Publication: Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death
Issued Date
2013-09-17
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ISSN
17560500
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2-s2.0-84883658015
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Mahidol University
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SCOPUS
Bibliographic Citation
BMC Research Notes. Vol.6, No.1 (2013)
Suggested Citation
Chutima Thepparit, Atefeh Khakpoor, Sarawut Khongwichit, Nitwara Wikan, Chanida Fongsaran, Pimjai Chingsuwanrote, Patcharee Panraksa, Duncan R. Smith Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death. BMC Research Notes. Vol.6, No.1 (2013). doi:10.1186/1756-0500-6-372 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31214
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Title
Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death
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Abstract
Background: A number of studies have implicated the direct involvement of the liver in dengue virus (DENV) infection, and it has been widely shown that liver cells subsequently undergo apoptosis. The mechanism by which liver cells undergo apoptosis in response to DENV infection remains unclear. To provide further information on the mechanism of apoptosis in DENV infected liver cells, HepG2 cells were infected with DENV 2 and analyzed for the induction of ER stress, apoptosis and autophagy. Results: In response to DENV infection, HepG2 cells showed the induction of both the ER resident unfolded protein response as well as the Noxa/PUMA stress response pathways. Proteolytic activation of caspases 4, 7, 8 and 9 was observed as well as changes in mitochondrial transmembrane potential. Increased monodansylcadaverine staining was observed in DENV infected cells, consistent with the previously reported induction of autophagy. Conclusions: These results are consistent with a model in which the induction of multiple ER stress pathways is coupled with the induction of multiple cell death pathways as a mechanism to ensure the removal of infected liver cells from the system. © 2013 Thepparit et al.; licensee BioMed Central Ltd.