Publication: Oleic acid Enhances Dengue Virus But Not Dengue Virus-Like Particle Production from Mammalian Cells
Issued Date
2017-10-01
Resource Type
ISSN
15590305
10736085
10736085
Other identifier(s)
2-s2.0-85027057072
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Molecular Biotechnology. Vol.59, No.9-10 (2017), 385-393
Suggested Citation
Suwipa Ramphan, Sathiporn Suksathan, Nitwara Wikan, Puey Ounjai, Kanpong Boonthaworn, Poramate Rimthong, Tapanee Kanjanapruthipong, Suchin Worawichawong, Anan Jongkaewwattana, Nuttaporn Wongsiriroj, Duncan R. Smith Oleic acid Enhances Dengue Virus But Not Dengue Virus-Like Particle Production from Mammalian Cells. Molecular Biotechnology. Vol.59, No.9-10 (2017), 385-393. doi:10.1007/s12033-017-0029-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41776
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Oleic acid Enhances Dengue Virus But Not Dengue Virus-Like Particle Production from Mammalian Cells
Other Contributor(s)
Abstract
© 2017, Springer Science+Business Media, LLC. Despite the recent introduction of a commercial vaccine, the mosquito-transmitted dengue virus is still a worldwide public health problem. Based on the live attenuated vaccine strategy, the commercial vaccine has a less than optimal protective profile. Virus-like particles (VLPs) offer an attractive alternate vaccination strategy due to the effectively native presentation of epitopes in the absence of any infectious genetic material. However, the production of amounts of VLP in a platform that can support commercial development remains a major obstacle. This study generated two DENV 2 VLPs [codon-optimized and chimeric DENV/Japanese encephalitis virus (JEV)] and directly compared yields of these constructs by western blotting and dot blot hybridization. The effect of oleic acid supplementation, a process known to increase DENV production in natural infection, was also investigated. Results showed that the chimeric construct gave a two- to threefold higher yield than the codon-optimized construct and that while oleic acid increased DENV virion production in natural infection, it inhibited VLP production. These results suggest that further optimization of DENV VLP expression is possible, but it will require more understanding of how native DENV infection remodels the host cell machinery.