Publication:
Characterization of HIV-1 gp120 antibody specificities induced in anogenital secretions of RV144 vaccine recipients after late boost immunizations

Issued Date

2018-04-01

Resource Type

Article

ISSN

19326203

DOI

10.1371/journal.pone.0196397

Other identifier(s)

2-s2.0-85046090819

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Mahidol University

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SCOPUS

Bibliographic Citation

PLoS ONE. Vol.13, No.4 (2018)

Suggested Citation

Siriwat Akapirat, Chitraporn Karnasuta, Sandhya Vasan, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sirinan Madnote, Hathairat Savadsuk, Surawach Rittiroongrad, Jiraporn Puangkaew, Sanjay Phogat, James Tartaglia, Faruk Sinangil, Mark S. de Souza, Jean Louis Excler, Jerome H. Kim, Merlin L. Robb, Nelson L. Michael, Viseth Ngauy, Robert J. O’Connell, Nicos Karasavvas Characterization of HIV-1 gp120 antibody specificities induced in anogenital secretions of RV144 vaccine recipients after late boost immunizations. PLoS ONE. Vol.13, No.4 (2018). doi:10.1371/journal.pone.0196397 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/44788

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Title

Characterization of HIV-1 gp120 antibody specificities induced in anogenital secretions of RV144 vaccine recipients after late boost immunizations

Author(s)

Siriwat Akapirat
 Chitraporn Karnasuta
 Sandhya Vasan
 Supachai Rerks-Ngarm
 Punnee Pitisuttithum
 Sirinan Madnote
 Hathairat Savadsuk
 Surawach Rittiroongrad
 Jiraporn Puangkaew
 Sanjay Phogat
 James Tartaglia
 Faruk Sinangil
 Mark S. de Souza
 Jean Louis Excler
 Jerome H. Kim
 Merlin L. Robb
 Nelson L. Michael
 Viseth Ngauy
 Robert J. O’Connell
 Nicos Karasavvas

Other Contributor(s)

International Vaccine Institute, Seoul
 National Institute of Infectious Diseases
 Armed Forces Research Institute of Medical Sciences, Thailand
 Thailand Ministry of Public Health
 HJF
 Walter Reed Army Institute of Research
 Mahidol University
 Thai Red Cross AIDS Research Centre
 Global Solutions for Infectious Diseases
 Sanofi Pasteur

Abstract

© This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Sexual transmission is the principal driver of the human immunodeficiency virus (HIV) pandemic. Understanding HIV vaccine-induced immune responses at mucosal surfaces can generate hypotheses regarding mechanisms of protection, and may influence vaccine development. The RV144 (ClinicalTrials.gov NCT00223080) efficacy trial showed protection against HIV infections but mucosal samples were not collected, therefore, the contribution of mucosal antibodies to preventing HIV-1 acquisition is unknown. Here, we report the generation, magnitude and persistence of antibody responses to recombinant gp120 envelope and antigens including variable one and two loop scaffold antigens (gp70V1V2) previously shown to correlate with risk in RV144. We evaluated antibody responses to gp120 A244gD and gp70V1V2 92TH023 (both CRF01_AE) and Case A2 (subtype B) in cervico-vaginal mucus (CVM), seminal plasma (SP) and rectal secretions (RS) from HIV-uninfected RV144 vaccine recipients, who were randomized to receive two late boosts of ALVAC-HIV/ AIDSVAX®B/E, AIDSVAX®B/E, or ALVAC-HIV alone at 0 and 6 months. Late vaccine boosting increased IgG geometric mean titers (GMT) to gp120 A244gD in AIDSVAX®B/E and ALVAC-HIV/AIDSVAX®B/E CVM (28 and 17 fold, respectively), followed by SP and RS. IgG to gp70V1V2 92TH023 increased in AIDSVAX®B/E and ALVAC-HIV/AIDSVAX®B/E CVM (11–17 fold) and SP (2 fold) two weeks post first boost. IgG to Case A2 was only detected in AIDSVAX®B/E and ALVAC-HIV/AIDSVAX®B/E CVM. Mucosal IgG to gp120 A244gD (CVM, SP, RS), gp70V1V2 92TH023 (CVM, SP), and Case A2 (CVM) correlated with plasma IgG levels (p<0.001). Although the magnitude of IgG responses declined after boosting, anti-gp120 A244gD IgG responses in CVM persisted for 12 months post final vaccination. Further studies in localization, persistence and magnitude of envelope specific antibodies (IgG and dimeric IgA) in anogenital secretions will help determine their role in preventing mucosal HIV acquisition.

Keyword(s)

Agricultural and Biological Sciences
 Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/44788

Collections

Scopus 2018