Publication: Antitumor efficacy and intratumoral distribution of SN-38 from polymeric depots in brain tumor model
1
Issued Date
2015-12-01
Resource Type
ISSN
15353699
15353702
15353702
Other identifier(s)
2-s2.0-84958259115
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Experimental Biology and Medicine. Vol.240, No.12 (2015), 1640-1647
Suggested Citation
Ketpat Vejjasilpa, Norased Nasongkla, Chawan Manaspon, Noppadol Larbcharoensub, Atthaporn Boongird, Suradej Hongeng, Nipan Israsena Antitumor efficacy and intratumoral distribution of SN-38 from polymeric depots in brain tumor model. Experimental Biology and Medicine. Vol.240, No.12 (2015), 1640-1647. doi:10.1177/1535370215590819 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35339
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antitumor efficacy and intratumoral distribution of SN-38 from polymeric depots in brain tumor model
Other Contributor(s)
Abstract
© 2015, © 2015 by the Society for Experimental Biology and Medicine. We investigate antitumor efficacy and 2D and 3D intratumoral distribution of 7-ethyl-10-hydroxycamptothecin (SN-38) from polymeric depots inside U-87MG xenograft tumor model in nude mice. Results showed that polymeric depots could be used to administer and controlled release of a large amount of SN-38 directly to the brain tumor model. SN-38 released from depots suppressed tumor growth, where the extent of suppression greatly depended on doses and the number of depot injections. Tumor suppression of SN-38 from depots was three-fold higher in animals which received double injections of depots at high dose (9.7 mg of SN-38) compared to single injection (2.2 mg). H&E staining of tumor sections showed that the area of tumor cell death/survival of the former group was two-fold higher than those of the latter group. Fluorescence imaging based on self-fluorescent property of SN-38 was used to evaluate the intratumoral distribution of this drug compared to histological results. The linear correlation between fluorescence intensity and the amount of SN-38 allowed quantitative determination of SN-38 in tumor tissues. Results clearly showed direct correlation between the amount of SN-38 in tumor sections and cancer cell death. Moreover, 3D reconstruction representing the distribution of SN-38 in tumors was obtained. Results from this study suggest the rationale for intratumoral drug administration and release of drugs inside tumor, which is necessary to design drug delivery systems with efficient antitumor activity.