and carried antibiotic resistance genes, blaTEMand blaCMY-2. This indicates that pathogenic E. coli in dairy farms is also exposed to antibiotics and could potentially transfer these genes to other pathogenic bacteria under certain conditions.Publication Metadata only DID: Auto censorship document(2017-10-19) Nichaboon Rattanabunsakul; Atcha Srisittichaikul; Areeya Sriprasert; Damras Wongsawang; Mahidol University© 2017 IEEE. Detection of Inappropriate Document (DID) is an automatic document censorship to censor for Thai inappropriate words and change the words to symbol star(*). DID program has been developed as an additional tool on Google Chrome and a website platform. The purpose of DID is to detect impolite words or contents on a document and social media. The program uses THSplitLib, PhpMyAdmin manages, HTML, CSS, Bootstrap, PHP, and Xampp for developing and managing process. Furthermore, inexact string matching is also implemented to detect impolite words which are intentionally misspell. From the experiments, the accuracy of DID in detection is more than 91 percents. However, there are some errors due to variety of word typing and meaning.Publication Metadata only P40: Gait alteration in patients with Idiopathic Normal Pressure Hydrocephalus after cerebral spinal fluid removal(2017-01-01) Sunee Bovonsunthonchai; Theerapol Witthiwej; Chanon Ngamsombat; Sith Sathornsumetee; Roongtiwa Vachalathiti; Mahidol UniversityPublication Metadata only Size-controlled preparation of gold nanoclusters on hydroxyapatite through trans-deposition method(2017-01-01) Setsiri Haesuwannakij; Thinnaphat Poonsawat; Medena Noikham; Ekasith Somsook; Yumi Yakiyama; Raghu Nath Dhital; Hidehiro Sakurai; Sokendai Graduate University for Advanced Studies; Mahidol University; Osaka UniversityCopyright © 2017 American Scientific Publishers All rights reserved. This paper describes a trans-deposition method for the size-controlled preparation of metal oxide-supported gold nanoclusters (AuNCs) varying from 1 to 8 nm in diameter. Colloidal AuNCs of various sizes were successfully deposited on hydroxyapatite (HAP), without undergoing a significant change in size from the initial polymer-supported AuNCs. The resulting AuNCs deposited on HAP (Au:HAP) were characterized by transmission electron microscope, powder X-ray diffraction, X-ray photoelectron spectroscope, inductively coupled plasma atomic emission spectroscopy, and X-ray absorption spectroscopy. The Au:HAP thus obtained catalyzed the aerobic oxidation of 1-indanol to give 1-indanone with excellent yield and recyclability.Publication Metadata only Four human Plasmodium species quantification using droplet digital PCR(2017-04-01) Suttipat Srisutham; Naowarat Saralamba; Benoit Malleret; Laurent Rénia; Arjen M. Dondorp; Mallika Imwong; Mahidol University; A-Star, Singapore Immunology Network; Yong Loo Lin School of Medicine; University of Oxford© 2017 Srisutham et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Droplet digital polymerase chain reaction (ddPCR) is a partial PCR based on water-oil emulsion droplet technology. It is a highly sensitive method for detecting and delineating minor alleles from complex backgrounds and provides absolute quantification of DNA targets. The ddPCR technology has been applied for detection of many pathogens. Here the sensitive assay utilizing ddPCR for detection and quantification of Plasmodium species was investigated. The assay was developed for two levels of detection, genus specific for all Plasmodium species and for specific Plasmodium species detection. The ddPCR assay was developed based on primers and probes specific to the Plasmodium genus 18S rRNA gene. Using ddPCR for ultra-sensitive P. falciparum assessment, the lower level of detection from concentrated DNA obtained from a high volume (1 mL) blood sample was 11 parasites/mL. For species identification, in particular for samples with mixed infections, a duplex reaction was developed for detection and quantification P. falciparum/ P. vivax and P. malariae/ P. ovale. Amplification of each Plasmodium species in the duplex reaction showed equal sensitivity to singleplex single species detection. The duplex ddPCR assay had higher sensitivity to identify minor species in 32 subpatent parasitaemia samples from Cambodia, and performed better than real-time PCR. The ddPCR assay shows high sensitivity to assess very low parasitaemia of all human Plasmodium species. This provides a useful research tool for studying the role of the asymptomatic parasite reservoir for transmission in regions aiming for malaria elimination.Publication Metadata only PENTOS: Penetration testing tool for Internet of Thing devices(2017-12-19) Vasaka Visoottiviseth; Phuripat Akarasiriwong; Siravitch Chaiyasart; Siravit Chotivatunyu; Mahidol University© 2017 IEEE. The Internet of Thing (IoT) technology has been growing rapidly with many implementations. However, because of its ability to perform tasks and handle the sensitive information and also the paucity of user security awareness, IoT devices contain many potential risks and are the new target of attacks. In this paper, we develop a penetration testing system for IoT devices called PENTOS in order to increase the user security awareness. The system comes with the GUI running on Kali Linux which is specifically designed for ethical hacking. PENTOS automatically gathers the information of the target IoT device through the wireless communication, which are WiFi and Bluetooth. The system allows users to perform various kinds of penetration testing on their IoT devices such as the password attack, web attack, and wireless attack in order to gain the privilege access by multiple algorithms. Moreover, the system also provides the basic security guidance according to the OWASP's Top 10 IoT Vulnerabilities to educate users and increase the security awareness. After the penetration testing, the system then summarizes the results of all attacking modules and gives the recommendations for the secure deployment to avoid possible