Publication: Cytochrome oxidase 2 (Cox2), β-tubulin (tub) and chitin synthase subunit 2 (chs2) expression in pythium insidiosum thai strains
Issued Date
2021-01-01
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ISSN
2228835X
16863933
16863933
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2-s2.0-85105214306
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Mahidol University
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SCOPUS
Bibliographic Citation
Walailak Journal of Science and Technology. Vol.18, No.8 (2021)
Suggested Citation
Patcharee Kammarnjassadakul, Watcharin Rangsipanuratn, Methee Sriprapun, Popchai Ngamsakulrungruj, Tanapat Palaga, Kallaya Sritunyalucksana, Ariya Chindamporn Cytochrome oxidase 2 (Cox2), β-tubulin (tub) and chitin synthase subunit 2 (chs2) expression in pythium insidiosum thai strains. Walailak Journal of Science and Technology. Vol.18, No.8 (2021). doi:10.48048/wjst.2021.9433 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79399
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Title
Cytochrome oxidase 2 (Cox2), β-tubulin (tub) and chitin synthase subunit 2 (chs2) expression in pythium insidiosum thai strains
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Abstract
This study aimed to investigate the real-time fluctuation of temperature-sensitive gene expression in a Pythium insidiosum growing at human body temperature (37 ºC) when it can cause life-threatening disease, whereas its natural habitat is in agricultural water sources with lower temperature. Genes encoding cytochrome oxidase 2 (COX2), β-tubulin (TUB), and chitin synthase subunit 2 (CHS2) were tested for their expression at the infection temperature (37 ºC) and natural habitat temperature (27 ºC) using real-time RT-PCR. Sixteen strains of P. insidiosum, representing 3 phylogeographic preferences, were analyzed. The subculturing process at 27 ºC was repeated at least 2-3 cycles. The results showed that at 37 ºC, the COX2 was expressed 2.5-fold higher than that of 27 ºC (p = 0.0347) while the level of TUB and CHS2 mRNA disclosed indistinguishable profiles. These results suggested that these genes were suitable to be used as housekeeping genes for temperature-susceptible gene expression studies. This work was likely to be the 1st study examining the gene expression levels of the Thai strain of P. insidiosum under thermal stress conditions. Follow-up studies for the COX2 genes may useful to provide valuable insight into the pathogenesis, diagnostic, or therapeutic targets for further investigation.