Publication: Opisthorchis viverrini: Ultrastructure and cytochemistry of the glycocalyx of the tegument
Issued Date
2000-03-01
Resource Type
ISSN
0022149X
Other identifier(s)
2-s2.0-0034127926
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Helminthology. Vol.74, No.1 (2000), 23-29
Suggested Citation
W. Apinhasmit, P. Sobhon, C. Tarasub, W. Mothong, P. Saitongdee, P. Sretarugsa, C. Wanichanon, E. S. Upatham Opisthorchis viverrini: Ultrastructure and cytochemistry of the glycocalyx of the tegument. Journal of Helminthology. Vol.74, No.1 (2000), 23-29. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/25806
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Opisthorchis viverrini: Ultrastructure and cytochemistry of the glycocalyx of the tegument
Other Contributor(s)
Abstract
The ultrastructure and cytochemistry of the glycocalyx of the tegument of Opisthorchis viverrini during maturation from newly excysted juvenile to adult stages were investigated using colloidal iron, ruthenium red and lectin stainings. The results showed that the glycocalyx was intensely stained by the first two dyes, thus indicating the presence of relatively high amounts of negative charges. However, the thickness and intensity of the staining decreased during the fluke's maturation. Binding studies using lectin probes on the surface of adult parasites showed that binding sites for Canavalia ensiformis (Con A), Triticum vulgaris (WGA) and Ricinus communis I (RCA I) were present in relative large amounts on the glycocalyx of the adult tegument, whereas those for Dolichos biflorus (DBA) were relatively fewer in number, and those for Ulex europaeus I (UEA I) were absent. The binding patterns of Con A, WGA, RCA I and DBA were generally similar, and the reaction product was uniformly distributed over the dorsal and ventral surfaces of the parasite's body. These bindings, therefore, indicate the presence of D-mannose/D-glucose, N-acetyl-D-glucosamine/sialic acid, D-galactose and N-acetyl-D-galactosamine residues on the glycocalyx of the adult tegument.