threats.Publication Metadata only Surface plasmon resonance imaging for ABH antigen detection on red blood cells and in saliva: Secretor status-related ABO subgroup identification(2017-05-07) Patjaree Peungthum; Krisda Sudprasert; Ratthasart Amarit; Armote Somboonkaew; Boonsong Sutapun; Apirom Vongsakulyanon; Wuttigrai Seedacoon; Pimpun Kitpoka; Mongkol Kunakorn; Toemsak Srikhirin; Mahidol University; Thailand National Electronics and Computer Technology Center; Suranaree University of Technology© 2017 The Royal Society of Chemistry. Low antigenic expression of ABO subgroup system on red blood cell (RBC) is cause of discrepancy between forward and reverse blood typing in the standard agglutination technique. Neutralization agglutination is employed for verification of the detection of ABH substances in saliva. However, the neutralization technique is complicated, time-consuming and requires expertise. To overcome these drawbacks, surface plasmon resonance (SPR) imaging was developed for ABH antigen detection on RBCs and in saliva. An antibody array was designed to classify the ABO subgroups by anti-A, anti-B, and anti-H antibodies; the array was immobilized on a carboxymethyl-dextran sensor-surface. RBCs and saliva specimens from sixty-four donors were analysed by passing them over the antibody array, where the secretor status and blood group could be simultaneously identified. Consequently, the immobilized antibodies could specifically and quantitatively detect the ABH antigen on RBCs. Using the direct assay, the SPR signal of saliva detection was weaker than that of RBC detection. However, a sandwich assay with a mixture of anti-A, anti-B, and anti-H antibodies could efficiently enhance the signal. The sensor chip provided high specificity (cut-off at 100 to 175 micro refractive index units) and high precision at 0.06%-4.9% CV. The blood group results of the sixty-four donor specimens obtained by SPR agreed with the standard agglutination test with 100% accuracy. SPR could indicate different ABH antigen densities on the RBCs and nearly the same amounts of ABH substances in the saliva of strong and weak subgroups. Finally, we also demonstrated reduced assay time and fewer complications with the SPR imaging platform compared to the neutralization technique.Publication Metadata only Influence of Vegetation Cover on Nest Cavity Selection and Nesting Success of White-Rumped Shamas (Copsychus malabaricus): An Experimental Test(2017-12-01) Tananun Chotprasertkoon; Andrew J. Pierce; Tommaso Savini; Philip D. Round; Wangworn Sankamethawee; George A. Gale; King Mongkut s University of Technology Thonburi; Mahidol University; Khon Kaen University© 2017 Wilson Ornithological Society. All rights reserved. It is often assumed that passerine birds conceal nests to reduce predation because nest predation is usually the primary cause of reproductive failure. We hypothesized that nesting success would be higher in nest cavities with more concealed entrances and therefore more likely chosen over less concealed cavities. We set 200 nest boxes in ∼100 territories of White-rumped Shamas (Copsychus malabaricus), territorial cavity nesters, in a seasonal evergreen forest in northeastern Thailand. Shamas were provided two boxes per territory, one with high and the other with low vegetation cover in front of the box entrance. Boxes were reset in new locations after a nest fledged or failed, providing 170 'choice' trials. We also compared vegetation characteristics of natural nest cavities with available natural nest cavities. Contrary to predictions, shamas placed nest material in the less concealed boxes significantly more often (89 boxes vs. 48), were significantly more likely to lay eggs in less concealed boxes (66 vs. 27), and eggs were significantly more likely to hatch in the low concealment treatment (38 vs. 8). Nest survival in the low concealment treatment was approximately twice that of the highly concealed boxes 36% (± 0.035) vs. 18% (± 0.042). Vegetation around natural nests (n = 26) was not significantly different from available randomly selected cavities (n = 52). Both used and available cavities had low levels of concealment. Cavity-nesting species may prefer less concealed sites because it is easier for an incubating/brooding female to detect approaching predators, allowing additional time for escaping and/or for mounting a defense.Publication Metadata only iCare-stress: Caring system for stress(2017-10-19) Jarernsri Mitrpanont; Jaruwan Phandhu-Fung; Nantanut Klubdee; Supanat Ratanalaor; Prasert Pratiphakorn; Kiddathikarn Damrongvanakul; Piyawat Chuanvaree; Teeranan Mitrpanont; Mahidol University© 2017 IEEE. The human life becomes increasingly stressful and not everyone can manage his/her own life well. Most people are not aware of stress even though stress is a common illness that impacts on daily life, including family, relationships, and studying. Moreover, stress affects health, both physically and mentally at all ages. When people suffer from stress repeatedly, stress will turn to be multiple physical conditions and psychological problems such as anxiety and depression. iCare-stress application was created to assist a person to know his/her own state of stress and handle properly using the user's brainwave detected by Emotiv device. In addition, the application provides an interesting technique called Neurofeedback which is used to train human brain activity. Neurofeedback is widely accepted in medicinal studies to reduce the symptoms of many diseases. Therefore, it is chosen to be the technique to manage and reduce stress in the application. Moreover, there are three interventions for doing the different activities such as Concentration, Relaxation, and Music Therapy. In the case of measuring stress without the device, the application provides a set of questionnaire which is called General Health Questionnaire or GHQ that a user can apply to check his/her stress level. The result from both doing interventions and GHQ are shown in the form of human language that anyone can understand.