Publication: Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: Homology-based 3D model and implications for toxin activity
4
Issued Date
2004-05-31
Resource Type
ISSN
12258687
Other identifier(s)
2-s2.0-2642587327
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Biochemistry and Molecular Biology. Vol.37, No.3 (2004), 304-313
Suggested Citation
Chanan Angsuthanasombat, Panapat Uawithya, Somphob Leetachewa, Walairat Pornwiroon, Puey Ounjai, Teerakiat Kerdcharoen, Gerd Katzenmeier, Sakol Panyim Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: Homology-based 3D model and implications for toxin activity. Journal of Biochemistry and Molecular Biology. Vol.37, No.3 (2004), 304-313. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21182
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: Homology-based 3D model and implications for toxin activity
Other Contributor(s)
Abstract
Three-dimensional (3D) models for the 65-kDa activated Cry4A and Cry4B δ-endotoxins from Bacillus thuringienis subsp. israelensis that are specifically toxic to mosquito-larvae were constructed by homology modeling, based on atomic coordinates of the CrylAa and Cry3Aa crystal structures. They were structurally similar to the known structures, both derived 3D models displayed a three-domain organization: the N-terminal domain (I) is a seven-helix bundle, while the middle and C-terminal domains are primarily comprise of anti-parallel β-sheets. Circular dichroism spectroscopy confirmed the secondary structural contents of the two homology-based Cry4 structures. A structural analysis of both Cry4 models revealed the following: (a) Residues Arg-235 and Arg-203 are located in the interhelical 5/6 loop within the domain I of Cry4A and Cry4B, respectively. Both are solvent exposed. This suggests that they are susceptible to tryptic cleavage. (b) The unique disulphide bond, together with a proline-rich region within the long loop connecting α4 and α5 of Cry4A, were identified. This implies their functional significance for membrane insertion. (c) Significant structural differences between both models were found within domain II that may reflect their different activity spectra. Structural insights from this molecular modeling study would therefore increase our understanding of the mechanic aspects of these two closely related mosquito-larvicidal proteins.