Publication: A comparative study of the cemental surfaces of teeth with and without periodontal diseases by scanning electron microscopy.
Accepted Date
2014-07-15
Issued Date
2014-09
Resource Type
Language
eng
ISSN
0125-5614 (printed)
Rights
Mahidol University
Rights Holder(s)
Faculty of Dentistry Mahidol University
Bibliographic Citation
Worawongvasu R. A comparative study of the cemental surfaces of teeth with and without periodontal diseases by scanning electron microscopy. M Dent J. 2014; 34(3): 253-62.
Suggested Citation
Ratthapong Worawongvasu, รัฐพงษ์ วรวงศ์วสุ A comparative study of the cemental surfaces of teeth with and without periodontal diseases by scanning electron microscopy.. Worawongvasu R. A comparative study of the cemental surfaces of teeth with and without periodontal diseases by scanning electron microscopy. M Dent J. 2014; 34(3): 253-62.. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/1128
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Title
A comparative study of the cemental surfaces of teeth with and without periodontal diseases by scanning electron microscopy.
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Abstract
Objectives: The purpose of this study was to compare the cemental surfaces
of normal teeth with those of teeth with periodontitis by scanning electron
microscopy.
Materials and methods: Five normal premolars and 5 premolars with
periodontitis were used in this study. The specimens were collected from the
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol
University and private dental clinics. After extractions, the teeth were stored
in 10% formalin until required. The specimens were cut in mesiodistal
directions, immersed in 5.25% sodium hypochlorite and then dehydrated
using increasing ethanol concentrations (two changes 15 minutes each): 50%,
60%, 70%, 85%, 95%, 100% and dried by leaving the specimens at room
temperature for 24 hours, mounted on aluminum stubs, coated with gold,
100-300 Å thick, with an ion sputter coater, and viewed with a JEOL JSM-6610
LV scanning electron microscope, at three magnifications: X500, X1,000, and X
2,000. The photomicrographs were described and compared between 2
groups.
Results: The nondiseased roots showed an area with a pebbly appearance.
Sharpey fibers appeared as low confluent mounds and dish-topped
projections. The surfaces of periodontally diseased roots were uneven pebbly.
The cemental surfaces appeared as numerous low confluent mounds or
dish-topped projections and were covered by irregular, thin and sheet-like
deposits. Discrete clusters of spherical mineral particles were distributed over
the surfaces of the mounds. Each mound was low and consisted of
close-packed Sharpey fibers. In between mounds, presence of some intrinsic
matrix fibers parallel with the surface was clearly seen. In some areas, some
elevations were dish-topped and contained intrinsic matrix fibers parallel with
the surface. In areas of dense deposits, the mineral particles appeared as
spherical and irregular thin sheet-like deposits. An extensive area of the root
covered by irregular thin sheet-like deposits was seen. In many roots, large,
multiloculated resorption bays were seen.
Conclusions: The root surfaces of the normal teeth show no calculus
retention and no destruction of the cemental surfaces, but those of the teeth
with chronic periodontitis show cemental surfaces which are full of calculus
retention and resorption areas which are large and widely distributed.